FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2087776976> ?p ?o ?g. }

• W2087776976 abstract "Inhibitor of DNA binding/Differentiation 1 (ID1) is a helix loop helix transcription factor that lacks the basic DNA binding domain. Over-expression of ID1 has been correlated with a variety of human cancers; our earlier studies had shown that reported ID1 is induced by nicotine or EGF stimulation of non-small cell lung cancer (NSCLC) cells and its down regulation abrogates cell proliferation, invasion and migration. Here we made attempts to identify downstream targets of ID1 that mediate these effects. A microarray analysis was done on two different NSCLC cell lines (A549 and H1650) that were transfected with a siRNA to ID1 or a control, non-targeting siRNA. Cells were stimulated with nicotine and genes that were differentially expressed upon nicotine stimulation and ID1 depletion were analyzed to identify potential downstream targets of ID1. The prospective role of the identified genes was validated by RT-PCR. Additional functional assays were conducted to assess the role of these genes in nicotine induced proliferation, invasion and migration. Experiments were also conducted to elucidate the role of ID1, which does not bind to DNA directly, affects the expression of these genes at transcriptional level. A microarray analysis showed multiple genes are affected by the depletion of ID1; we focused on two of them: Stathmin-like3 (STMN3), a microtubule destabilizing protein, and GSPT1, a protein involved in translation termination; these proteins were induced by both nicotine and EGF in an ID1 dependent fashion. Overexpression of ID1 in two different cell lines induced STMN3 and GSPT1 at the transcriptional level, while depletion of ID1 reduced their expression. STMN3 and GSPT1 were found to facilitate the proliferation, invasion and migration of NSCLC cells in response to nAChR activation. Attempts made to assess how ID1, which is a transcriptional repressor, induces these genes showed that ID1 down regulates the expression of two transcriptional co-repressors, NRSF and ZBP89, involved in the repression of these genes. Collectively, our data suggests that nicotine and EGF induce genes such as STMN3 and GSPT1 to promote the proliferation, invasion and migration of NSCLC, thus enhancing their tumorigenic properties. These studies thus reveal a central role for ID1 and its downstream targets in facilitating lung cancer progression." @default.
• W2087776976 created "2016-06-24" @default.
• W2087776976 creator A5006881843 @default.
• W2087776976 creator A5028119244 @default.
• W2087776976 creator A5028727643 @default.
• W2087776976 creator A5059978666 @default.
• W2087776976 date "2014-07-16" @default.
• W2087776976 modified "2023-10-16" @default.
• W2087776976 title "Nicotine-mediated invasion and migration of non-small cell lung carcinoma cells by modulating STMN3 and GSPT1 genes in an ID1-dependent manner" @default.
• W2087776976 cites W1214585372 @default.
• W2087776976 cites W1603203793 @default.
• W2087776976 cites W1967483950 @default.
• W2087776976 cites W1971166256 @default.
• W2087776976 cites W1977393878 @default.
• W2087776976 cites W1996027776 @default.
• W2087776976 cites W1997058261 @default.
• W2087776976 cites W1998436299 @default.
• W2087776976 cites W2012354846 @default.
• W2087776976 cites W2024285999 @default.
• W2087776976 cites W2025033168 @default.
• W2087776976 cites W2028032755 @default.
• W2087776976 cites W2031281101 @default.
• W2087776976 cites W2036647569 @default.
• W2087776976 cites W2042735215 @default.
• W2087776976 cites W2049714385 @default.
• W2087776976 cites W2055043387 @default.
• W2087776976 cites W2056102602 @default.
• W2087776976 cites W2056548559 @default.
• W2087776976 cites W2061389335 @default.
• W2087776976 cites W2069140606 @default.
• W2087776976 cites W2073477066 @default.
• W2087776976 cites W2090904720 @default.
• W2087776976 cites W2091222325 @default.
• W2087776976 cites W2100622605 @default.
• W2087776976 cites W2101324933 @default.
• W2087776976 cites W2104838343 @default.
• W2087776976 cites W2105405705 @default.
• W2087776976 cites W2107791134 @default.
• W2087776976 cites W2108066901 @default.
• W2087776976 cites W2122975754 @default.
• W2087776976 cites W2125195105 @default.
• W2087776976 cites W2126005969 @default.
• W2087776976 cites W2130883886 @default.
• W2087776976 cites W2132101097 @default.
• W2087776976 cites W2134596328 @default.
• W2087776976 cites W2136373954 @default.
• W2087776976 cites W2137097299 @default.
• W2087776976 cites W2153527917 @default.
• W2087776976 cites W2159511823 @default.
• W2087776976 cites W2161817637 @default.
• W2087776976 cites W2162478991 @default.
• W2087776976 cites W2163505622 @default.
• W2087776976 cites W2170978255 @default.
• W2087776976 cites W2172326429 @default.
• W2087776976 doi "https://doi.org/10.1186/1476-4598-13-173" @default.
• W2087776976 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4121302" @default.
• W2087776976 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/25028095" @default.
• W2087776976 hasPublicationYear "2014" @default.
• W2087776976 type Work @default.
• W2087776976 sameAs 2087776976 @default.
• W2087776976 citedByCount "27" @default.
• W2087776976 countsByYear W20877769762015 @default.
• W2087776976 countsByYear W20877769762016 @default.
• W2087776976 countsByYear W20877769762017 @default.
• W2087776976 countsByYear W20877769762018 @default.
• W2087776976 countsByYear W20877769762019 @default.
• W2087776976 countsByYear W20877769762020 @default.
• W2087776976 countsByYear W20877769762021 @default.
• W2087776976 countsByYear W20877769762022 @default.
• W2087776976 countsByYear W20877769762023 @default.
• W2087776976 crossrefType "journal-article" @default.
• W2087776976 hasAuthorship W2087776976A5006881843 @default.
• W2087776976 hasAuthorship W2087776976A5028119244 @default.
• W2087776976 hasAuthorship W2087776976A5028727643 @default.
• W2087776976 hasAuthorship W2087776976A5059978666 @default.
• W2087776976 hasBestOaLocation W20877769761 @default.
• W2087776976 hasConcept C104317684 @default.
• W2087776976 hasConcept C150194340 @default.
• W2087776976 hasConcept C153911025 @default.
• W2087776976 hasConcept C2778654611 @default.
• W2087776976 hasConcept C502942594 @default.
• W2087776976 hasConcept C54009773 @default.
• W2087776976 hasConcept C54355233 @default.
• W2087776976 hasConcept C62112901 @default.
• W2087776976 hasConcept C81885089 @default.
• W2087776976 hasConcept C8415881 @default.
• W2087776976 hasConcept C86339819 @default.
• W2087776976 hasConcept C86803240 @default.
• W2087776976 hasConcept C95444343 @default.
• W2087776976 hasConceptScore W2087776976C104317684 @default.
• W2087776976 hasConceptScore W2087776976C150194340 @default.
• W2087776976 hasConceptScore W2087776976C153911025 @default.
• W2087776976 hasConceptScore W2087776976C2778654611 @default.
• W2087776976 hasConceptScore W2087776976C502942594 @default.
• W2087776976 hasConceptScore W2087776976C54009773 @default.
• W2087776976 hasConceptScore W2087776976C54355233 @default.
• W2087776976 hasConceptScore W2087776976C62112901 @default.
• W2087776976 hasConceptScore W2087776976C81885089 @default.
• W2087776976 hasConceptScore W2087776976C8415881 @default.
• W2087776976 hasConceptScore W2087776976C86339819 @default.

• next