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• W2087456593 abstract "Respiratory chain complex I contains 8–9 iron-sulfur clusters. In several cases, the assignment of these clusters to subunits and binding motifs is still ambiguous. To test the proposed ligation of the tetranuclear iron-sulfur cluster N5 of respiratory chain complex I, we replaced the conserved histidine 129 in the 75-kDa subunit from Yarrowia lipolytica with alanine. In the mutant strain, reduced amounts of fully assembled but destabilized complex I could be detected. Deamino-NADH: ubiquinone oxidoreductase activity was abolished completely by the mutation. However, EPR spectroscopic analysis of mutant complex I exhibited an unchanged cluster N5 signal, excluding histidine 129 as a cluster N5 ligand. Respiratory chain complex I contains 8–9 iron-sulfur clusters. In several cases, the assignment of these clusters to subunits and binding motifs is still ambiguous. To test the proposed ligation of the tetranuclear iron-sulfur cluster N5 of respiratory chain complex I, we replaced the conserved histidine 129 in the 75-kDa subunit from Yarrowia lipolytica with alanine. In the mutant strain, reduced amounts of fully assembled but destabilized complex I could be detected. Deamino-NADH: ubiquinone oxidoreductase activity was abolished completely by the mutation. However, EPR spectroscopic analysis of mutant complex I exhibited an unchanged cluster N5 signal, excluding histidine 129 as a cluster N5 ligand. Respiratory chain complex I links the transfer of two electrons from NADH to ubiquinone to the translocation of four protons across the respiratory membrane (1Brandt U. Kerscher S. Dro ̈se S. Zwicker K. Zickermann V. FEBS Lett. 2003; 545: 9-17Crossref PubMed Scopus (125) Google Scholar). Only low resolution structures of complex I are available, demonstrating that the mitochondrial enzyme is L-shaped and consists of a membrane arm and a peripheral arm (2Guenebaut V. Schlitt A. Weiss H. Leonard K. Friedrich T. J. Mol. Biol. 1998; 276: 105-112Crossref PubMed Scopus (204) Google Scholar, 3Grigorieff N. J. Mol. Biol. 1998; 277: 1033-1046Crossref PubMed Scopus (299) Google Scholar, 4Djafarzadeh R. Kerscher S. Zwicker K. Radermacher M. Lindahl M. Scha ̈gger H. Brandt U. Biochim. Biophys. Acta. 2000; 1459: 230-238Crossref PubMed Scopus (82) Google Scholar). The reaction mechanism is largely unknown. Electrons from NADH are first transferred to FMN, non-covalently bound to the 51-kDa subunit, then to a series of iron-sulfur clusters in the peripheral arm, and finally to the ubiquinone reduction site which, as has been demonstrated by site-directed mutagenesis in the obligate aerobic yeast Yarrowia lipolytica, is partly contained in the 49-kDa subunit of the peripheral arm (5Kashani-Poor N. Zwicker K. Kerscher S. Brandt U. J. Biol. Chem. 2001; 276: 24082-24087Abstract Full Text Full Text PDF PubMed Scopus (125) Google Scholar). Localization by electron microscopic single particle analysis of two anti-49-kDa subunit monoclonal antibody epitopes has provided evidence that this catalytic core of complex I is located ∼70–80 Å away from the phospholipid bilayer (6Zickermann V. Bostina M. Hunte C. Ruiz T. Radermacher M. Brandt U. J. Biol. Chem. 2003; 278: 29072-29078Abstract Full Text Full Text PDF PubMed Scopus (73) Google Scholar). From these and other results, an indirect coupling mechanism between quinone reduction in the peripheral arm and proton pumping in the membrane arm seems likely (1Brandt U. Kerscher S. Dro ̈se S. Zwicker K. Zickermann V. FEBS Lett. 2003; 545: 9-17Crossref PubMed Scopus (125) Google Scholar, 7Friedrich T. J. Bioenerg. Biomembr. 2001; 33: 169-177Crossref PubMed Scopus (148) Google Scholar, 8Yagi T. Matsuno-Yagi A. Biochemistry. 