Matches in SemOpenAlex for { <https://semopenalex.org/work/W2087197375> ?p ?o ?g. }
Showing items 1 to 88 of
88
with 100 items per page.
- W2087197375 abstract "Septins have been discovered more than 30 years ago as temperature sensitive mutants in budding yeast Saccharomyces Cerevisiae. Septins make an hourglass shaped structure of filaments bound to the inner cell membrane. Mitotic budding yeasts express five septins: Cdc3, Cdc10, Cdc11, Cdc12, and Shs1/Sep7. All, but Shs1, are essential for cell division.Using electron microscopy of negatively-stained samples, in vitro, we have observed that the Cdc3-Cdc10-Cdc11-Cdc12 septin complex self-assemble into octameric rods in high salt. At lower ionic strength, septins polymerize into paired filaments. The position and identity of each subunit in the rod has been determined. This analysis revealed a symmetric organization where the different subunits are arranged in the following order: Cdc11-Cdc12-Cdc3-Cdc10-Cdc10-Cdc3-Cdc12-Cdc11. We have also shown that the subunit-subunit interfaces alternate between so called N-C and G interfaces.To get more insight into septin organization in situ we have studied septin-lipid interaction using a lipid monolayer model assay. We have seen that budding yeast septins interact specifically with (PI(4,5)P2). This interaction promotes filament formation and organization, even for mutants or under conditions where septins do not polymerize in solution. This interaction appears to be specifically mediated through Cdc10 and Cdc3.We have been also analyzed the organization of septin filaments in situ, using electron tomography to visualized dividing budding yeast cells. 3D reconstructions of yeast sections were obtained by electron tomography using either freeze substituted samples or cryo-sections (Cemovis). Surprisingly, an array of two sets of perpendicular filaments is present at the bud neck. Cells displaying different types of septin mutations are now being analyzed." @default.
- W2087197375 created "2016-06-24" @default.
- W2087197375 creator A5013371396 @default.
- W2087197375 creator A5034946559 @default.
- W2087197375 creator A5041019545 @default.
- W2087197375 creator A5047107442 @default.
- W2087197375 creator A5048920959 @default.
- W2087197375 creator A5059374123 @default.
- W2087197375 date "2010-01-01" @default.
- W2087197375 modified "2023-09-30" @default.
- W2087197375 title "Ultrastructural Organization of Budding Yeast Septin Filaments Both in vitro and in situ, Analyzed by Electron Microscopy" @default.
- W2087197375 doi "https://doi.org/10.1016/j.bpj.2009.12.2069" @default.
- W2087197375 hasPublicationYear "2010" @default.
- W2087197375 type Work @default.
- W2087197375 sameAs 2087197375 @default.
- W2087197375 citedByCount "1" @default.
- W2087197375 countsByYear W20871973752020 @default.
- W2087197375 crossrefType "journal-article" @default.
- W2087197375 hasAuthorship W2087197375A5013371396 @default.
- W2087197375 hasAuthorship W2087197375A5034946559 @default.
- W2087197375 hasAuthorship W2087197375A5041019545 @default.
- W2087197375 hasAuthorship W2087197375A5047107442 @default.
- W2087197375 hasAuthorship W2087197375A5048920959 @default.
- W2087197375 hasAuthorship W2087197375A5059374123 @default.
- W2087197375 hasBestOaLocation W20871973751 @default.
- W2087197375 hasConcept C104292427 @default.
- W2087197375 hasConcept C104317684 @default.
- W2087197375 hasConcept C120665830 @default.
- W2087197375 hasConcept C121332964 @default.
- W2087197375 hasConcept C12554922 @default.
- W2087197375 hasConcept C142669718 @default.
- W2087197375 hasConcept C1491633281 @default.
- W2087197375 hasConcept C163716698 @default.
- W2087197375 hasConcept C179411191 @default.
- W2087197375 hasConcept C198410946 @default.
- W2087197375 hasConcept C2777576037 @default.
- W2087197375 hasConcept C2777993257 @default.
- W2087197375 hasConcept C2779222958 @default.
- W2087197375 hasConcept C45472230 @default.
- W2087197375 hasConcept C55493867 @default.
- W2087197375 hasConcept C85813293 @default.
- W2087197375 hasConcept C86803240 @default.
- W2087197375 hasConcept C95444343 @default.
- W2087197375 hasConceptScore W2087197375C104292427 @default.
- W2087197375 hasConceptScore W2087197375C104317684 @default.
- W2087197375 hasConceptScore W2087197375C120665830 @default.
- W2087197375 hasConceptScore W2087197375C121332964 @default.
- W2087197375 hasConceptScore W2087197375C12554922 @default.
- W2087197375 hasConceptScore W2087197375C142669718 @default.
- W2087197375 hasConceptScore W2087197375C1491633281 @default.
- W2087197375 hasConceptScore W2087197375C163716698 @default.
- W2087197375 hasConceptScore W2087197375C179411191 @default.
- W2087197375 hasConceptScore W2087197375C198410946 @default.
- W2087197375 hasConceptScore W2087197375C2777576037 @default.
- W2087197375 hasConceptScore W2087197375C2777993257 @default.
- W2087197375 hasConceptScore W2087197375C2779222958 @default.
- W2087197375 hasConceptScore W2087197375C45472230 @default.
- W2087197375 hasConceptScore W2087197375C55493867 @default.
- W2087197375 hasConceptScore W2087197375C85813293 @default.
- W2087197375 hasConceptScore W2087197375C86803240 @default.
- W2087197375 hasConceptScore W2087197375C95444343 @default.
- W2087197375 hasLocation W20871973751 @default.
- W2087197375 hasOpenAccess W2087197375 @default.
- W2087197375 hasPrimaryLocation W20871973751 @default.
- W2087197375 hasRelatedWork W1970717957 @default.
- W2087197375 hasRelatedWork W1974073543 @default.
- W2087197375 hasRelatedWork W2030819210 @default.
- W2087197375 hasRelatedWork W2037747220 @default.
- W2087197375 hasRelatedWork W2040896118 @default.
- W2087197375 hasRelatedWork W2054317237 @default.
- W2087197375 hasRelatedWork W2080726128 @default.
- W2087197375 hasRelatedWork W2082628132 @default.
- W2087197375 hasRelatedWork W2100558257 @default.
- W2087197375 hasRelatedWork W2117239697 @default.
- W2087197375 hasRelatedWork W2127422455 @default.
- W2087197375 hasRelatedWork W2134438684 @default.
- W2087197375 hasRelatedWork W2164863856 @default.
- W2087197375 hasRelatedWork W2166255678 @default.
- W2087197375 hasRelatedWork W2218185345 @default.
- W2087197375 hasRelatedWork W2417721535 @default.
- W2087197375 hasRelatedWork W2619417242 @default.
- W2087197375 hasRelatedWork W2625635356 @default.
- W2087197375 hasRelatedWork W3022210519 @default.
- W2087197375 hasRelatedWork W3088899258 @default.
- W2087197375 isParatext "false" @default.
- W2087197375 isRetracted "false" @default.
- W2087197375 magId "2087197375" @default.
- W2087197375 workType "article" @default.