FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2087573072> ?p ?o ?g. }

• W2087573072 endingPage "13792" @default.
• W2087573072 startingPage "13787" @default.
• W2087573072 abstract "Antisense oligodeoxynucleotides (AONs) and short interfering RNAs (siRNAs) effect posttranscriptional gene silencing (PTGS) by hybridizing to an mRNA and then directing its cleavage. To understand the constraints that mRNA structure imposes on AON- vs. siRNA-mediated PTGS, AON- and siRNA-mediated cleavage of defined mRNA structures was monitored in Drosophila embryo whole-cell lysates. We observed that AON-directed cleavage was ≈3-fold faster than cleavage with a siRNA directed to the same target site. Furthermore, and unexpectedly, AON-mediated cleavage was found to be much less fastidious with respect to target sequence accessibility, as measured by the presence of unpaired nucleotides, than a corresponding siRNA. Nonetheless, in vivo , siRNAs silenced their mRNA target at least 2-fold more efficiently than the corresponding AON. These seemingly contradictory results suggested that additional, as yet undefined factors play an important role in regulating PTGS efficiency in vivo . We used a well defined RNA-binding protein, αCP, and its corresponding high-affinity RNA-binding site to explore this hypothesis. We found that prebound αCP effectively blocked AON-mediated cleavage of the RNA-binding site compared with cleavage of the site in the absence of αCP. We conclude that higher-order structures formed by RNA and bound proteins play an important role in determining the efficiency of AON-directed PTGS. We hypothesize that strategies aimed at removing RNA-binding proteins might significantly improve AON-mediated PTGS in vivo ." @default.
• W2087573072 created "2016-06-24" @default.
• W2087573072 creator A5019594438 @default.
• W2087573072 creator A5026226715 @default.
• W2087573072 creator A5061278758 @default.
• W2087573072 creator A5077260667 @default.
• W2087573072 creator A5080563290 @default.
• W2087573072 date "2008-09-16" @default.
• W2087573072 modified "2023-10-03" @default.
• W2087573072 title "Effects of local mRNA structure on posttranscriptional gene silencing" @default.
• W2087573072 cites W1501059295 @default.
• W2087573072 cites W1528641594 @default.
• W2087573072 cites W1564730852 @default.
• W2087573072 cites W1611419331 @default.
• W2087573072 cites W1964227936 @default.
• W2087573072 cites W1964997792 @default.
• W2087573072 cites W1970679679 @default.
• W2087573072 cites W1979691620 @default.
• W2087573072 cites W1982944995 @default.
• W2087573072 cites W1984006325 @default.
• W2087573072 cites W1984912016 @default.
• W2087573072 cites W1985584330 @default.
• W2087573072 cites W1994983564 @default.
• W2087573072 cites W2011879414 @default.
• W2087573072 cites W2012538868 @default.
• W2087573072 cites W2019387053 @default.
• W2087573072 cites W2026978183 @default.
• W2087573072 cites W2042973755 @default.
• W2087573072 cites W2054757478 @default.
• W2087573072 cites W2062290881 @default.
• W2087573072 cites W2067747544 @default.
• W2087573072 cites W2071560471 @default.
• W2087573072 cites W2074106269 @default.
• W2087573072 cites W2079707100 @default.
• W2087573072 cites W2082213106 @default.
• W2087573072 cites W2087672655 @default.
• W2087573072 cites W2088137097 @default.
• W2087573072 cites W2088279886 @default.
• W2087573072 cites W2095509700 @default.
• W2087573072 cites W2096095309 @default.
• W2087573072 cites W2101296752 @default.
• W2087573072 cites W2115018128 @default.
• W2087573072 cites W2127609664 @default.
• W2087573072 cites W2138668121 @default.
• W2087573072 cites W2145638174 @default.
• W2087573072 cites W2158605421 @default.
• W2087573072 cites W2166802510 @default.
• W2087573072 cites W2166915947 @default.
• W2087573072 doi "https://doi.org/10.1073/pnas.0805781105" @default.
• W2087573072 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2544532" @default.
• W2087573072 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/18784366" @default.
• W2087573072 hasPublicationYear "2008" @default.
• W2087573072 type Work @default.
• W2087573072 sameAs 2087573072 @default.
• W2087573072 citedByCount "27" @default.
• W2087573072 countsByYear W20875730722014 @default.
• W2087573072 countsByYear W20875730722015 @default.
• W2087573072 countsByYear W20875730722018 @default.
• W2087573072 countsByYear W20875730722019 @default.
• W2087573072 countsByYear W20875730722020 @default.
• W2087573072 countsByYear W20875730722021 @default.
• W2087573072 countsByYear W20875730722023 @default.
• W2087573072 crossrefType "journal-article" @default.
• W2087573072 hasAuthorship W2087573072A5019594438 @default.
• W2087573072 hasAuthorship W2087573072A5026226715 @default.
• W2087573072 hasAuthorship W2087573072A5061278758 @default.
• W2087573072 hasAuthorship W2087573072A5077260667 @default.
• W2087573072 hasAuthorship W2087573072A5080563290 @default.
• W2087573072 hasBestOaLocation W20875730721 @default.
• W2087573072 hasConcept C104317684 @default.
• W2087573072 hasConcept C105580179 @default.
• W2087573072 hasConcept C119056186 @default.
• W2087573072 hasConcept C150194340 @default.
• W2087573072 hasConcept C151730666 @default.
• W2087573072 hasConcept C153911025 @default.
• W2087573072 hasConcept C166703698 @default.
• W2087573072 hasConcept C175156509 @default.
• W2087573072 hasConcept C22615655 @default.
• W2087573072 hasConcept C43369102 @default.
• W2087573072 hasConcept C54355233 @default.
• W2087573072 hasConcept C60723119 @default.
• W2087573072 hasConcept C67705224 @default.
• W2087573072 hasConcept C86803240 @default.
• W2087573072 hasConcept C95444343 @default.
• W2087573072 hasConceptScore W2087573072C104317684 @default.
• W2087573072 hasConceptScore W2087573072C105580179 @default.
• W2087573072 hasConceptScore W2087573072C119056186 @default.
• W2087573072 hasConceptScore W2087573072C150194340 @default.
• W2087573072 hasConceptScore W2087573072C151730666 @default.
• W2087573072 hasConceptScore W2087573072C153911025 @default.
• W2087573072 hasConceptScore W2087573072C166703698 @default.
• W2087573072 hasConceptScore W2087573072C175156509 @default.
• W2087573072 hasConceptScore W2087573072C22615655 @default.
• W2087573072 hasConceptScore W2087573072C43369102 @default.
• W2087573072 hasConceptScore W2087573072C54355233 @default.
• W2087573072 hasConceptScore W2087573072C60723119 @default.
• W2087573072 hasConceptScore W2087573072C67705224 @default.
• W2087573072 hasConceptScore W2087573072C86803240 @default.

• next