2003; 42: 2266-2274Crossref PubMed Scopus (265) Google Scholar). Little information is available on the spatial arrangement of the other components of the electron transfer pathway. EPR 1The abbreviations used are: EPR, electron paramagnetic resonance; BN, blue native; HAR, hexa-ammine-ruthenium(III)-chloride.-detectable iron-sulfur clusters have been assigned to individual subunits (9Ohnishi T. Biochim. Biophys. Acta. 1998; 1364: 186-206Crossref PubMed Scopus (384) Google Scholar) as follows: the 75-kDa subunit, containing the [Fe2S2] cluster N1b and the [Fe4S4] clusters N4 and N5; the 51-kDa subunit, containing [Fe4S4] cluster N3; the 24-kDa subunit, containing the [Fe2S2] cluster N1a; and the PSST subunit, containing the [Fe4S4] cluster N2. Two EPR-silent [Fe4S4] clusters, termed N6a and N6b, have been assigned to the ferredoxin-like subunit of Neurospora crassa complex I homologous to bovine TYKY (10Rasmussen T. Scheide D. Brors B. Kintscher L. Weiss H. Friedrich T. Biochemistry. 2001; 40: 6124-6131Crossref PubMed Scopus (67) Google Scholar). The tetranuclear iron-sulfur cluster N5 is only observed at very low temperatures and high microwave power. After its initial discovery in pigeon heart complex I (11Ohnishi T. Salerno J.C. Winter D.B. Lim J. Yu C.-A. Yu L. King T.E. Biochim. Biophys. Acta. 1975; 387: 475-490Crossref PubMed Scopus (72) Google Scholar), it has also been identified in bovine (9Ohnishi T. Biochim. Biophys. Acta. 1998; 1364: 186-206Crossref PubMed Scopus (384) Google Scholar), Rhodobacter spaeroides (12Sled V.D. Friedrich T. Leif H. Weiss H. Fukumori Y. Calhoun M.W. Gennis R.B. Ohnishi T. Meinhardt S.W. J. Bioenerg. Biomembr. 1993; 25: 347-356Crossref PubMed Scopus (84) Google Scholar), and Y. lipolytica (4Djafarzadeh R. Kerscher S. Zwicker K. Radermacher M. Lindahl M. Scha ̈gger H. Brandt U. Biochim. Biophys. Acta. 2000; 1459: 230-238Crossref PubMed Scopus (82) Google Scholar, 13Kerscher S. Kashani-Poor N. Zwicker K. Zickermann V. Brandt U. J. Bioenerg. Biomembr. 2001; 33: 187-196Crossref PubMed Scopus (45) Google Scholar), but not in N. crassa (14Weiss H. Friedrich T. Hofhaus G. Preis D. Eur. J. Biochem. 1991; 197: 563-576Crossref PubMed Scopus (427) Google Scholar) complex I. In Y. lipolytica, cluster N5 shows very fast spin relaxation with a half-saturation parameter of ∼180 milliwatts at 5 K. The line width of the gz signal (Lz) is in the range of 2.5–3 millitesla. Similar to observations in other model systems, the spin concentration of cluster N5 is much lower than that of the other EPR-detectable clusters (13Kerscher S. Kashani-Poor N. Zwicker K. Zickermann V. Brandt U. J. Bioenerg. Biomembr. 2001; 33: 187-196Crossref PubMed Scopus (45) Google Scholar). The N-terminal part of the 75-kDa subunit contains three highly conserved iron-sulfur cluster motifs and is homologous to the N-terminal portion of iron-only hydrogenases (15Smith M.A. Finel M. Korolik V. Mendz G.L. Arch. Microbiol. 2000; 174: 1-10Crossref PubMed Scopus (77) Google Scholar). In the x-ray structure of the iron-only hydrogenase from CpI of Clostridium pasteurianum, these motifs ligate one [Fe2S2] and two [Fe4S4] iron-sulfur clusters (16Peters J.W. Lanzilotta W.N. Lemon B.J. Seefeldt L.C. Science. 1998; 282: 1853-1858Crossref PubMed Google Scholar). Recently, direct evidence for the presence of three iron-sulfur clusters in the heterologously expressed 75-kDa subunit from Paracoccus denitrificans has been presented (17Yano T. Sklar J. Nakamaru-Ogiso E. Yagi T. Ohnishi T. J. Biol. Chem. 2003; 278: 15514-15522Abstract Full Text Full Text PDF PubMed Scopus (45) Google Scholar). In the same study, the fast relaxing [Fe4S4] cluster N5 has been assigned to the second binding motif of the 75-kDa subunit, an unusual HXXXCXXCXXXXXC motif that contains three cysteines and a histidine. Secondary structure predictions using the PROF algorithm (26Ouali M. King R.D. Protein Sci. 2000; 9: 1162-1176Crossref PubMed Scopus (299) Google Scholar) suggest that this sequence element, both in