FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2087496720> ?p ?o ?g. }

• W2087496720 endingPage "963" @default.
• W2087496720 startingPage "958" @default.
• W2087496720 abstract "Eukaryotic subcellular membrane systems, such as the nuclear envelope or endoplasmic reticulum, present a rich array of architecturally and compositionally complex supramolecular targets that are as yet inaccessible. Here we describe layer-by-layer phospholipid membrane assembly on microfluidic droplets, a route to structures with defined compositional asymmetry and lamellarity. Starting with phospholipid-stabilized water-in-oil droplets trapped in a static droplet array, lipid monolayer deposition proceeds as oil/water-phase boundaries pass over the droplets. Unilamellar vesicles assembled layer-by-layer support functional insertion both of purified and of in situ expressed membrane proteins. Synthesis and chemical probing of asymmetric unilamellar and double-bilayer vesicles demonstrate the programmability of both membrane lamellarity and lipid-leaflet composition during assembly. The immobilized vesicle arrays are a pragmatic experimental platform for biophysical studies of membranes and their associated proteins, particularly complexes that assemble and function in multilamellar contexts in vivo. Immobilized microfluidic water-in-oil droplets serve as templates for layer-by-layer deposition of lipid monolayers to create vesicles of programmable lamellarity and content. Arrays of vesicles allow reproducible assembly and multi-vesicle probing of complex membrane-associated parameters, such as permeability, asymmetry and membrane protein function." @default.
• W2087496720 created "2016-06-24" @default.
• W2087496720 creator A5018062150 @default.
• W2087496720 creator A5068171984 @default.
• W2087496720 date "2013-09-29" @default.
• W2087496720 modified "2023-09-28" @default.
• W2087496720 title "Layer-by-layer cell membrane assembly" @default.
• W2087496720 cites W1587481053 @default.
• W2087496720 cites W1967139808 @default.
• W2087496720 cites W1967179899 @default.
• W2087496720 cites W1968562596 @default.
• W2087496720 cites W1968823199 @default.
• W2087496720 cites W1971216177 @default.
• W2087496720 cites W1973513361 @default.
• W2087496720 cites W1976493992 @default.
• W2087496720 cites W1988097700 @default.
• W2087496720 cites W1998664418 @default.
• W2087496720 cites W1999242918 @default.
• W2087496720 cites W2001805380 @default.
• W2087496720 cites W2009434334 @default.
• W2087496720 cites W2009858479 @default.
• W2087496720 cites W2027794151 @default.
• W2087496720 cites W2030683115 @default.
• W2087496720 cites W2038891236 @default.
• W2087496720 cites W2058460324 @default.
• W2087496720 cites W2067510697 @default.
• W2087496720 cites W2074588113 @default.
• W2087496720 cites W2108586733 @default.
• W2087496720 cites W2125872855 @default.
• W2087496720 cites W2132606232 @default.
• W2087496720 cites W2136319664 @default.
• W2087496720 cites W2164386909 @default.
• W2087496720 cites W2166458266 @default.
• W2087496720 cites W2169171478 @default.
• W2087496720 cites W2315802634 @default.
• W2087496720 cites W2321077317 @default.
• W2087496720 cites W2490721346 @default.
• W2087496720 doi "https://doi.org/10.1038/nchem.1765" @default.
• W2087496720 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4003896" @default.
• W2087496720 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/24153375" @default.
• W2087496720 hasPublicationYear "2013" @default.
• W2087496720 type Work @default.
• W2087496720 sameAs 2087496720 @default.
• W2087496720 citedByCount "125" @default.
• W2087496720 countsByYear W20874967202014 @default.
• W2087496720 countsByYear W20874967202015 @default.
• W2087496720 countsByYear W20874967202016 @default.
• W2087496720 countsByYear W20874967202017 @default.
• W2087496720 countsByYear W20874967202018 @default.
• W2087496720 countsByYear W20874967202019 @default.
• W2087496720 countsByYear W20874967202020 @default.
• W2087496720 countsByYear W20874967202021 @default.
• W2087496720 countsByYear W20874967202022 @default.
• W2087496720 countsByYear W20874967202023 @default.
• W2087496720 crossrefType "journal-article" @default.
• W2087496720 hasAuthorship W2087496720A5018062150 @default.
• W2087496720 hasAuthorship W2087496720A5068171984 @default.
• W2087496720 hasBestOaLocation W20874967202 @default.
• W2087496720 hasConcept C12554922 @default.
• W2087496720 hasConcept C130316041 @default.
• W2087496720 hasConcept C158617107 @default.
• W2087496720 hasConcept C171250308 @default.
• W2087496720 hasConcept C185592680 @default.
• W2087496720 hasConcept C192157962 @default.
• W2087496720 hasConcept C192562407 @default.
• W2087496720 hasConcept C2778918659 @default.
• W2087496720 hasConcept C39944091 @default.
• W2087496720 hasConcept C41625074 @default.
• W2087496720 hasConcept C55493867 @default.
• W2087496720 hasConcept C7070889 @default.
• W2087496720 hasConcept C8673954 @default.
• W2087496720 hasConcept C86803240 @default.
• W2087496720 hasConceptScore W2087496720C12554922 @default.
• W2087496720 hasConceptScore W2087496720C130316041 @default.
• W2087496720 hasConceptScore W2087496720C158617107 @default.
• W2087496720 hasConceptScore W2087496720C171250308 @default.
• W2087496720 hasConceptScore W2087496720C185592680 @default.
• W2087496720 hasConceptScore W2087496720C192157962 @default.
• W2087496720 hasConceptScore W2087496720C192562407 @default.
• W2087496720 hasConceptScore W2087496720C2778918659 @default.
• W2087496720 hasConceptScore W2087496720C39944091 @default.
• W2087496720 hasConceptScore W2087496720C41625074 @default.
• W2087496720 hasConceptScore W2087496720C55493867 @default.
• W2087496720 hasConceptScore W2087496720C7070889 @default.
• W2087496720 hasConceptScore W2087496720C8673954 @default.
• W2087496720 hasConceptScore W2087496720C86803240 @default.
• W2087496720 hasIssue "11" @default.
• W2087496720 hasLocation W20874967201 @default.
• W2087496720 hasLocation W20874967202 @default.
• W2087496720 hasLocation W20874967203 @default.
• W2087496720 hasLocation W20874967204 @default.
• W2087496720 hasOpenAccess W2087496720 @default.
• W2087496720 hasPrimaryLocation W20874967201 @default.
• W2087496720 hasRelatedWork W1993582666 @default.
• W2087496720 hasRelatedWork W1995883108 @default.
• W2087496720 hasRelatedWork W2054087967 @default.
• W2087496720 hasRelatedWork W2065191657 @default.
• W2087496720 hasRelatedWork W2080645020 @default.

• next