FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2087478118> ?p ?o ?g. }

• W2087478118 endingPage "537" @default.
• W2087478118 startingPage "529" @default.
• W2087478118 abstract "Certain tumor cells, such as squamous carcinomas and gliomas, can have an increased number of epidermal-growth-factor (EGF) receptors. The EGF receptors can in these cases be targets for toxic conjugates with specific binding. EGF-based toxic conjugates are potential targeting agents. We have analyzed the internalization and excretion of 125I administered in the form of 125I-EGF-dextran in squamous-carcinoma A431 cells. 125I-EGF without dextran was used for comparison. A431 cells have large numbers of EGF receptors and are capable both of recycling and of degradation of internalized receptor-ligand complexes. The binding of 125I-EGF-dextran and 125I-EGF was receptor-specific, since both ligands competed with non-radioactive EGF for binding. The amount of internalized 125I as a function of time increased continuously within 24 hr following administration of radioactivity as 125I-EGF-dextran. The time pattern was quite different when 125I-EGF without dextran was applied. In the latter case, the amount of internalized radioactivity decreased already after a few hours, probably depending on degradation of EGF. Pre-incubation of the cells with 125I-EGF-dextran or 125I-EGF and analysis of retained and released 125I activity at different times after washing showed that the 125I activity was retained for longer periods of time when EGF-dextran was used instead of EGF. About 30% of the internalized 125I activity was retained after 24 hr when EGF-dextran was used, compared with excretion of nearly all the radioactivity within 5 hr when EGF was used. In some experiments a high concentration of non-radioactive EGF, 5 micrograms/ml, was given to the cells after pre-incubation with 125I-EGF-dextran. This changed the retention and excretion patterns, so that a larger amount of 125I was excreted in the macromolecular fraction and a smaller amount of 125I activity was retained in the cells. Gel chromatography of the 125I activity released into the culture medium showed that the variations in molecular weight were larger after administration of a high concentration of non-radioactive EGF, most likely due to partial degradation of EGF-dextran. The results regarding excretion are in conformity with a model of competition between recycling of EGF-dextran-EGF-receptor complexes and trapping of EGF-dextran in the lysosomes followed by slow degradation. For targeting purposes, it is worth noting that the radioactivity administered in the form of 125I-EGF-dextran had a longer retention time than when 125I-EGF without dextran was used, and that the retention and excretion rates could be modified by post-treatment with the receptor ligand itself." @default.
• W2087478118 created "2016-06-24" @default.
• W2087478118 creator A5040034652 @default.
• W2087478118 creator A5066829495 @default.
• W2087478118 creator A5086311366 @default.
• W2087478118 date "1994-02-15" @default.
• W2087478118 modified "2023-09-27" @default.
• W2087478118 title "Internalization and excretion of epidermal growth factor-dextran-associated radioactivity in cultured human squamous-carcinoma cells" @default.
• W2087478118 cites W1553513611 @default.
• W2087478118 cites W1974205578 @default.
• W2087478118 cites W1975459695 @default.
• W2087478118 cites W1985749328 @default.
• W2087478118 cites W2010769047 @default.
• W2087478118 cites W2012635088 @default.
• W2087478118 cites W2020475637 @default.
• W2087478118 cites W2023626809 @default.
• W2087478118 cites W2031038882 @default.
• W2087478118 cites W2040469480 @default.
• W2087478118 cites W2042652729 @default.
• W2087478118 cites W2078342447 @default.
• W2087478118 cites W2080711144 @default.
• W2087478118 cites W2084072745 @default.
• W2087478118 cites W2093269773 @default.
• W2087478118 cites W2133809169 @default.
• W2087478118 cites W2143048662 @default.
• W2087478118 cites W2152780905 @default.
• W2087478118 cites W2170199119 @default.
• W2087478118 cites W2294921881 @default.
• W2087478118 cites W2316435096 @default.