hydrogenases and complex I from various organisms, forms a loop bounded by α-helices (Fig. 1). To test the proposed assignment of this sequence as a cluster N5 ligation motif in fully assembled complex I, we mutated the conserved histidine 129 of the Y. lipolytica 75-kDa subunit to alanine. The Y. lipolytica deletion strain nuamΔ (ura3–302, leu2-270, lys11-23, nuam::URA3, NUGM-Htg2, NDH2i, MatB), in which a 1.7-kb ClaI/KpnI fragment corresponding to codons 34–598 of the NUAM gene encoding the 728-amino acid precursor of the 75-kDa subunit of complex I is replaced with the Y. lipolytica URA3 gene (1.6 kb) oriented in opposite direction to the original NUAM open reading frame, was constructed by the double homologous recombination strategy published previously (18Kerscher S. Dro ̈se S. Zwicker K. Zickermann V. Brandt U. Biochim. Biophys. Acta. 2002; 1555: 83-91Crossref PubMed Scopus (87) Google Scholar). A wild type and a PCR-generated H129A mutant version of the NUAM gene were then subcloned as 5.2-kb SalI fragments (after an internal SalI site in the NUAM open reading frame had been mutagenized to GTTGAC) into the replicative plasmid pUB4 (19Kerscher S. Eschemann A. Okun P.M. Brandt U. J. Cell Sci. 2001; 114: 3915-3921Crossref PubMed Google Scholar) and transformed into strain nuamΔ, resulting in the strains nuamΔ, pNUAM-wild type (parental) and nuamΔ, pNUAM-H129A (mutant). The presence of the point mutation in large scale cultures of strain nuamΔ, pNUAM-H129A was verified by the isolation of total DNA from 5-ml aliquots and the direct sequencing of PCR products. Complex I catalytic activities in Y. lipolytica mitochondrial membranes were measured as deamino-NADH:n-decylubiquinone (DBQ) or NADH:HAR catalytic rates as described (20Kerscher S. Be ́nit P. Abdrakhmanova A. Zwicker K. Rais I. Karas M. Rustin P. Brandt U. Eur. J. Biochem. 2004; 271: 3588-3595Crossref PubMed Scopus (9) Google Scholar). A BN-PAGE (21Scha ̈gger H. Hunte C. von Jagow G. Scha ̈gger H. Membrane Protein Purification and Crystallization: A Practical Guide. Academic Press, San Diego, CA2003: 105-130Crossref Google Scholar) of mitochondrial membranes was performed using 4–16% gradient gels. A His-tagged complex I was isolated by the extraction of mitochondrial membranes with dodecyl maltoside followed by Ni2+ affinity and size exclusion chromatography as published previously (22Kashani-Poor N. Kerscher S. Zickermann V. Brandt U. Biochim. Biophys. Acta. 2001; 1504: 363-370Crossref PubMed Scopus (74) Google Scholar). Low temperature EPR spectra were obtained on a Bruker ESP 300E spectrometer equipped with a liquid helium continuous flow cryostat, ESR 900, from Oxford instruments. 150-μl samples were reduced with 1 mm NADH. As a qualitative test for complex I assembly, mitochondrial membranes from strain nuamΔ, pNUAM-H129A were analyzed by BN-PAGE. As illustrated in Fig. 2, a fully assembled enzyme appeared to be present in the mutant strain, but the intensities of all subunit bands were strongly reduced compared with those in the parental strain. In contrast, electron transfer from NADH to the artificial acceptor HAR (23Gavrikova E.V. Grivennikova V.G. Sled V.D. Ohnishi T. Vinogradov A.D. Biochim. Biophys. Acta. 1995; 1230: 23-30Crossref PubMed Scopus (35) Google Scholar) was only slightly reduced in mitochondrial membranes from strain nuamΔ, pNUAM-H129A and still amounted to 74% of the parental strain value (Table I). This result could be explained by the presence of subcomplexes in the membranes from this mutant; because FMN bound to the 51-kDa subunit is sufficient to catalyze the non-physiological reaction, subcomplexes containing this subunit can show NADH:HAR activity. These subcomplexes may have escaped detection by BN-PAGE because of their instability. At any rate, it was clear that the H129A mutation had markedly reduced complex I stability. It should be noted