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- W2087665562 abstract "A competitive enzyme-linked immunosorbent assay (ELISA) for pentachloronitrobenzene (PCNB), a fungicide and chemical intermediate, was developed using a polyclonal antiserum produced against a hapten−protein conjugate of pentachlorophenoxypropionic acid−bovine serum albumin (BSA). An indirect competitive ELISA of PCNB showed an IC50 of 37 ng/mL and a limit of detection (LOD) of 7 ng/mL. The ELISA can tolerate up to 10% (v/v) methanol, 5% (v/v) acetonitrile, or 5% (v/v) acetone without significant fluctuation of Amax and IC50. The assay sensitivity showed little change in a range of pH from 6 to 8 and concentrations of 0.05−0.2 M NaCl in the assay buffer. Very low cross-reactivities were observed for some structurally related compounds except for hexachlorobenzene (12%). The average recoveries of PCNB from fortified well water, river water, and soil samples were in ranges of 88−94, 80−91, and 70−81%, respectively. The correlations between the gas chromatographic and ELISA results were excellent (r 2 ≥ 0.97, slopes from 0.86 to 1.10) for those fortified samples. The ELISA is a good alternative tool for monitoring PCNB residues in environmental samples. Keywords: Pentachloronitrobenzene; ELISA; fungicide; water; soil" @default.
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- W2087665562 date "2007-04-25" @default.
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- W2087665562 title "Development of an Enzyme-Linked Immunosorbent Assay for the Detection of Pentachloronitrobenzene Residues in Environmental Samples" @default.
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- W2087665562 doi "https://doi.org/10.1021/jf063287d" @default.
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