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• W2087391418 abstract "Murine dendritic cells (DC) and macrophages respond to bacterial CpG DNA through toll‐like receptor 9 (TLR9). Although it is frequently assumed that bacterial DNA is a direct stimulus for B cells, published work does not reliably show responses of purified B cells. Here we show that purified splenic B cells did not respond to Escherichia coli DNA with induction of CD86, despite readily responding to single‐stranded (ss) phosphodiester CpG oligodeoxynucleotides (ODN). This was due to a combination of weak responses to both long and double‐stranded (ds) DNA. B‐cell DNA uptake was greatly reduced with increasing DNA length. This contrasts with macrophages where DNA uptake and subsequent responses were enhanced with increasing DNA length. However, when DNA was physically linked to hen egg lysozyme (HEL), HEL‐specific B cells showed efficient uptake of DNA, and limited proliferation in response to the HEL–DNA complex. We propose that, in the absence of other signals, B cells have poor uptake and responses to long dsDNA to prevent polyclonal activation. Conversely, when DNA is physically linked to a B‐cell receptor (BCR) ligand, its uptake is increased, allowing TLR9‐dependent B‐cell activation in an antigen‐specific manner. We could not generate fragments of E. coli DNA by limited DNaseI digestion that could mimic the stimulatory effect of ss CpG ODN on naïve B cells. We suggest that the frequently studied polyclonal B‐cell responses to CpG ODN are relevant to therapeutic applications of phosphorothioate‐modified CpG‐containing ODN, but not to natural responses to foreign or host dsDNA." @default.
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• W2087391418 date "2010-10-05" @default.
• W2087391418 modified "2023-09-26" @default.
• W2087391418 title "B cells do not take up bacterial DNA: an essential role for antigen in exposure of DNA to toll‐like receptor‐9" @default.
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• W2087391418 doi "https://doi.org/10.1038/icb.2010.112" @default.
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