FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2087675721> ?p ?o ?g. }

• W2087675721 endingPage "31124" @default.
• W2087675721 startingPage "31116" @default.
• W2087675721 abstract "SepF (Septum Forming) protein has been recently identified through genetic studies, and it has been suggested to be involved in the division of Bacillus subtilis cells. We have purified functional B. subtilis SepF from the inclusion bodies overexpressed in Escherichia coli. Far-UV circular dichroism and fluorescence spectroscopic analysis involving the extrinsic fluorescent probe 1-anilinonaphthalene-8-sulfonic acid suggested that the purified SepF had characteristics of folded proteins. SepF was found to promote the assembly and bundling of FtsZ protofilaments using three complimentary techniques, namely 90 degrees light scattering, sedimentation, and transmission electron microscopy. SepF also decreased the critical concentration of FtsZ assembly, prevented the dilution-induced disassembly of FtsZ protofilaments, and suppressed the GTPase activity of FtsZ. Further, thick bundles of FtsZ protofilaments were observed using fluorescein isothiocyanate-labeled SepF (FITC-SepF). Interestingly, FITC-SepF was found to be uniformly distributed along the length of the FtsZ protofilaments, suggesting that SepF copolymerizes with FtsZ. SepF formed a stable complex with FtsZ, as evident from the gel filtration analysis. Using a C-terminal tail truncated FtsZ (FtsZDelta16) and a C-terminal synthetic peptide of B. subtilis FtsZ (366-382); we provided evidence indicating that SepF binds primarily to the C-terminal tail of FtsZ. The present work in concert with the available in vivo data support a model in which SepF plays an important role in regulating the assembly dynamics of the divisome complex; therefore, it may have an important role in bacterial cell division." @default.
• W2087675721 created "2016-06-24" @default.
• W2087675721 creator A5022942658 @default.
• W2087675721 creator A5026724807 @default.
• W2087675721 creator A5029782832 @default.
• W2087675721 creator A5083513744 @default.
• W2087675721 date "2008-11-01" @default.
• W2087675721 modified "2023-10-13" @default.
• W2087675721 title "SepF Increases the Assembly and Bundling of FtsZ Polymers and Stabilizes FtsZ Protofilaments by Binding along Its Length" @default.
• W2087675721 cites W1505479679 @default.
• W2087675721 cites W1713883573 @default.
• W2087675721 cites W1967936154 @default.
• W2087675721 cites W1968425270 @default.
• W2087675721 cites W1970870025 @default.
• W2087675721 cites W1976293898 @default.
• W2087675721 cites W1989419578 @default.
• W2087675721 cites W2002027065 @default.
• W2087675721 cites W2008228685 @default.
• W2087675721 cites W2015483172 @default.
• W2087675721 cites W2018291538 @default.
• W2087675721 cites W2025109401 @default.
• W2087675721 cites W2040676542 @default.
• W2087675721 cites W2050339362 @default.
• W2087675721 cites W2052749821 @default.
• W2087675721 cites W2054064016 @default.
• W2087675721 cites W2058724809 @default.
• W2087675721 cites W2063535784 @default.
• W2087675721 cites W2070067221 @default.
• W2087675721 cites W2075646389 @default.
• W2087675721 cites W2078842336 @default.
• W2087675721 cites W2088383173 @default.
• W2087675721 cites W2099393783 @default.
• W2087675721 cites W2106107052 @default.
• W2087675721 cites W2110743190 @default.
• W2087675721 cites W2113818747 @default.
• W2087675721 cites W2115064050 @default.
• W2087675721 cites W2120004588 @default.
• W2087675721 cites W2120007451 @default.
• W2087675721 cites W2121464522 @default.
• W2087675721 cites W2122259563 @default.
• W2087675721 cites W2123050792 @default.
• W2087675721 cites W2124514849 @default.
• W2087675721 cites W2125237429 @default.
• W2087675721 cites W2142779706 @default.
• W2087675721 cites W2144497462 @default.
• W2087675721 cites W2168832644 @default.
• W2087675721 cites W2172060567 @default.
• W2087675721 cites W4293247451 @default.
• W2087675721 doi "https://doi.org/10.1074/jbc.m805910200" @default.
• W2087675721 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2662178" @default.
• W2087675721 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/18782755" @default.
• W2087675721 hasPublicationYear "2008" @default.
• W2087675721 type Work @default.
• W2087675721 sameAs 2087675721 @default.
• W2087675721 citedByCount "80" @default.
• W2087675721 countsByYear W20876757212012 @default.
• W2087675721 countsByYear W20876757212013 @default.
• W2087675721 countsByYear W20876757212014 @default.
• W2087675721 countsByYear W20876757212015 @default.
• W2087675721 countsByYear W20876757212016 @default.
• W2087675721 countsByYear W20876757212017 @default.
• W2087675721 countsByYear W20876757212018 @default.
• W2087675721 countsByYear W20876757212019 @default.
• W2087675721 countsByYear W20876757212020 @default.
• W2087675721 countsByYear W20876757212021 @default.
• W2087675721 countsByYear W20876757212022 @default.
• W2087675721 countsByYear W20876757212023 @default.
• W2087675721 crossrefType "journal-article" @default.
• W2087675721 hasAuthorship W2087675721A5022942658 @default.
• W2087675721 hasAuthorship W2087675721A5026724807 @default.
• W2087675721 hasAuthorship W2087675721A5029782832 @default.
• W2087675721 hasAuthorship W2087675721A5083513744 @default.
• W2087675721 hasBestOaLocation W20876757211 @default.
• W2087675721 hasConcept C104317684 @default.
• W2087675721 hasConcept C12554922 @default.
• W2087675721 hasConcept C133571119 @default.
• W2087675721 hasConcept C142669718 @default.
• W2087675721 hasConcept C1491633281 @default.
• W2087675721 hasConcept C185592680 @default.
• W2087675721 hasConcept C20418707 @default.
• W2087675721 hasConcept C207332259 @default.
• W2087675721 hasConcept C2776237524 @default.
• W2087675721 hasConcept C2777272437 @default.
• W2087675721 hasConcept C523546767 @default.
• W2087675721 hasConcept C54355233 @default.
• W2087675721 hasConcept C547475151 @default.
• W2087675721 hasConcept C55493867 @default.
• W2087675721 hasConcept C84425145 @default.
• W2087675721 hasConcept C85813293 @default.
• W2087675721 hasConcept C86803240 @default.
• W2087675721 hasConcept C95444343 @default.
• W2087675721 hasConceptScore W2087675721C104317684 @default.
• W2087675721 hasConceptScore W2087675721C12554922 @default.
• W2087675721 hasConceptScore W2087675721C133571119 @default.
• W2087675721 hasConceptScore W2087675721C142669718 @default.
• W2087675721 hasConceptScore W2087675721C1491633281 @default.
• W2087675721 hasConceptScore W2087675721C185592680 @default.
• W2087675721 hasConceptScore W2087675721C20418707 @default.

• next