Matches in SemOpenAlex for { <https://semopenalex.org/work/W2087420281> ?p ?o ?g. }
- W2087420281 endingPage "1588" @default.
- W2087420281 startingPage "1588" @default.
- W2087420281 abstract "The free energy provided by adenosine triphosphate (ATP) hydrolysis is central to many cellular processes and, therefore, the number of enzymes utilizing ATP as a substrate is almost innumerable. Modified analogues of ATP are a valuable means to understand the biological function of ATPases. Although these enzymes have evolved towards binding to ATP, large differences in active site architectures were found. In order to systematically access the specific active site constraints of different ATPases suitable tools are required. Here, we present the synthesis of six new ATP-based ATPase probes modified at three different positions of the nucleobase and the ribose, respectively. Subsequently, we studied the ATPases focal adhesion kinase FAK, the ubiquitin-activating protein UBA1 and the kinesin Eg5 as examples for ATPases that process ATP by different mechanisms. We find that for each of these enzymes at least one position in ATP can be modified without loss of acceptance by the enzyme. However, the positions at which modifications are tolerated significantly differ between the studied enzymes allowing fingerprints to be drawn for reactivity. The introduced ATP analogues may form the basis for the design of tailored probes with increased affinity and specificity for a specific ATPase of interest." @default.
- W2087420281 created "2016-06-24" @default.
- W2087420281 creator A5003542465 @default.
- W2087420281 creator A5004861842 @default.
- W2087420281 creator A5009390076 @default.
- W2087420281 creator A5022205362 @default.
- W2087420281 creator A5030213335 @default.
- W2087420281 creator A5035335930 @default.
- W2087420281 creator A5035525469 @default.
- W2087420281 creator A5057465808 @default.
- W2087420281 creator A5065050285 @default.
- W2087420281 date "2013-01-01" @default.
- W2087420281 modified "2023-10-18" @default.
- W2087420281 title "Fingerprinting differential active site constraints of ATPases" @default.
- W2087420281 cites W1597984680 @default.
- W2087420281 cites W1610851002 @default.
- W2087420281 cites W1964020745 @default.
- W2087420281 cites W1965883439 @default.
- W2087420281 cites W1966969980 @default.
- W2087420281 cites W1969342647 @default.
- W2087420281 cites W1970757622 @default.
- W2087420281 cites W1971856698 @default.
- W2087420281 cites W1976806644 @default.
- W2087420281 cites W1979013550 @default.
- W2087420281 cites W1980390493 @default.
- W2087420281 cites W1984133191 @default.
- W2087420281 cites W1988422556 @default.
- W2087420281 cites W1988820070 @default.
- W2087420281 cites W1992796372 @default.
- W2087420281 cites W1996877809 @default.
- W2087420281 cites W2004250476 @default.
- W2087420281 cites W2005585817 @default.
- W2087420281 cites W2008044453 @default.
- W2087420281 cites W2013689413 @default.
- W2087420281 cites W2014280822 @default.
- W2087420281 cites W2017732069 @default.
- W2087420281 cites W2021239932 @default.
- W2087420281 cites W2021502759 @default.
- W2087420281 cites W2027532431 @default.
- W2087420281 cites W2029093937 @default.
- W2087420281 cites W2029178312 @default.
- W2087420281 cites W2030205108 @default.
- W2087420281 cites W2035472375 @default.
- W2087420281 cites W2037172705 @default.
- W2087420281 cites W2040146122 @default.
- W2087420281 cites W2040645831 @default.
- W2087420281 cites W2041727491 @default.
- W2087420281 cites W2043116561 @default.
- W2087420281 cites W2043447781 @default.
- W2087420281 cites W2043716459 @default.
- W2087420281 cites W2044597760 @default.
- W2087420281 cites W2045156702 @default.
- W2087420281 cites W2046984022 @default.
- W2087420281 cites W2049676768 @default.
- W2087420281 cites W2051510417 @default.
- W2087420281 cites W2063603929 @default.
- W2087420281 cites W2065744630 @default.
- W2087420281 cites W2067246673 @default.
- W2087420281 cites W2076194625 @default.
- W2087420281 cites W2085784933 @default.
- W2087420281 cites W2089387712 @default.
- W2087420281 cites W2091516551 @default.
- W2087420281 cites W2095264103 @default.
- W2087420281 cites W2099002508 @default.
- W2087420281 cites W2099393783 @default.
- W2087420281 cites W2099662799 @default.
- W2087420281 cites W2099687412 @default.
- W2087420281 cites W2107449157 @default.
- W2087420281 cites W2108763770 @default.
- W2087420281 cites W2115666559 @default.
- W2087420281 cites W2118030556 @default.
- W2087420281 cites W2123322767 @default.
- W2087420281 cites W2131518505 @default.
- W2087420281 cites W2136133297 @default.
- W2087420281 cites W2140182533 @default.
- W2087420281 cites W2144114582 @default.
- W2087420281 cites W2145419706 @default.
- W2087420281 cites W2148893101 @default.
- W2087420281 cites W2153306606 @default.
- W2087420281 cites W2160168027 @default.
- W2087420281 cites W2166987747 @default.
- W2087420281 cites W2167147371 @default.
- W2087420281 cites W2172047990 @default.
- W2087420281 cites W2327776595 @default.
- W2087420281 cites W2329403794 @default.
- W2087420281 doi "https://doi.org/10.1039/c3sc21916j" @default.
- W2087420281 hasPublicationYear "2013" @default.
- W2087420281 type Work @default.
- W2087420281 sameAs 2087420281 @default.
- W2087420281 citedByCount "20" @default.
- W2087420281 countsByYear W20874202812013 @default.
- W2087420281 countsByYear W20874202812014 @default.
- W2087420281 countsByYear W20874202812015 @default.
- W2087420281 countsByYear W20874202812016 @default.
- W2087420281 countsByYear W20874202812017 @default.
- W2087420281 countsByYear W20874202812018 @default.
- W2087420281 countsByYear W20874202812020 @default.
- W2087420281 countsByYear W20874202812021 @default.