Matches in SemOpenAlex for { <https://semopenalex.org/work/W2087518534> ?p ?o ?g. }
Showing items 1 to 75 of
75
with 100 items per page.
- W2087518534 abstract "Subdomain 2 of actin, which contains the DNase I binding loop (D-loop, residues 38-52), slightly changes its conformation during actin polymerization and interacts with the C-terminus of the adjacent subunit in actin filament. This region is suggested to be important for actin-myosin interaction: it was found that binding of myosin II induces conformational changes in subdomain 2 and the proteolytic digestion of the D-loop disturbs the motor function of myosin II. However, although as many as 24 classes of myosin have already been found and their in vivo roles are completely different, the contribution of the D-loop to actin-myosin interaction has so far been studied only for myosin II.In this study, to determine whether the D-loop contributes to the interaction with myosin V and if so, in what way it affects its motor function, we prepared actins modified in the D-loop and analyzed the effects of modifications on the motile properties of myosins II and V. We found that the D-loop modifications, namely, the proteolytic digestion with subtilisin and the M47A point mutation, significantly decreased the gliding velocity on myosin II-HMM in an in vitro motility assay, due to a weaker generated force. On the other hand, single molecules of myosin V walked with the same velocity on both the wild-type and modified actins; however, the run lengths decreased sharply, correlating with a lower affinity of myosin for actin due to the D-loop modifications.These results show that the D-loop strongly modulates the force generation by myosin II and the processivity of myosin V, presumably affecting actin-myosin interaction in the A.M.ADP.Pi state of both myosins. Our findings are important to understand the principles how an actin molecule may regulate diverse in vivo functions of various myosin isoforms." @default.
- W2087518534 created "2016-06-24" @default.
- W2087518534 creator A5002112059 @default.
- W2087518534 creator A5067804366 @default.
- W2087518534 creator A5089339287 @default.
- W2087518534 date "2010-01-01" @default.
- W2087518534 modified "2023-09-29" @default.
- W2087518534 title "Regulation of Myosin Motility by D-Loop of Actin" @default.
- W2087518534 doi "https://doi.org/10.1016/j.bpj.2009.12.3037" @default.
- W2087518534 hasPublicationYear "2010" @default.
- W2087518534 type Work @default.
- W2087518534 sameAs 2087518534 @default.
- W2087518534 citedByCount "0" @default.
- W2087518534 crossrefType "journal-article" @default.
- W2087518534 hasAuthorship W2087518534A5002112059 @default.
- W2087518534 hasAuthorship W2087518534A5067804366 @default.
- W2087518534 hasAuthorship W2087518534A5089339287 @default.
- W2087518534 hasBestOaLocation W20875185341 @default.
- W2087518534 hasConcept C114614502 @default.
- W2087518534 hasConcept C12554922 @default.
- W2087518534 hasConcept C125705527 @default.
- W2087518534 hasConcept C15954220 @default.
- W2087518534 hasConcept C160408235 @default.
- W2087518534 hasConcept C184670325 @default.
- W2087518534 hasConcept C185592680 @default.
- W2087518534 hasConcept C18923293 @default.
- W2087518534 hasConcept C33923547 @default.
- W2087518534 hasConcept C55493867 @default.
- W2087518534 hasConcept C58207958 @default.
- W2087518534 hasConcept C6997183 @default.
- W2087518534 hasConcept C8035138 @default.
- W2087518534 hasConcept C86803240 @default.
- W2087518534 hasConcept C95444343 @default.
- W2087518534 hasConceptScore W2087518534C114614502 @default.
- W2087518534 hasConceptScore W2087518534C12554922 @default.
- W2087518534 hasConceptScore W2087518534C125705527 @default.
- W2087518534 hasConceptScore W2087518534C15954220 @default.
- W2087518534 hasConceptScore W2087518534C160408235 @default.
- W2087518534 hasConceptScore W2087518534C184670325 @default.
- W2087518534 hasConceptScore W2087518534C185592680 @default.
- W2087518534 hasConceptScore W2087518534C18923293 @default.
- W2087518534 hasConceptScore W2087518534C33923547 @default.
- W2087518534 hasConceptScore W2087518534C55493867 @default.
- W2087518534 hasConceptScore W2087518534C58207958 @default.
- W2087518534 hasConceptScore W2087518534C6997183 @default.
- W2087518534 hasConceptScore W2087518534C8035138 @default.
- W2087518534 hasConceptScore W2087518534C86803240 @default.
- W2087518534 hasConceptScore W2087518534C95444343 @default.
- W2087518534 hasLocation W20875185341 @default.
- W2087518534 hasOpenAccess W2087518534 @default.
- W2087518534 hasPrimaryLocation W20875185341 @default.
- W2087518534 hasRelatedWork W1753223793 @default.
- W2087518534 hasRelatedWork W1979496410 @default.
- W2087518534 hasRelatedWork W1989722298 @default.
- W2087518534 hasRelatedWork W1995883317 @default.
- W2087518534 hasRelatedWork W1998841338 @default.
- W2087518534 hasRelatedWork W2015431303 @default.
- W2087518534 hasRelatedWork W2045431616 @default.
- W2087518534 hasRelatedWork W2053466049 @default.
- W2087518534 hasRelatedWork W2085239860 @default.
- W2087518534 hasRelatedWork W2092723260 @default.
- W2087518534 hasRelatedWork W2116675254 @default.
- W2087518534 hasRelatedWork W2130398467 @default.
- W2087518534 hasRelatedWork W2165805421 @default.
- W2087518534 hasRelatedWork W2165817597 @default.
- W2087518534 hasRelatedWork W2260600391 @default.
- W2087518534 hasRelatedWork W269020593 @default.
- W2087518534 hasRelatedWork W2791659349 @default.
- W2087518534 hasRelatedWork W2966272313 @default.
- W2087518534 hasRelatedWork W3021924308 @default.
- W2087518534 hasRelatedWork W58002258 @default.
- W2087518534 isParatext "false" @default.
- W2087518534 isRetracted "false" @default.
- W2087518534 magId "2087518534" @default.
- W2087518534 workType "article" @default.