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• W2087661650 abstract "ABSTRACT Adipose tissue plays a central role in energy homeostasis. In a previous report, we reported adipogenin, an adipocyte-spe-cific membrane protein whose expression is higher in fat and increases during the adipocyte differentiation of 3T3-L1 cells,as a candidate gene for involvement in adipogenesis. Here, we isolated the bovine adipogenin gene using in silico cloningand the target region for polymerase chain reaction amplification in the database. cDNA, including a 246 base pair (bp)open reading frame, was identified in the adipose tissues of cattle. In silico gene analysis showed that the bovine adipo-genin-encoded protein has a predicted MW of 9882.45, encoding 81 amino acids and located on bovine chromosome 13.The deduced amino acid sequence of bovine adipogenin conserved 86%, 66% and 78% identity with that in pigs, miceand humans, respectively. A semi-quantitative reverse transcriptase polymerase chain reaction showed that bovine adipo-genin mRNA was more highly expressed in subcutaneous, perirenal and mescentric adipose tissue than in non-adipose tis-sue. The level of bovine adipogenin mRNA is dramatically elevated during the adipocyte differentiation of four differentkinds of preadipocytes prepared from subcutaneous, perirenal, mesenteric and parametrial adipose tissues. Our results sug-gest that adipogenin plays an important role in the regulation of adipocyte development in cattle and may be a strong can-didate gene for obesity in mammals." @default.
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• W2087661650 date "2006-12-01" @default.
• W2087661650 modified "2023-09-24" @default.
• W2087661650 title "Molecular cloning and expression analysis of cDNA encoding bovine adipogenin" @default.
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• W2087661650 doi "https://doi.org/10.1111/j.1740-0929.2006.00393.x" @default.
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