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• W2087813993 abstract "You have accessJournal of UrologyTransplantation & Vascular Surgery: Renal Transplantation, Vascular Surgery II1 Apr 20102171 RENAL TRANSPLANT PROTECTION USING PERFUSATE-BASED CARBON MONOXIDE RELEASING MOLECULES Patrick Luke, Jian Deng, Hao Wang, Hong Tao Sun, Anthony Jevnikar, and Gediminas Cepinskas Patrick LukePatrick Luke More articles by this author , Jian DengJian Deng More articles by this author , Hao WangHao Wang More articles by this author , Hong Tao SunHong Tao Sun More articles by this author , Anthony JevnikarAnthony Jevnikar More articles by this author , and Gediminas CepinskasGediminas Cepinskas More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2010.02.2274AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES Carbon monoxide (CO) has previously been shown to reduce damage associated with ischemia reperfusion injury, but is difficult to store and deliver CO in a safe, controlled manner. Therefore, we assessed the ability of novel carbon monoxide releasing molecules (CORM) to prevent graft injury as well as endothelial and epithelial apoptosis. METHODS To assess the ability of CORM-3 to protect human tubular epithelial cell (TEC) and human umbilical vein endothelial cell (HUVEC) from cytokine-mediated injury, TEC were incubated in 10 ng/ml TNF-a and IFN-g for 24 hr. As well, cells were incubated in an anoxia/reoxygenation chamber. In parallel, isogeneic Lewis to Lewis renal transplants were performed with a 26 hr cold ischemic time. Group 1 animals had 100 microM CORM-3 infusion into the graft at the time of retrieval and prior to implantation along with 26 hr cold storage in University of Wisconsin solution (UW) (n = 6). Group 2 animals (n = 6) were stored for 26 hr in cold UW solution. RESULTS Using Annexin V/ 7-AAD staining, cell death was shown to be reduced in both TEC and HUVEC after pre-incubation in CORM-3 in a concentration-dependent manner with optimal protection at 100 microM of CORM-3. Pre-incubation in inactivated CORM (iCORM-3) failed to offer protection. Five of 6 recipients that received CORM-3 isografts survived the transplant process, whereas all control animals died by day 3 post-operatively. The mean serum creatinine (sCr) in Groups 1 and 2 were 191+/- 224 and 680+/-15 umol/L by 72 hr, respectively (p<0.05). Early post-operative histology revealed that CORM-3-treated grafts suffered mild acute tubular necrosis whereas controls experienced severe injury. CONCLUSIONS In summary, we have shown that perfusate-infused CORM-3 protects the renal transplant and prevents apoptosis of relevant epithelial and endothelial cells from anoxia/reoxygenation and inflammation-related injury. This provides rationale to use CORM in transplant perfusate solutions to protect the organ during cold storage and reperfusion. London, Canada© 2010 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 183Issue 4SApril 2010Page: e845 Advertisement Copyright & Permissions© 2010 by American Urological Association Education and Research, Inc.MetricsAuthor Information Patrick Luke More articles by this author Jian Deng More articles by this author Hao Wang More articles by this author Hong Tao Sun More articles by this author Anthony Jevnikar More articles by this author Gediminas Cepinskas More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ..." @default.
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• W2087813993 date "2010-04-01" @default.
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• W2087813993 title "2171 RENAL TRANSPLANT PROTECTION USING PERFUSATE-BASED CARBON MONOXIDE RELEASING MOLECULES" @default.
• W2087813993 doi "https://doi.org/10.1016/j.juro.2010.02.2274" @default.
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