that significant residual NADH:HAR activity was also found in the nuamΔ strain carrying the empty plasmid pUB4 (Table I), although no assembled complex I was detectable by BN-PAGE in this strain (not shown). We also mutated several cysteines in all three iron-sulfur binding motifs of the 75-kDa subunit to alanines, but in all cases we failed to detect complex I assembly or NADH:HAR activity above the level observed in strain nuamΔ, pUB4 (data not shown).Table INADH:HAR and dNADH:DBQ activities of mitochondrial membranesStrainNADH:HAR activitydNADH:DBQ activityμmol min-1 mg-1Percent of activityμmol min-1 mg-1Percent of activityNuamΔ,pNUAM-WT0.841000.290100NuamΔ,pNUAM-H129A0.6274<0.01<3NuamΔ,pUB40.2833<0.01<3 Open table in a new tab Specific deamino-NADH:n-decylubiquinone activity of membranes from strain nuamΔ, pNUAM-H129A was below detection level. This finding demonstrated that even the fully assembled fraction of complex I (Fig. 2) in the mitochondrial membranes lacked ubiquinone reductase activity. The mutant strain exhibited very low growth yields in complete media (14 g/liter wet weight, i.e. 4–6 times less than that commonly observed for the parental strain). This result is remarkable, as strain nuamΔ, which lacked most of the open reading frame for the 75-kDa subunit, grew normally. The reason for this growth phenotype is unknown. Complex I from strain nuamΔ, pNUAM-H129A was purified by Ni2+ affinity and gel filtration chromatography (22Kashani-Poor N. Kerscher S. Zickermann V. Brandt U. Biochim. Biophys. Acta. 2001; 1504: 363-370Crossref PubMed Scopus (74) Google Scholar). Mitochondrial membranes equivalent to 4.5 grams of total protein were used for a complex I preparation, which gave 1.5 mg of purified enzyme. This was <10% of the yield typically obtained from the parental strain, suggesting that a considerable portion of assembled complex I had dissociated during the purification procedure. This finding supports the notion that the mutation H129A had destabilized complex I. EPR spectroscopy was performed with complex I from strain nuamΔ, pNUAM-H129A and from the parental strain. At a temperature of 40 K and a microwave power of 1 milliwatt, a single binuclear cluster called N1 could be detected (Fig. 3A). At a temperature of 12 K and a microwave power of 1 milliwatt, signals arising from clusters N1, N2, N3, and N4 could be seen (Fig. 3B). Cluster N5 signals were detected at 5 K and a microwave power of 100 milliwatts (Fig. 3C). Confirming previously published results (13Kerscher S. Kashani-Poor N. Zwicker K. Zickermann V. Brandt U. J. Bioenerg. Biomembr. 2001; 33: 187-196Crossref PubMed Scopus (45) Google Scholar), the g values of cluster N5 from wild-type Y. lipolytica complex I were gz,y,x = 2.062, 1.93, and ∼1.89. The gx and gy signals of cluster N5 were difficult to assign, because they overlapped with the gx and gy signals of clusters N1 and even more with N4. Interference by the N4 signal also was much more severe, as this cluster was not completely power saturated under the EPR conditions used. However, the gz signal of cluster N5 was clearly separated from any other EPR signal in isolated complex I from Y. lipolytica. The spectra shown in Fig. 3C clearly show that the cluster N5 gz signals of both preparations were virtually identical in terms of peak intensity, peak width, and field position (Fig. 3C). This result indicated that cluster N5 was still present in complex I from strain nuamΔ, pNUAM-H129A and that its geometry was unaffected. Virtually identical results were obtained in two independent preparations of the mutant enzyme. We have replaced a strictly conserved histidine residue (His-129) in the 75-kDa subunit of Y. lipolytica complex I with alanine to test whether this residue is a ligand of iron-sulfur cluster N5, as has been proposed recently (17Yano T. Sklar J. Nakamaru-Ogiso E. Yagi T. Ohnishi T. J. Biol. Chem. 