Matches in SemOpenAlex for { <https://semopenalex.org/work/W2087370523> ?p ?o ?g. }
- W2087370523 endingPage "35" @default.
- W2087370523 startingPage "27" @default.
- W2087370523 abstract "The scanning electrochemical microscope (SECM) is used to image the activity of enzymes immobilized on the surfaces of disk-shaped carbon-fiber electrodes. SECM was used to map the concentration of enzymatically produced hydroquinone or hydrogen peroxide at the surface of a 33-microm diameter disk-shaped carbon-fiber electrode modified by an immobilized glucose-oxidase layer. Sub-monolayer coverage of the enzyme at the electrode surface could be detected with micrometer resolution. The SECM was also employed as a surface modification tool to produce microscopic regions of enzyme activity by using a variety of methods. One method is a gold-masking process in which microscopic gold patterns act as mask for producing patterns of chemical modification. The gold masks allow operation in both a positive or negative process for patterning enzyme activity. A second method uses the direct mode of the SECM to produce covalently attached amine groups on the carbon surface. The amine groups are anchors for attachment of glucose oxidase by use of a biotin/avidin process. The effect of non-uniform enzyme activity was investigated by using the SECM tip to temporarily damage an immobilized enzyme surface. SECM imaging can observe the spatial extent and time-course of the enzyme recovery process." @default.
- W2087370523 created "2016-06-24" @default.
- W2087370523 creator A5008356841 @default.
- W2087370523 creator A5059811057 @default.
- W2087370523 creator A5074187446 @default.
- W2087370523 date "2001-01-01" @default.
- W2087370523 modified "2023-10-12" @default.
- W2087370523 title "Fabricating and Imaging Carbon-Fiber Immobilized Enzyme Ultramicroelectrodes with Scanning Electrochemical Microscopy" @default.
- W2087370523 cites W1527288608 @default.
- W2087370523 cites W1546907448 @default.
- W2087370523 cites W1749055511 @default.
- W2087370523 cites W1968590406 @default.
- W2087370523 cites W1974227778 @default.
- W2087370523 cites W1975943401 @default.
- W2087370523 cites W1976656978 @default.
- W2087370523 cites W1977205042 @default.
- W2087370523 cites W1980188152 @default.
- W2087370523 cites W1980983031 @default.
- W2087370523 cites W1982485742 @default.
- W2087370523 cites W1990483261 @default.
- W2087370523 cites W1990975173 @default.
- W2087370523 cites W1991058372 @default.
- W2087370523 cites W1992429037 @default.
- W2087370523 cites W1998181360 @default.
- W2087370523 cites W1998468168 @default.
- W2087370523 cites W2000385831 @default.
- W2087370523 cites W2007186663 @default.
- W2087370523 cites W2010990351 @default.
- W2087370523 cites W2011080806 @default.
- W2087370523 cites W2018294724 @default.
- W2087370523 cites W2018628848 @default.
- W2087370523 cites W2018699213 @default.
- W2087370523 cites W2019014355 @default.
- W2087370523 cites W2024812169 @default.
- W2087370523 cites W2029106442 @default.
- W2087370523 cites W2029211829 @default.
- W2087370523 cites W2032216411 @default.
- W2087370523 cites W2041725617 @default.
- W2087370523 cites W2042661475 @default.
- W2087370523 cites W2052148264 @default.
- W2087370523 cites W2052454212 @default.
- W2087370523 cites W2053214347 @default.
- W2087370523 cites W2054514502 @default.
- W2087370523 cites W2054716545 @default.
- W2087370523 cites W2064175091 @default.
- W2087370523 cites W2065177750 @default.
- W2087370523 cites W2071915484 @default.
- W2087370523 cites W2072900370 @default.
- W2087370523 cites W2077739831 @default.
- W2087370523 cites W2079144517 @default.
- W2087370523 cites W2080171108 @default.
- W2087370523 cites W2080821521 @default.
- W2087370523 cites W2084146839 @default.
- W2087370523 cites W2084708798 @default.
- W2087370523 cites W2086243520 @default.
- W2087370523 cites W2086374051 @default.
- W2087370523 cites W2089435396 @default.
- W2087370523 cites W2089637323 @default.
- W2087370523 cites W2091112973 @default.
- W2087370523 cites W2094014826 @default.
- W2087370523 cites W2106056372 @default.
- W2087370523 cites W2109229908 @default.
- W2087370523 cites W2112500425 @default.
- W2087370523 cites W2136854916 @default.
- W2087370523 cites W2141161103 @default.
- W2087370523 cites W2147138130 @default.
- W2087370523 cites W2403297264 @default.
- W2087370523 cites W2483515871 @default.
- W2087370523 cites W4244753882 @default.
- W2087370523 cites W937100882 @default.
- W2087370523 doi "https://doi.org/10.2116/analsci.17.27" @default.
- W2087370523 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/11993673" @default.
- W2087370523 hasPublicationYear "2001" @default.
- W2087370523 type Work @default.
- W2087370523 sameAs 2087370523 @default.
- W2087370523 citedByCount "16" @default.
- W2087370523 countsByYear W20873705232012 @default.
- W2087370523 countsByYear W20873705232014 @default.
- W2087370523 countsByYear W20873705232015 @default.
- W2087370523 countsByYear W20873705232016 @default.
- W2087370523 countsByYear W20873705232022 @default.
- W2087370523 crossrefType "journal-article" @default.
- W2087370523 hasAuthorship W2087370523A5008356841 @default.
- W2087370523 hasAuthorship W2087370523A5059811057 @default.
- W2087370523 hasAuthorship W2087370523A5074187446 @default.
- W2087370523 hasBestOaLocation W20873705231 @default.
- W2087370523 hasConcept C113196181 @default.
- W2087370523 hasConcept C115537861 @default.
- W2087370523 hasConcept C147789679 @default.
- W2087370523 hasConcept C159985019 @default.
- W2087370523 hasConcept C160756335 @default.
- W2087370523 hasConcept C171250308 @default.
- W2087370523 hasConcept C17525397 @default.
- W2087370523 hasConcept C178790620 @default.
- W2087370523 hasConcept C181199279 @default.
- W2087370523 hasConcept C185592680 @default.
- W2087370523 hasConcept C192562407 @default.
- W2087370523 hasConcept C26771246 @default.