FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2087675271> ?p ?o ?g. }

• W2087675271 endingPage "35876" @default.
• W2087675271 startingPage "35868" @default.
• W2087675271 abstract "The potato (Solanum tuberosum) disease resistance protein Rx has a modular arrangement that contains coiled-coil (CC), nucleotide-binding (NB), and leucine-rich repeat (LRR) domains and mediates resistance to potato virus X. The Rx N-terminal CC domain undergoes an intramolecular interaction with the Rx NB-LRR region and an intermolecular interaction with the Rx cofactor RanGAP2 (Ran GTPase-activating protein 2). Here, we report the crystal structure of the Rx CC domain in complex with the Trp-Pro-Pro (WPP) domain of RanGAP2. The structure reveals that the Rx CC domain forms a heterodimer with RanGAP2, in striking contrast to the homodimeric structure of the CC domain of the barley disease resistance protein MLA10. Structure-based mutagenesis identified residues from both the Rx CC domain and the RanGAP2 WPP domain that are crucial for their interaction and function in vitro and in vivo. Our results reveal the molecular mechanism underlying the interaction of Rx with RanGAP2 and identify the distinct surfaces of the Rx CC domain that are involved in intramolecular and intermolecular interactions. The potato (Solanum tuberosum) disease resistance protein Rx has a modular arrangement that contains coiled-coil (CC), nucleotide-binding (NB), and leucine-rich repeat (LRR) domains and mediates resistance to potato virus X. The Rx N-terminal CC domain undergoes an intramolecular interaction with the Rx NB-LRR region and an intermolecular interaction with the Rx cofactor RanGAP2 (Ran GTPase-activating protein 2). Here, we report the crystal structure of the Rx CC domain in complex with the Trp-Pro-Pro (WPP) domain of RanGAP2. The structure reveals that the Rx CC domain forms a heterodimer with RanGAP2, in striking contrast to the homodimeric structure of the CC domain of the barley disease resistance protein MLA10. Structure-based mutagenesis identified residues from both the Rx CC domain and the RanGAP2 WPP domain that are crucial for their interaction and function in vitro and in vivo. Our results reveal the molecular mechanism underlying the interaction of Rx with RanGAP2 and identify the distinct surfaces of the Rx CC domain that are involved in intramolecular and intermolecular interactions." @default.
• W2087675271 created "2016-06-24" @default.
• W2087675271 creator A5025232957 @default.
• W2087675271 creator A5029053030 @default.
• W2087675271 creator A5053458834 @default.
• W2087675271 creator A5086027154 @default.
• W2087675271 date "2013-12-01" @default.
• W2087675271 modified "2023-10-13" @default.
• W2087675271 title "Structural Basis for the Interaction between the Potato Virus X Resistance Protein (Rx) and Its Cofactor Ran GTPase-activating Protein 2 (RanGAP2)" @default.
• W2087675271 cites W1977322059 @default.
• W2087675271 cites W1989581558 @default.
• W2087675271 cites W1992014522 @default.
• W2087675271 cites W1996573117 @default.
• W2087675271 cites W2013429621 @default.
• W2087675271 cites W2026130700 @default.
• W2087675271 cites W2029119178 @default.
• W2087675271 cites W2041074318 @default.
• W2087675271 cites W2049579557 @default.
• W2087675271 cites W2074888340 @default.
• W2087675271 cites W2081748829 @default.
• W2087675271 cites W2090723161 @default.
• W2087675271 cites W2090881705 @default.
• W2087675271 cites W2100464143 @default.
• W2087675271 cites W2105850758 @default.
• W2087675271 cites W2123679376 @default.
• W2087675271 cites W2130330131 @default.
• W2087675271 cites W2135353885 @default.
• W2087675271 cites W2137248850 @default.
• W2087675271 cites W2138472234 @default.
• W2087675271 cites W2138659486 @default.
• W2087675271 cites W2139134943 @default.
