FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2087468051> ?p ?o ?g. }

• W2087468051 endingPage "32762" @default.
• W2087468051 startingPage "32753" @default.
• W2087468051 abstract "Old Yellow Enzyme (OYE) binds phenolic ligands forming long wavelength (500–800 nm) charge-transfer complexes. The enzyme is reduced by NADPH, and oxygen, quinones, and α,β-unsaturated aldehydes and ketones can act as electron acceptors to complete catalytic turnover. Solution of the crystal structure of OYE1 from brewer's bottom yeast (Fox, K. M., and Karplus, P. A. (1994) Structure 2, 1089–1105) made it possible to identify histidine 191 and asparagine 194 as amino acid residues that hydrogen-bond with the phenolic ligands, stabilizing the anionic form involved in charge-transfer interaction with the FMN prosthetic group. His-191 and Asn-194 are also predicted to interact with the nicotinamide ring of NADPH in the active site. Mutations of His-191 to Asn, Asn-194 to His, and a double mutation, H191N/N194H, were made of OYE1. It was not possible to isolate the N191H mutant enzyme, but the other two mutant forms had the expected effect on phenolic ligand binding, i.e. decreased binding affinity and decreased charge-transfer absorbance. Reduction of the H191N mutant enzyme by NADPH was similar to that of OYE1, but the reduction rate constant for NADH was greatly decreased. The double mutant enzyme had an increased rate constant for reduction by NADPH, but the reduction rate constant with NADH was lower by a factor of 15. The reactivity of OYE1 and the mutant enzymes with oxygen was similar, but the reactivity of 2-cyclohexenone was greatly decreased by the mutations. The crystal structures of the two mutant forms showed only minor changes from that of the wild type enzyme." @default.
• W2087468051 created "2016-06-24" @default.
• W2087468051 creator A5027076586 @default.
• W2087468051 creator A5052473721 @default.
• W2087468051 creator A5079221179 @default.
• W2087468051 creator A5086284575 @default.
• W2087468051 date "1998-12-01" @default.
• W2087468051 modified "2023-09-28" @default.
• W2087468051 title "On the Active Site of Old Yellow Enzyme" @default.
• W2087468051 cites W130271162 @default.
• W2087468051 cites W1511190700 @default.
• W2087468051 cites W1511510926 @default.
• W2087468051 cites W1517316106 @default.
• W2087468051 cites W1557412251 @default.
• W2087468051 cites W1561680536 @default.
• W2087468051 cites W1602868925 @default.
• W2087468051 cites W1819058188 @default.
• W2087468051 cites W1819423303 @default.
• W2087468051 cites W189861307 @default.
• W2087468051 cites W1945782561 @default.
• W2087468051 cites W1973425278 @default.
• W2087468051 cites W1978096114 @default.
• W2087468051 cites W1986735302 @default.
• W2087468051 cites W1996607909 @default.
• W2087468051 cites W2001382343 @default.
• W2087468051 cites W2003326305 @default.
• W2087468051 cites W2016284676 @default.
• W2087468051 cites W2024619290 @default.
• W2087468051 cites W2034409002 @default.
• W2087468051 cites W2043400750 @default.
• W2087468051 cites W2055043387 @default.
• W2087468051 cites W2058044050 @default.
• W2087468051 cites W2063865658 @default.
• W2087468051 cites W2069424964 @default.
• W2087468051 cites W2082184570 @default.
• W2087468051 cites W2117785785 @default.
• W2087468051 cites W2159642717 @default.
• W2087468051 cites W2168848189 @default.
• W2087468051 cites W294420646 @default.
• W2087468051 doi "https://doi.org/10.1074/jbc.273.49.32753" @default.
• W2087468051 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/9830019" @default.
• W2087468051 hasPublicationYear "1998" @default.
• W2087468051 type Work @default.
• W2087468051 sameAs 2087468051 @default.
• W2087468051 citedByCount "119" @default.
• W2087468051 countsByYear W20874680512012 @default.
• W2087468051 countsByYear W20874680512013 @default.
• W2087468051 countsByYear W20874680512014 @default.
• W2087468051 countsByYear W20874680512015 @default.
• W2087468051 countsByYear W20874680512016 @default.
• W2087468051 countsByYear W20874680512017 @default.
• W2087468051 countsByYear W20874680512018 @default.
• W2087468051 countsByYear W20874680512019 @default.
• W2087468051 countsByYear W20874680512020 @default.
• W2087468051 countsByYear W20874680512021 @default.
• W2087468051 countsByYear W20874680512022 @default.
• W2087468051 countsByYear W20874680512023 @default.
• W2087468051 crossrefType "journal-article" @default.
• W2087468051 hasAuthorship W2087468051A5027076586 @default.
• W2087468051 hasAuthorship W2087468051A5052473721 @default.
• W2087468051 hasAuthorship W2087468051A5079221179 @default.
• W2087468051 hasAuthorship W2087468051A5086284575 @default.
• W2087468051 hasBestOaLocation W20874680511 @default.
• W2087468051 hasConcept C104317684 @default.
• W2087468051 hasConcept C121332964 @default.
• W2087468051 hasConcept C143065580 @default.
• W2087468051 hasConcept C148898269 @default.
• W2087468051 hasConcept C181199279 @default.
• W2087468051 hasConcept C185592680 @default.
• W2087468051 hasConcept C2775895851 @default.
• W2087468051 hasConcept C2778460671 @default.
• W2087468051 hasConcept C33093398 @default.
• W2087468051 hasConcept C41183919 @default.
• W2087468051 hasConcept C55493867 @default.
• W2087468051 hasConcept C62520636 @default.
• W2087468051 hasConcept C71240020 @default.
• W2087468051 hasConcept C93391505 @default.
• W2087468051 hasConceptScore W2087468051C104317684 @default.
• W2087468051 hasConceptScore W2087468051C121332964 @default.
• W2087468051 hasConceptScore W2087468051C143065580 @default.
• W2087468051 hasConceptScore W2087468051C148898269 @default.
• W2087468051 hasConceptScore W2087468051C181199279 @default.
• W2087468051 hasConceptScore W2087468051C185592680 @default.
• W2087468051 hasConceptScore W2087468051C2775895851 @default.
• W2087468051 hasConceptScore W2087468051C2778460671 @default.
• W2087468051 hasConceptScore W2087468051C33093398 @default.
• W2087468051 hasConceptScore W2087468051C41183919 @default.
• W2087468051 hasConceptScore W2087468051C55493867 @default.
• W2087468051 hasConceptScore W2087468051C62520636 @default.
• W2087468051 hasConceptScore W2087468051C71240020 @default.
• W2087468051 hasConceptScore W2087468051C93391505 @default.
• W2087468051 hasIssue "49" @default.
• W2087468051 hasLocation W20874680511 @default.
• W2087468051 hasOpenAccess W2087468051 @default.
• W2087468051 hasPrimaryLocation W20874680511 @default.
• W2087468051 hasRelatedWork W1967826008 @default.
• W2087468051 hasRelatedWork W1999275302 @default.
• W2087468051 hasRelatedWork W2044320339 @default.

• next