Matches in SemOpenAlex for { <https://semopenalex.org/work/W2087484404> ?p ?o ?g. }
Showing items 1 to 89 of
89
with 100 items per page.
- W2087484404 endingPage "46" @default.
- W2087484404 startingPage "44" @default.
- W2087484404 abstract "Efficient detection of HCV RNA in serum is critical for the diagnosis of hepatitis C virus (HCV) infection. Various nucleic acid extraction methods have been used to extract viral RNA for real-time reverse transcription PCR (RT-PCR). However, the efficiencies of extraction methods for HCV RNA in sera collected from individuals with hyperlipidemia, hyperbilirubinemia and hyperglobulinemia have not been investigated. In the present study, the efficiencies of three extraction methods, i.e., Trizol, guanidine isothiocyanate and silica nanoparticles, were evaluated and compared. All serum samples were collected from HCV-infected patients. For serum samples in which bilirubin, lipids and globulins were all within the normal range, the medians of HCV RNA concentration with Trizol, guanidine isothiocyanate and silica method were 2.25 × 104, 2.80 × 104 and 3.26 × 105 IU/ml HCV RNA respectively (n = 180). For hyperlipidemia serum samples, the medians were 6.70 × 103, 8.79 × 103 and 1.10 × 106 respectively (n = 158). For hyperbilirubinemia serum samples, the medians were 5.71 × 104, 1.59 × 105 and 1.09 × 106 respectively (n = 107). For hyperglobulinemia serum samples, the medians were 3.44 × 104, 3.10 × 104 and 3.06 × 105 respectively (n = 71). The medians were highest with silica method from all these types of serum samples. The silica method is, therefore, efficient for HCV RNA extraction even for sera from hyperlipidemia, hyperbilirubinemia and hyperglobulinemia patients." @default.
- W2087484404 created "2016-06-24" @default.
- W2087484404 creator A5064555523 @default.
- W2087484404 creator A5078988597 @default.
- W2087484404 creator A5085537430 @default.
- W2087484404 date "2015-02-01" @default.
- W2087484404 modified "2023-10-16" @default.
- W2087484404 title "Comparison of three methods for extraction of HCV RNA in sera collected from individuals with hyperlipidemia, hyperbilirubinemia and hyperglobulinemia" @default.
- W2087484404 cites W1977028768 @default.
- W2087484404 cites W1977300462 @default.
- W2087484404 cites W1985178609 @default.
- W2087484404 cites W1988419363 @default.
- W2087484404 cites W200626544 @default.
- W2087484404 cites W2006309458 @default.
- W2087484404 cites W2066124462 @default.
- W2087484404 cites W2070652335 @default.
- W2087484404 cites W2094372889 @default.
- W2087484404 cites W2094650655 @default.
- W2087484404 cites W2107512551 @default.
- W2087484404 cites W2135745383 @default.
- W2087484404 cites W2161377485 @default.
- W2087484404 cites W2325791202 @default.
- W2087484404 doi "https://doi.org/10.1016/j.jviromet.2014.11.004" @default.
- W2087484404 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/25445797" @default.
- W2087484404 hasPublicationYear "2015" @default.
- W2087484404 type Work @default.
- W2087484404 sameAs 2087484404 @default.
- W2087484404 citedByCount "4" @default.
- W2087484404 countsByYear W20874844042015 @default.
- W2087484404 countsByYear W20874844042016 @default.
- W2087484404 countsByYear W20874844042017 @default.
- W2087484404 countsByYear W20874844042018 @default.
- W2087484404 crossrefType "journal-article" @default.
- W2087484404 hasAuthorship W2087484404A5064555523 @default.
- W2087484404 hasAuthorship W2087484404A5078988597 @default.
- W2087484404 hasAuthorship W2087484404A5085537430 @default.
- W2087484404 hasConcept C104317684 @default.
- W2087484404 hasConcept C112190416 @default.
- W2087484404 hasConcept C126322002 @default.
- W2087484404 hasConcept C134018914 @default.
- W2087484404 hasConcept C159047783 @default.
- W2087484404 hasConcept C205713591 @default.
- W2087484404 hasConcept C2522874641 @default.
- W2087484404 hasConcept C2776572452 @default.
- W2087484404 hasConcept C2779091943 @default.
- W2087484404 hasConcept C2780593183 @default.
- W2087484404 hasConcept C55493867 @default.
- W2087484404 hasConcept C555293320 @default.
- W2087484404 hasConcept C67705224 @default.
- W2087484404 hasConcept C71924100 @default.
- W2087484404 hasConcept C86803240 @default.
- W2087484404 hasConcept C90924648 @default.
- W2087484404 hasConceptScore W2087484404C104317684 @default.
- W2087484404 hasConceptScore W2087484404C112190416 @default.
- W2087484404 hasConceptScore W2087484404C126322002 @default.
- W2087484404 hasConceptScore W2087484404C134018914 @default.
- W2087484404 hasConceptScore W2087484404C159047783 @default.
- W2087484404 hasConceptScore W2087484404C205713591 @default.
- W2087484404 hasConceptScore W2087484404C2522874641 @default.
- W2087484404 hasConceptScore W2087484404C2776572452 @default.
- W2087484404 hasConceptScore W2087484404C2779091943 @default.
- W2087484404 hasConceptScore W2087484404C2780593183 @default.
- W2087484404 hasConceptScore W2087484404C55493867 @default.
- W2087484404 hasConceptScore W2087484404C555293320 @default.
- W2087484404 hasConceptScore W2087484404C67705224 @default.
- W2087484404 hasConceptScore W2087484404C71924100 @default.
- W2087484404 hasConceptScore W2087484404C86803240 @default.
- W2087484404 hasConceptScore W2087484404C90924648 @default.
- W2087484404 hasLocation W20874844041 @default.
- W2087484404 hasLocation W20874844042 @default.
- W2087484404 hasOpenAccess W2087484404 @default.
- W2087484404 hasPrimaryLocation W20874844041 @default.
- W2087484404 hasRelatedWork W1982203362 @default.
- W2087484404 hasRelatedWork W2348628032 @default.
- W2087484404 hasRelatedWork W2362892540 @default.
- W2087484404 hasRelatedWork W2364543258 @default.
- W2087484404 hasRelatedWork W2365126566 @default.
- W2087484404 hasRelatedWork W2367351498 @default.
- W2087484404 hasRelatedWork W2806739216 @default.
- W2087484404 hasRelatedWork W3102962722 @default.
- W2087484404 hasRelatedWork W4226088443 @default.
- W2087484404 hasRelatedWork W4280539041 @default.
- W2087484404 hasVolume "212" @default.
- W2087484404 isParatext "false" @default.
- W2087484404 isRetracted "false" @default.
- W2087484404 magId "2087484404" @default.
- W2087484404 workType "article" @default.