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• W2087373014 abstract "Human monoclonal antibodies (HuMAbs) prepared from patients with viral infections could provide information on human epitopes important for the development of vaccines as well as potential therapeutic applications. Through the fusion of peripheral blood mononuclear cells from a total of five influenza-vaccinated volunteers, with newly developed murine–human chimera fusion partner cells, named SPYMEG, we obtained 10 hybridoma clones stably producing anti-influenza virus antibodies: one for influenza A H1N1, four for influenza A H3N2 and five for influenza B. Surprisingly, most of the HuMAbs showed broad reactivity within subtype and four (two for H3N2 and two for B) showed broad neutralizing ability. Importantly, epitope mapping revealed that the two broad neutralizing antibodies to H3N2 derived from different donors recognized the same epitope located underneath the receptor-binding site of the hemagglutinin globular region that is highly conserved among H3N2 strains." @default.
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• W2087373014 date "2009-09-01" @default.
• W2087373014 modified "2023-10-17" @default.
• W2087373014 title "Broad neutralizing human monoclonal antibodies against influenza virus from vaccinated healthy donors" @default.
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• W2087373014 doi "https://doi.org/10.1016/j.bbrc.2009.06.151" @default.
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