• W2087478118 doi "https://doi.org/10.1002/ijc.2910560412" @default.
• W2087478118 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/7509322" @default.
• W2087478118 hasPublicationYear "1994" @default.
• W2087478118 type Work @default.
• W2087478118 sameAs 2087478118 @default.
• W2087478118 citedByCount "22" @default.
• W2087478118 countsByYear W20874781182016 @default.
• W2087478118 crossrefType "journal-article" @default.
• W2087478118 hasAuthorship W2087478118A5040034652 @default.
• W2087478118 hasAuthorship W2087478118A5066829495 @default.
• W2087478118 hasAuthorship W2087478118A5086311366 @default.
• W2087478118 hasConcept C10146269 @default.
• W2087478118 hasConcept C126322002 @default.
• W2087478118 hasConcept C134018914 @default.
• W2087478118 hasConcept C139770010 @default.
• W2087478118 hasConcept C170493617 @default.
• W2087478118 hasConcept C185592680 @default.
• W2087478118 hasConcept C190283241 @default.
• W2087478118 hasConcept C2776362946 @default.
• W2087478118 hasConcept C2777339698 @default.
• W2087478118 hasConcept C2777546739 @default.
• W2087478118 hasConcept C29537977 @default.
• W2087478118 hasConcept C2994491419 @default.
• W2087478118 hasConcept C47170353 @default.
• W2087478118 hasConcept C55493867 @default.
• W2087478118 hasConcept C67082663 @default.
• W2087478118 hasConcept C71924100 @default.
• W2087478118 hasConcept C86803240 @default.
• W2087478118 hasConceptScore W2087478118C10146269 @default.
• W2087478118 hasConceptScore W2087478118C126322002 @default.
• W2087478118 hasConceptScore W2087478118C134018914 @default.
• W2087478118 hasConceptScore W2087478118C139770010 @default.
• W2087478118 hasConceptScore W2087478118C170493617 @default.
• W2087478118 hasConceptScore W2087478118C185592680 @default.
• W2087478118 hasConceptScore W2087478118C190283241 @default.
• W2087478118 hasConceptScore W2087478118C2776362946 @default.
• W2087478118 hasConceptScore W2087478118C2777339698 @default.
• W2087478118 hasConceptScore W2087478118C2777546739 @default.
• W2087478118 hasConceptScore W2087478118C29537977 @default.
• W2087478118 hasConceptScore W2087478118C2994491419 @default.
• W2087478118 hasConceptScore W2087478118C47170353 @default.
• W2087478118 hasConceptScore W2087478118C55493867 @default.
• W2087478118 hasConceptScore W2087478118C67082663 @default.
• W2087478118 hasConceptScore W2087478118C71924100 @default.
• W2087478118 hasConceptScore W2087478118C86803240 @default.
• W2087478118 hasIssue "4" @default.
• W2087478118 hasLocation W20874781181 @default.
• W2087478118 hasLocation W20874781182 @default.
• W2087478118 hasOpenAccess W2087478118 @default.
• W2087478118 hasPrimaryLocation W20874781181 @default.
• W2087478118 hasRelatedWork W2018368842 @default.
• W2087478118 hasRelatedWork W2021560856 @default.
• W2087478118 hasRelatedWork W2032639135 @default.
• W2087478118 hasRelatedWork W2034583712 @default.
• W2087478118 hasRelatedWork W2034831606 @default.
• W2087478118 hasRelatedWork W2044826694 @default.
• W2087478118 hasRelatedWork W2063619316 @default.
• W2087478118 hasRelatedWork W2087478118 @default.
• W2087478118 hasRelatedWork W2142714117 @default.
• W2087478118 hasRelatedWork W2462535943 @default.
• W2087478118 hasVolume "56" @default.
• W2087478118 isParatext "false" @default.
• W2087478118 isRetracted "false" @default.
• W2087478118 magId "2087478118" @default.
• W2087478118 workType "article" @default.