FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2087220625> ?p ?o ?g. }

• W2087220625 endingPage "1016" @default.
• W2087220625 startingPage "1009" @default.
• W2087220625 abstract "Synthetic biology approaches often combine natural building blocks to generate new cellular activities. Here, we make use of two RNA elements to design a regulatory device with novel functionality. The system is based on a hammerhead ribozyme (HHR) that cleaves itself to generate a liberated ribosome-binding site and, thus, permits expression of a downstream gene. We connected a temperature-responsive RNA hairpin to the HHR and, thus, generated a temperature-controlled ribozyme that we call thermozyme. Specifically, a Salmonella RNA thermometer (RNAT) known to modulate small heat shock gene expression by temperature-controlled base-pairing and melting was fused to the ribozyme. Following an in vivo screening approach, we isolated two functional thermozymes. In vivo expression studies and in vitro structure probing experiments support a mechanism in which rising temperatures melt the thermometer structure impairing the self-cleavage reaction of the ribozyme. Since RNA cleavage is necessary to liberate the RBS, these engineered thermozymes shut off gene expression in response to a temperature increase and, thus, act in a reverse manner as the natural RNAT. Our results clearly emphasize the highly modular nature and biotechnological potential of ribozyme-based RNA thermometers." @default.
• W2087220625 created "2016-06-24" @default.
• W2087220625 creator A5003584263 @default.
• W2087220625 creator A5016867920 @default.
• W2087220625 creator A5036822997 @default.
• W2087220625 creator A5077253315 @default.
• W2087220625 creator A5079185955 @default.
• W2087220625 date "2013-06-01" @default.
• W2087220625 modified "2023-09-26" @default.
• W2087220625 title "Thermozymes" @default.
• W2087220625 cites W1549643145 @default.
• W2087220625 cites W1967635084 @default.
• W2087220625 cites W1972139720 @default.
• W2087220625 cites W1976851914 @default.
• W2087220625 cites W1985492085 @default.
• W2087220625 cites W1987601967 @default.
• W2087220625 cites W1988517965 @default.
• W2087220625 cites W1989297603 @default.
• W2087220625 cites W1993191354 @default.
• W2087220625 cites W2007909703 @default.
• W2087220625 cites W2014284717 @default.
• W2087220625 cites W2016206030 @default.
• W2087220625 cites W2023614447 @default.
• W2087220625 cites W2030621944 @default.
• W2087220625 cites W2034328637 @default.
• W2087220625 cites W2047946060 @default.
• W2087220625 cites W2049754925 @default.
• W2087220625 cites W2053272506 @default.
• W2087220625 cites W2058905055 @default.
• W2087220625 cites W2069961460 @default.
• W2087220625 cites W2075452050 @default.
• W2087220625 cites W2076843089 @default.
• W2087220625 cites W2076937215 @default.
• W2087220625 cites W2092541496 @default.
• W2087220625 cites W2093417734 @default.
• W2087220625 cites W2106997302 @default.
• W2087220625 cites W2113582742 @default.
• W2087220625 cites W2122282339 @default.
• W2087220625 cites W2123902202 @default.
• W2087220625 cites W2131841865 @default.
• W2087220625 cites W2134499928 @default.
• W2087220625 cites W2140098818 @default.
• W2087220625 cites W2142157992 @default.
• W2087220625 cites W2144759995 @default.
• W2087220625 cites W2146669791 @default.
• W2087220625 cites W2157052506 @default.
• W2087220625 cites W2158842205 @default.
• W2087220625 cites W2162920755 @default.
• W2087220625 cites W2167390230 @default.
• W2087220625 cites W2167486503 @default.
• W2087220625 cites W2167493273 @default.
• W2087220625 cites W2260215781 @default.
• W2087220625 doi "https://doi.org/10.4161/rna.24482" @default.
• W2087220625 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4111729" @default.
• W2087220625 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/23595083" @default.
• W2087220625 hasPublicationYear "2013" @default.
• W2087220625 type Work @default.
• W2087220625 sameAs 2087220625 @default.
• W2087220625 citedByCount "36" @default.
• W2087220625 countsByYear W20872206252014 @default.
• W2087220625 countsByYear W20872206252015 @default.
• W2087220625 countsByYear W20872206252016 @default.
• W2087220625 countsByYear W20872206252017 @default.
• W2087220625 countsByYear W20872206252018 @default.
• W2087220625 countsByYear W20872206252019 @default.
• W2087220625 countsByYear W20872206252021 @default.
• W2087220625 countsByYear W20872206252022 @default.
• W2087220625 crossrefType "journal-article" @default.
• W2087220625 hasAuthorship W2087220625A5003584263 @default.
• W2087220625 hasAuthorship W2087220625A5016867920 @default.
• W2087220625 hasAuthorship W2087220625A5036822997 @default.
• W2087220625 hasAuthorship W2087220625A5077253315 @default.
• W2087220625 hasAuthorship W2087220625A5079185955 @default.
• W2087220625 hasBestOaLocation W20872206251 @default.
• W2087220625 hasConcept C104317684 @default.
• W2087220625 hasConcept C107745601 @default.
• W2087220625 hasConcept C12554922 @default.
• W2087220625 hasConcept C148625055 @default.
• W2087220625 hasConcept C150194340 @default.
• W2087220625 hasConcept C191908910 @default.
• W2087220625 hasConcept C194993378 @default.
• W2087220625 hasConcept C2779792011 @default.
• W2087220625 hasConcept C54355233 @default.
• W2087220625 hasConcept C66144319 @default.
• W2087220625 hasConcept C67705224 @default.
• W2087220625 hasConcept C70721500 @default.
• W2087220625 hasConcept C76346623 @default.
• W2087220625 hasConcept C86803240 @default.
• W2087220625 hasConcept C95444343 @default.
• W2087220625 hasConceptScore W2087220625C104317684 @default.
• W2087220625 hasConceptScore W2087220625C107745601 @default.
• W2087220625 hasConceptScore W2087220625C12554922 @default.
• W2087220625 hasConceptScore W2087220625C148625055 @default.
• W2087220625 hasConceptScore W2087220625C150194340 @default.
• W2087220625 hasConceptScore W2087220625C191908910 @default.
• W2087220625 hasConceptScore W2087220625C194993378 @default.
• W2087220625 hasConceptScore W2087220625C2779792011 @default.
• W2087220625 hasConceptScore W2087220625C54355233 @default.

• next