2003; 278: 15514-15522Abstract Full Text Full Text PDF PubMed Scopus (45) Google Scholar). As the EPR signature of cluster N5 was completely unchanged in complex I purified from the mutant strain, we could exclude this possibility. Our results are in contradiction with a recent study with a heterologously expressed 75-kDa subunit from P. denitrificans (17Yano T. Sklar J. Nakamaru-Ogiso E. Yagi T. Ohnishi T. J. Biol. Chem. 2003; 278: 15514-15522Abstract Full Text Full Text PDF PubMed Scopus (45) Google Scholar). From changes in the EPR spectrum of the iron-sulfur cluster N5 that were caused by a mutation changing the corresponding histidine to a cysteine, the authors concluded that the HXXXCXXCXXXXXC motif of the 75-kDa subunit binds cluster N5. The most obvious explanation for the results obtained with the P. denitrificans H106C mutant would be that structural changes had indirectly changed the geometry of cluster N5. Such EPR signal reduction, peak broadening, and shifting effects have been described, e.g. for point mutations affecting amino acids in the vicinity of cluster N2 of complex I from Y. lipolytica (5Kashani-Poor N. Zwicker K. Kerscher S. Brandt U. J. Biol. Chem. 2001; 276: 24082-24087Abstract Full Text Full Text PDF PubMed Scopus (125) Google Scholar, 24Ahlers P. Zwicker K. Kerscher S. Brandt U. J. Biol. Chem. 2000; 275: 23577-23582Abstract Full Text Full Text PDF PubMed Scopus (67) Google Scholar, 25Garofano A. Zwicker K. Kerscher S. Okun P. Brandt U. J. Biol. Chem. 2003; 278: 42435-42440Abstract Full Text Full Text PDF PubMed Scopus (41) Google Scholar), and can be expected to be more pronounced when single subunits instead of completely assembled multi-subunit complexes are studied. In fact, significant instability of the mutant P. dentrificans 75-kDa subunit was noted (17Yano T. Sklar J. Nakamaru-Ogiso E. Yagi T. Ohnishi T. J. Biol. Chem. 2003; 278: 15514-15522Abstract Full Text Full Text PDF PubMed Scopus (45) Google Scholar). Although we also observed destabilization of Y. lipolytica complex I by the H129A mutation, it obviously did not affect the EPR spectra of the purified enzyme. A more fundamental problem with complex I from P. denitrificans is that the g signal values of cluster N5, detected at 5 K, are very close to those of cluster N4, detected at 12 K (17Yano T. Sklar J. Nakamaru-Ogiso E. Yagi T. Ohnishi T. J. Biol. Chem. 2003; 278: 15514-15522Abstract Full Text Full Text PDF PubMed Scopus (45) Google Scholar). Distinction between these clusters relies on the assumption that the cluster N4 signals of P. denitrificans complex I are completely power saturated at 5 K and a microwave power of 100 milliwatt, which in our hands is clearly not the case for cluster N4 of Bos taurus and Y. lipolytica complex I. Therefore, although at least the gz signals of clusters N4 and N5 are well separated in the latter two model organisms, the assignment of EPR signals to cluster N5 of P. denitrificans complex I is more difficult. It should be noted that the cluster N4 EPR signals were also found to be somewhat affected by the H106C mutation in P. denitrificans complex I (17Yano T. Sklar J. Nakamaru-Ogiso E. Yagi T. Ohnishi T. J. Biol. Chem. 2003; 278: 15514-15522Abstract Full Text Full Text PDF PubMed Scopus (45) Google Scholar). Thus, there is another possibility in regard to how the findings in P. denitrificans and Y. lipolytica could be reconciled. If the iron-sulfur cluster assignment within the 75-kDa subunit was revised, the mutation of the conserved histidine in Y. lipolytica may have resulted in the removal of a cluster N1b ligand, leaving the EPR signature of cluster N5 unchanged. The histidine to cysteine exchange in P. denitrificans as such may then have been EPR-silent, but it may have caused a structural alteration that affected the EPR patterns of both clusters N4 and N5. However, because the study by Yano