Matches in SemOpenAlex for { <https://semopenalex.org/work/W2087645897> ?p ?o ?g. }
Showing items 1 to 98 of
98
with 100 items per page.
- W2087645897 endingPage "1315" @default.
- W2087645897 startingPage "1308" @default.
- W2087645897 abstract "Specific primers can be used in polymerase chain reactions (PCR) to amplify prey DNA from the gut content of generalist predators at high specificity and sensitivity. A prerequisite for applying this approach to field studies, however, is to confirm that primers are actually targeting specific prey species or prey groups and do not produce false positive results by amplifying DNA either from predator species or from the wide range of potential alternative prey found under natural conditions. Here, we report on a new group-specific primer pair for earthworms designed from cytochrome oxidase c subunit 1 (COI) sequences of 11 earthworm species found in Central Europe that can be used to detect consumption of earthworms by invertebrate predators. Besides inter-specific also considerable intra-specific variation was found for COI sequences among most of the earthworm species. We, therefore, combined a universal forward primer with an earthworm-specific reverse primer which amplified a 523 bp product from all 11 species tested. Earthworm DNA amplification was also successful in the presence of excess DNA of a predator species. The primer pair was tested against 82 non-target invertebrate species commonly found in the same habitats, including potential prey for generalist predators and predators themselves. The earthworm primer was highly specific: only one of the non-target species showed a product of similar length as the earthworms, whereas PCR with 12 non-target species produced amplicons whose length differed from that of earthworms. We conclude that the new primer will be a useful tool to investigate the role earthworms play as a food resource in soil food-webs. Moreover, we suggest that future studies utilizing DNA-based approaches for prey detection should select non-target species for cross-reactivity tests according to their abundance and importance rather than choosing representatives of taxonomic units; this will help validate the results achieved using species- or group-specific primers and guarantee their meaningful ecological interpretation." @default.
- W2087645897 created "2016-06-24" @default.
- W2087645897 creator A5010246228 @default.
- W2087645897 creator A5048592793 @default.
- W2087645897 creator A5072711258 @default.
- W2087645897 date "2006-06-01" @default.
- W2087645897 modified "2023-10-18" @default.
- W2087645897 title "Earthworm primers for DNA-based gut content analysis and their cross-reactivity in a multi-species system" @default.
- W2087645897 cites W1895353642 @default.
- W2087645897 cites W1948727601 @default.
- W2087645897 cites W1978910316 @default.
- W2087645897 cites W1980638571 @default.
- W2087645897 cites W1980678363 @default.
- W2087645897 cites W1997794383 @default.
- W2087645897 cites W2005447944 @default.
- W2087645897 cites W2037630741 @default.
- W2087645897 cites W2045333059 @default.
- W2087645897 cites W2053537794 @default.
- W2087645897 cites W2055266893 @default.
- W2087645897 cites W2055978059 @default.
- W2087645897 cites W2083453790 @default.
- W2087645897 cites W2102253750 @default.
- W2087645897 cites W2111609575 @default.
- W2087645897 cites W2114703217 @default.
- W2087645897 cites W2122399862 @default.
- W2087645897 cites W2132823440 @default.
- W2087645897 cites W2141718064 @default.
- W2087645897 cites W2154472735 @default.
- W2087645897 cites W2157912267 @default.
- W2087645897 cites W2158175796 @default.
- W2087645897 cites W2170774199 @default.
- W2087645897 cites W2537458484 @default.
- W2087645897 cites W2605342139 @default.
- W2087645897 cites W4236999468 @default.
- W2087645897 doi "https://doi.org/10.1016/j.soilbio.2005.08.019" @default.
- W2087645897 hasPublicationYear "2006" @default.
- W2087645897 type Work @default.
- W2087645897 sameAs 2087645897 @default.
- W2087645897 citedByCount "45" @default.
- W2087645897 countsByYear W20876458972012 @default.
- W2087645897 countsByYear W20876458972013 @default.
- W2087645897 countsByYear W20876458972014 @default.
- W2087645897 countsByYear W20876458972016 @default.
- W2087645897 countsByYear W20876458972017 @default.
- W2087645897 countsByYear W20876458972019 @default.
- W2087645897 countsByYear W20876458972022 @default.
- W2087645897 countsByYear W20876458972023 @default.
- W2087645897 crossrefType "journal-article" @default.
- W2087645897 hasAuthorship W2087645897A5010246228 @default.
- W2087645897 hasAuthorship W2087645897A5048592793 @default.
- W2087645897 hasAuthorship W2087645897A5072711258 @default.
- W2087645897 hasConcept C13474642 @default.
- W2087645897 hasConcept C141732470 @default.
- W2087645897 hasConcept C178790620 @default.
- W2087645897 hasConcept C185592680 @default.
- W2087645897 hasConcept C185933670 @default.
- W2087645897 hasConcept C188382862 @default.
- W2087645897 hasConcept C18903297 @default.
- W2087645897 hasConcept C2776332788 @default.
- W2087645897 hasConcept C2776809823 @default.
- W2087645897 hasConcept C2777563447 @default.
- W2087645897 hasConcept C45371612 @default.
- W2087645897 hasConcept C86803240 @default.
- W2087645897 hasConcept C90856448 @default.
- W2087645897 hasConceptScore W2087645897C13474642 @default.
- W2087645897 hasConceptScore W2087645897C141732470 @default.
- W2087645897 hasConceptScore W2087645897C178790620 @default.
- W2087645897 hasConceptScore W2087645897C185592680 @default.
- W2087645897 hasConceptScore W2087645897C185933670 @default.
- W2087645897 hasConceptScore W2087645897C188382862 @default.
- W2087645897 hasConceptScore W2087645897C18903297 @default.
- W2087645897 hasConceptScore W2087645897C2776332788 @default.
- W2087645897 hasConceptScore W2087645897C2776809823 @default.
- W2087645897 hasConceptScore W2087645897C2777563447 @default.
- W2087645897 hasConceptScore W2087645897C45371612 @default.
- W2087645897 hasConceptScore W2087645897C86803240 @default.
- W2087645897 hasConceptScore W2087645897C90856448 @default.
- W2087645897 hasIssue "6" @default.
- W2087645897 hasLocation W20876458971 @default.
- W2087645897 hasOpenAccess W2087645897 @default.
- W2087645897 hasPrimaryLocation W20876458971 @default.
- W2087645897 hasRelatedWork W152618080 @default.
- W2087645897 hasRelatedWork W2014453109 @default.
- W2087645897 hasRelatedWork W2055266893 @default.
- W2087645897 hasRelatedWork W2099871331 @default.
- W2087645897 hasRelatedWork W2148937907 @default.
- W2087645897 hasRelatedWork W2150436117 @default.
- W2087645897 hasRelatedWork W2157473278 @default.
- W2087645897 hasRelatedWork W2188651226 @default.
- W2087645897 hasRelatedWork W2989764147 @default.
- W2087645897 hasRelatedWork W4244628857 @default.
- W2087645897 hasVolume "38" @default.
- W2087645897 isParatext "false" @default.
- W2087645897 isRetracted "false" @default.
- W2087645897 magId "2087645897" @default.
- W2087645897 workType "article" @default.