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- W2087212002 abstract "The formation and biological function of small ribosomal subparticles in Escherichia coli Bs-1 irradiated with ultraviolet light were investigated. The experimental results are as follows: 1. Ultraviolet irradiation inhibited both the formation of 50-S ribosomal subunits in E. coli Bs-1 and the synthesis of 23-S rRNA. 2. No recovery of the synthesis of either 50-S ribosomal subunits or 23-S rRNA was found in ultraviolet-irradiated E. coli Bs-1 even when the bacteria were incubated for 2 h after the irradiation. 3. Synthesis of 16-S rRNA was resistant to ultraviolet irradiation, and synthesis of ribonucleoprotein particles did not correspond to the smaller counterpart in 70-S ribosomes. However, the ribonucleoprotein particles formed were always smaller than 30 S. Higher ultraviolet doses formed smaller particles; the smallest particles observed were about 20 S. 4. The sedimentation coefficient of the RNA component of these small particles was 16 S in all cases. The base composition of the 16-S RNA of small particles did not differ from that of normal 16-S rRNA which was the RNA component of 30-S ribosomal subunits. The base sequences of both 16-S RNA and 16-S rRNA were identical. 5. The small particles were not able to form complexes with 50-S ribosomal subunits and had no ability to bind poly U and T2 phage-specific mRNA. 6. The amount of protein component in the ribosomal particles was less in the small particles than in normal 30-S ribosomal subunits." @default.
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- W2087212002 title "On the ribosomal subparticles formed in Escherichia coli Bs-1 irradiated with ultraviolet light: Formation and biological function of small particles" @default.
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- W2087212002 doi "https://doi.org/10.1016/0005-2787(69)90491-2" @default.
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