et al. (17Yano T. Sklar J. Nakamaru-Ogiso E. Yagi T. Ohnishi T. J. Biol. Chem. 2003; 278: 15514-15522Abstract Full Text Full Text PDF PubMed Scopus (45) Google Scholar) clearly demonstrates the presence of three iron-sulfur clusters in the heterologously expressed 75-kDa subunit of P. denitrificans, this proposal raises another problem. If all three motifs in the 75-kDa hold an iron-sulfur cluster, why did the H129A mutation not have any effect on the EPR spectra of Y. lipolytica complex I? Possible answers are that cluster N1b is either not present or undetectable by standard EPR spectroscopy in complex I from Y. lipolytica. Although complex I from bovine heart mitochondria and various other sources is known to contain two EPR detectable binuclear iron-sulfur clusters called N1a and N1b (9Ohnishi T. Biochim. Biophys. Acta. 1998; 1364: 186-206Crossref PubMed Scopus (384) Google Scholar), only one binuclear iron-sulfur cluster (called N1) could be detected in complex I from Y. lipolytica to date. The stoichiometry of clusters N1/N2/N3/N4 was determined as 1:1: 1:1 (13Kerscher S. Kashani-Poor N. Zwicker K. Zickermann V. Brandt U. J. Bioenerg. Biomembr. 2001; 33: 187-196Crossref PubMed Scopus (45) Google Scholar). As the N1 signal of Y. lipolytica complex I was not detectable in a subcomplex 2V. Zickermann, K. Zwicker, M. Bostina, M. Radermacher, and U. Brandt, manuscript in preparation. including the 75-kDa but lacking the 24-kDa subunit, which in bovine heart complex I contains binuclear cluster N1a, it seems tempting to speculate that also in the Y. lipolytica enzyme the 75-kDa subunit may carry a second binuclear cluster that has, to date, escaped detection by EPR. If the HXXXCXXCXXXXXC motif ligated this EPR silent cluster, it would have been removed by the H129A mutation, offering a straightforward explanation of why ubiquinone reductase activity was lost. As a consequence, one has to conclude that in complex I the use of the iron-sulfur binding motifs has changed from iron-only hydrogenases. Either the histidine in question that is conserved in all known complex I sequences has acquired a different function in complex I, or the motif ligating a binuclear cluster in hydrogenase binds a tetranuclear cluster (N5) in complex I and vice versa. We thank Gudrun Beyer for excellent technical assistance and Nikki Bolcevic for help in the isolation of strain nuamΔ. We thank Maja Aleksandra Tocilescu for preparing the BN-PAGE gels shown in Fig. 2 and Hermann Schägger for help with interpretation of the gels." @default.
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• W2087456593 title "Histidine 129 in the 75-kDa Subunit of Mitochondrial Complex I from Yarrowia lipolytica Is Not a Ligand for [Fe4S4] Cluster N5 but Is Required for Catalytic Activity" @default.
• W2087456593 cites W176612975 @default.
• W2087456593 cites W1968229549 @default.
• W2087456593 cites W1968315028 @default.
• W2087456593 cites W1970274107 @default.
• W2087456593 cites W1974665653 @default.
• W2087456593 cites W2001063971 @default.
• W2087456593 cites W2001077031 @default.
• W2087456593 cites W2005969857 @default.
• W2087456593 cites W2016998801 @default.
• W2087456593 cites W2019356464 @default.
• W2087456593 cites W2020969870 @default.
• W2087456593 cites W2039386063 @default.
• W2087456593 cites W2050041499 @default.
• W2087456593 cites W2050574751 @default.
• W2087456593 cites W2053621743 @default.
• W2087456593 cites W2058423587 @default.
• W2087456593 cites W2075137223 @default.
• W2087456593 cites W2075704767 @default.
• W2087456593 cites W2078018118 @default.
• W2087456593 cites W2090192950 @default.
• W2087456593 cites W2104796556 @default.
• W2087456593 cites W2111478029 @default.
• W2087456593 cites W2133205270 @default.
• W2087456593 cites W2413672883 @default.
• W2087456593 cites W54644185 @default.
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