• W2087675271 cites W2141636493 @default.
• W2087675271 cites W2144081223 @default.
• W2087675271 cites W2145611203 @default.
• W2087675271 cites W2150069840 @default.
• W2087675271 cites W2154203650 @default.
• W2087675271 cites W2154540205 @default.
• W2087675271 cites W2160155425 @default.
• W2087675271 cites W2161496396 @default.
• W2087675271 cites W2162515800 @default.
• W2087675271 cites W2162800927 @default.
• W2087675271 cites W2180229411 @default.
• W2087675271 cites W4232729468 @default.
• W2087675271 doi "https://doi.org/10.1074/jbc.m113.517417" @default.
• W2087675271 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3861636" @default.
• W2087675271 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/24194517" @default.
• W2087675271 hasPublicationYear "2013" @default.
• W2087675271 type Work @default.
• W2087675271 sameAs 2087675271 @default.
• W2087675271 citedByCount "98" @default.
• W2087675271 countsByYear W20876752712014 @default.
• W2087675271 countsByYear W20876752712015 @default.
• W2087675271 countsByYear W20876752712016 @default.
• W2087675271 countsByYear W20876752712017 @default.
• W2087675271 countsByYear W20876752712018 @default.
• W2087675271 countsByYear W20876752712019 @default.
• W2087675271 countsByYear W20876752712020 @default.
• W2087675271 countsByYear W20876752712021 @default.
• W2087675271 countsByYear W20876752712022 @default.
• W2087675271 countsByYear W20876752712023 @default.
• W2087675271 crossrefType "journal-article" @default.
• W2087675271 hasAuthorship W2087675271A5025232957 @default.
• W2087675271 hasAuthorship W2087675271A5029053030 @default.
• W2087675271 hasAuthorship W2087675271A5053458834 @default.
• W2087675271 hasAuthorship W2087675271A5086027154 @default.
• W2087675271 hasBestOaLocation W20876752711 @default.
• W2087675271 hasConcept C104317684 @default.
• W2087675271 hasConcept C113027372 @default.
• W2087675271 hasConcept C144292202 @default.
• W2087675271 hasConcept C160704184 @default.
• W2087675271 hasConcept C170493617 @default.
• W2087675271 hasConcept C185592680 @default.
• W2087675271 hasConcept C207332259 @default.
• W2087675271 hasConcept C47701112 @default.
• W2087675271 hasConcept C512185932 @default.
• W2087675271 hasConcept C55493867 @default.
• W2087675271 hasConcept C55988834 @default.
• W2087675271 hasConcept C71240020 @default.
• W2087675271 hasConcept C86803240 @default.
• W2087675271 hasConcept C99545290 @default.
• W2087675271 hasConceptScore W2087675271C104317684 @default.
• W2087675271 hasConceptScore W2087675271C113027372 @default.
• W2087675271 hasConceptScore W2087675271C144292202 @default.
• W2087675271 hasConceptScore W2087675271C160704184 @default.
• W2087675271 hasConceptScore W2087675271C170493617 @default.
• W2087675271 hasConceptScore W2087675271C185592680 @default.
• W2087675271 hasConceptScore W2087675271C207332259 @default.
• W2087675271 hasConceptScore W2087675271C47701112 @default.
• W2087675271 hasConceptScore W2087675271C512185932 @default.
• W2087675271 hasConceptScore W2087675271C55493867 @default.
• W2087675271 hasConceptScore W2087675271C55988834 @default.
• W2087675271 hasConceptScore W2087675271C71240020 @default.
• W2087675271 hasConceptScore W2087675271C86803240 @default.
• W2087675271 hasConceptScore W2087675271C99545290 @default.
• W2087675271 hasIssue "50" @default.
• W2087675271 hasLocation W20876752711 @default.
• W2087675271 hasLocation W20876752712 @default.
• W2087675271 hasLocation W20876752713 @default.

• next