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- W2087642779 abstract "Chimeric proteins were constructed to define the nuclear localization signals (NLSs) of human ribosomal protein S6. The complete cDNA sequence, different cDNA fragments and oligonucleotides of the human ribosomal proteins S6, respectively, were joined to the 5' end of the entire LacZ gene of Escherichia coli by using recombinant techniques. The hybrid genes were transfected into L cells, transiently expressed, and the intracellular location of the fusion proteins was determined by their beta-galactosidase activity. Three NLSs were identified in the C-terminal half of the S6 protein. Deletion mutagenesis demonstrated that a single NLS is sufficient for targeting the corresponding S6-beta-galactosidase chimera into the nucleus. Removal of all three putative NLSs completely blocked the nuclear import of the resulting S6-beta-galactosidase fusion protein, which instead became evenly distributed in the cytoplasm. Chimeras containing deletion mutants of S6 with at least one single NLS or unmodified S6 accumulated in the nucleolus. Analysis of several constructs reveals the existence of a specific domain that is essential but not sufficient for nucleolar accumulation of S6." @default.
- W2087642779 created "2016-06-24" @default.
- W2087642779 creator A5029286763 @default.
- W2087642779 creator A5034449909 @default.
- W2087642779 creator A5076187973 @default.
- W2087642779 date "1995-12-01" @default.
- W2087642779 modified "2023-09-24" @default.
- W2087642779 title "Nuclear and nucleolar targeting of human ribosomal protein S6." @default.
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- W2087642779 cites W1516389463 @default.
- W2087642779 cites W1526839960 @default.
- W2087642779 cites W1533075742 @default.
- W2087642779 cites W1540559679 @default.
- W2087642779 cites W1576538493 @default.
- W2087642779 cites W1587564178 @default.
- W2087642779 cites W1590665808 @default.
- W2087642779 cites W1595534151 @default.
- W2087642779 cites W1679428278 @default.
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- W2087642779 cites W2165635695 @default.
- W2087642779 cites W2178029086 @default.
- W2087642779 cites W2428494219 @default.
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- W2087642779 doi "https://doi.org/10.1091/mbc.6.12.1875" @default.
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