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• W2087339428 abstract "A hybrid tandem instrument of BEqQ geometry was used to determine high-energy decomposition of protonated peptides, such as side-chain fragmentation yielding dn and wn ions. The transmission through both E and Q of such product ions, formed in the second field-free region, permits improved mass resolution and confident mass assignment. The experimental technique may involve synchronous scanning of E and Q, or, for the purpose of identification of specific products, limited-range scanning of either E or Q with the other analyzer fixed. These techniques are not equivalent, with respect to product ion transmission, to the double focusing of product ions achieved with four-sector instruments but nevertheless represent a critical improvement over conventional mass-analyzed ion kinetic energy spectrometry analyses. Fragmentation of protonated peptides occurring in the second field-free region inside and outside the collision cell were distinguished by floating the collision cell above ground potential. Mass filtering using Q confirmed the mass assignments. The data indicate that product ions resulting from spontaneous decomposition are in some instances quantitatively more significant than those resulting from high-energy collisional activation. Furthermore, the differentiation of the products of low- and high-energy processes should facilitate spectral interpretation." @default.
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• W2087339428 date "1993-06-01" @default.
• W2087339428 modified "2023-09-23" @default.
• W2087339428 title "Determination of high-energy fragmentation of protonated peptides using a beqq hybrid mass spectrometer" @default.
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• W2087339428 doi "https://doi.org/10.1016/1044-0305(93)80004-i" @default.
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