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• W2087815706 abstract "Ultraviolet-induced DNA repair synthesis was measured in saponin-permeabilized normal human fibroblasts by the incorporation of [alpha-32P]dTMP into DNA. The involvement of DNA polymerases alpha, beta, delta, and epsilon in excision repair of pyrimidine dimers was examined using specific inhibitors. Dose-response curves resulting from experiments with up to 12 different inhibitor concentrations were analyzed by linear regression. Inhibitor concentrations at which repair activity was reduced to 50% were calculated. The following K50 values were found: aphidicolin, 0.2 microM; ddTTP, 12.5 microM; butylphenyl-dGTP, 7.6 microM; butylanilino-dATP, 6.0 microM. Comparison of K50 values with in vitro Ki values of DNA polymerases revealed that in permeabilized human fibroblasts reparative DNA synthesis is catalyzed by DNA polymerase delta and by DNA polymerase epsilon." @default.
• W2087815706 created "2016-06-24" @default.
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• W2087815706 date "1992-01-01" @default.
• W2087815706 modified "2023-10-16" @default.
• W2087815706 title "The function of DNA polymerases in DNA repair synthesis of ultraviolet-irradiated human fibroblasts" @default.
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• W2087815706 doi "https://doi.org/10.1016/0167-4781(92)90480-n" @default.
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