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• W2087592072 abstract "Investigation of the retina proteome during hypoxia-induced retinal neovascularization is valuable for understanding pathogenesis of retinopathy of prematurity (ROP). Here we employed a reproducible ion-current-based MS1 quantification approach (ICB) to explore the retinal proteomic changes in early stage of ROP in a rat model of oxygen-induced retinopathy (OIR). Retina proteins, which are rich in membrane proteins, were efficiently extracted by a detergent-cocktail and subjected to precipitation/on-pellet-digestion, followed by nano-LC-MS analysis on a 75-cm column with a 7-h gradient. The high reproducibility of sample preparation and chromatography separation enabled excellent peak alignment and contributed to the superior performance of ICB over parallel label-free approaches. In this study, sum-of-intensity with rejection was incorporated to determine the protein ratios. In total, 1325 unique protein groups were quantified from rat retinas (n = 4/group) with at least two distinct peptides at a protein FDR of 1%. Thirty-two significantly altered proteins were observed with confidence, and the elevated glial fibrillary acidic protein and decreased crystalline proteins in OIR retinas agree well with previous studies. Selected key alterations were further validated by Western blot analysis. Interestingly, Rab21/RhoA/ROCK2/moesin signaling pathway was found to be involved in retinal neovascularization of OIR. Moreover, highly elevated annexin A3, a potential angiogenic mediator, was observed in OIR retinas and may serve as a potential therapeutic target. In conclusion, reproducible ICB profiling enabled reliable discovery of many altered mediators and pathways in OIR retinas, thereby providing new insights into molecular mechanisms involved in pathogenesis of ROP." @default.
• W2087592072 created "2016-06-24" @default.
• W2087592072 creator A5027835055 @default.
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• W2087592072 date "2015-04-06" @default.
• W2087592072 modified "2023-09-26" @default.
• W2087592072 title "Proteomic Profiling of the Retinas in a Neonatal Rat Model of Oxygen-Induced Retinopathy with a Reproducible Ion-Current-Based MS1 Approach" @default.
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• W2087592072 cites W1517815925 @default.
• W2087592072 cites W1538850399 @default.
• W2087592072 cites W1562028693 @default.
• W2087592072 cites W1656028612 @default.
• W2087592072 cites W1731074416 @default.
• W2087592072 cites W178239812 @default.
• W2087592072 cites W1827885532 @default.
• W2087592072 cites W189579241 @default.
• W2087592072 cites W1965087659 @default.
• W2087592072 cites W1965663571 @default.
• W2087592072 cites W1966189629 @default.
• W2087592072 cites W1969488098 @default.
• W2087592072 cites W1970971784 @default.
• W2087592072 cites W1974247935 @default.
• W2087592072 cites W1978542424 @default.
• W2087592072 cites W1990521929 @default.
• W2087592072 cites W1995881304 @default.
• W2087592072 cites W1997047460 @default.
• W2087592072 cites W2003999217 @default.
• W2087592072 cites W2004062688 @default.
• W2087592072 cites W2009113844 @default.
• W2087592072 cites W2009567773 @default.
• W2087592072 cites W2011061591 @default.
• W2087592072 cites W2012073192 @default.
• W2087592072 cites W2012301205 @default.
• W2087592072 cites W2017303840 @default.
• W2087592072 cites W2020466321 @default.
• W2087592072 cites W2027765716 @default.
• W2087592072 cites W2029557435 @default.
• W2087592072 cites W2032110888 @default.
• W2087592072 cites W2036010017 @default.
• W2087592072 cites W2037517553 @default.
• W2087592072 cites W2038249420 @default.
• W2087592072 cites W2039380477 @default.
• W2087592072 cites W2041669106 @default.
• W2087592072 cites W2045032064 @default.
• W2087592072 cites W2045739383 @default.
• W2087592072 cites W2048520987 @default.
• W2087592072 cites W2052154747 @default.
• W2087592072 cites W2053098204 @default.
• W2087592072 cites W2056972503 @default.
• W2087592072 cites W2058512024 @default.
• W2087592072 cites W2058545300 @default.
• W2087592072 cites W2065046804 @default.
• W2087592072 cites W2065296970 @default.
• W2087592072 cites W2078138443 @default.
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• W2087592072 cites W2106947018 @default.
• W2087592072 cites W2112451265 @default.
• W2087592072 cites W2117327695 @default.
• W2087592072 cites W2123886946 @default.
• W2087592072 cites W2131019246 @default.
• W2087592072 cites W2136359150 @default.
• W2087592072 cites W2136595794 @default.
• W2087592072 cites W2139769620 @default.
• W2087592072 cites W2141816935 @default.
• W2087592072 cites W2148490163 @default.
• W2087592072 cites W2153562609 @default.
• W2087592072 cites W2156382689 @default.
• W2087592072 cites W2158217645 @default.
• W2087592072 cites W2161902068 @default.
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• W2087592072 cites W2319494906 @default.
• W2087592072 cites W2324539199 @default.
• W2087592072 cites W2329436915 @default.
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• W2087592072 doi "https://doi.org/10.1021/pr501238m" @default.
• W2087592072 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4456185" @default.
• W2087592072 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/25780855" @default.
• W2087592072 hasPublicationYear "2015" @default.
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