FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2087889505> ?p ?o ?g. }

• W2087889505 endingPage "19123" @default.
• W2087889505 startingPage "19118" @default.
• W2087889505 abstract "The B subunits of Escherichia coli heat-labile enterotoxin (EtxB) and cholera toxin (CtxB) assemble in vivo into exceptionally stable homopentameric complexes, which maintain their quaternary structure in a range of conditions that would normally be expected to cause protein denaturation. Recently, we showed that the simultaneous protonation of two of the COOH-terminal carboxylates in pentameric EtxB was required to cause its disassembly at pH values below 2.0 (Ruddock, L., Ruston, S. P., Kelly, S. M., Price, N. C., Freedman, R. B., and Hirst, T. R.(1995) J. Biol. Chem. 270, 29953-29958). Here, we investigate the influence of environmental parameters on the kinetics of reassembly of acid-generated EtxB monomers in vitro. Such monomers were found to undergo a further acid-mediated conformational change, with an activation energy of 76 +/- 2 J.mol-1.K-1, consistent with isomerization of the cis-proline residue at position 93, and which prevented subsequent EtxB reassembly. By using rapid neutralization of acid-generated monomers, a high proportion of the B-subunits adopted an assembly-competent conformation, which resulted in up to 75% of the protein reassembling into a stable pentameric complex, indistinguishable from native EtxB pentamers. The rate-limiting step in reassembly, over a concentration range of 50-200 microg/ml, was shown to be due to an intramolecular event, which exhibited a pH dependence with a pKa of 7.0. Modification of EtxB with amine-specific probes revealed that the protonation state of the NH2-terminal alanine residue was responsible for the pH dependence of reassembly. The implications of these findings for the biogenesis of Escherichia coli enterotoxin and related enterotoxins in vivo, are considered." @default.
• W2087889505 created "2016-06-24" @default.
• W2087889505 creator A5016746605 @default.
• W2087889505 creator A5030210449 @default.
• W2087889505 creator A5049252208 @default.
• W2087889505 creator A5076271698 @default.
• W2087889505 creator A5087597179 @default.
• W2087889505 date "1996-08-01" @default.
• W2087889505 modified "2023-09-26" @default.
• W2087889505 title "Assembly of the B Subunit Pentamer of Escherichia coli Heat-labile Enterotoxin" @default.
• W2087889505 cites W1488068074 @default.
• W2087889505 cites W1503431398 @default.
• W2087889505 cites W1513651808 @default.
• W2087889505 cites W1514909941 @default.
• W2087889505 cites W1571215450 @default.
• W2087889505 cites W1617179398 @default.
• W2087889505 cites W1833333324 @default.
• W2087889505 cites W1974972571 @default.
• W2087889505 cites W1987155125 @default.
• W2087889505 cites W1992292801 @default.
• W2087889505 cites W2007165330 @default.
• W2087889505 cites W2014540222 @default.
• W2087889505 cites W2017561569 @default.
• W2087889505 cites W2017639307 @default.
• W2087889505 cites W2037729661 @default.
• W2087889505 cites W2049659921 @default.
• W2087889505 cites W2050869281 @default.
• W2087889505 cites W2051791661 @default.
• W2087889505 cites W2062265953 @default.
• W2087889505 cites W2067359698 @default.
• W2087889505 cites W2078733777 @default.
• W2087889505 cites W2084179622 @default.
• W2087889505 cites W2094245705 @default.
• W2087889505 cites W2096689233 @default.
• W2087889505 cites W2115338359 @default.
• W2087889505 cites W2124864045 @default.
• W2087889505 cites W2139357859 @default.
• W2087889505 cites W2144305638 @default.
• W2087889505 cites W2156782507 @default.
• W2087889505 cites W2211987955 @default.
• W2087889505 cites W4211185542 @default.
• W2087889505 doi "https://doi.org/10.1074/jbc.271.32.19118" @default.
• W2087889505 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8702586" @default.
• W2087889505 hasPublicationYear "1996" @default.
• W2087889505 type Work @default.
• W2087889505 sameAs 2087889505 @default.
• W2087889505 citedByCount "25" @default.
• W2087889505 countsByYear W20878895052012 @default.
• W2087889505 countsByYear W20878895052013 @default.
• W2087889505 countsByYear W20878895052014 @default.
• W2087889505 countsByYear W20878895052015 @default.
• W2087889505 countsByYear W20878895052016 @default.
• W2087889505 countsByYear W20878895052017 @default.
• W2087889505 countsByYear W20878895052018 @default.
• W2087889505 countsByYear W20878895052020 @default.
• W2087889505 countsByYear W20878895052021 @default.
• W2087889505 crossrefType "journal-article" @default.
• W2087889505 hasAuthorship W2087889505A5016746605 @default.
• W2087889505 hasAuthorship W2087889505A5030210449 @default.
• W2087889505 hasAuthorship W2087889505A5049252208 @default.
• W2087889505 hasAuthorship W2087889505A5076271698 @default.
• W2087889505 hasAuthorship W2087889505A5087597179 @default.
• W2087889505 hasBestOaLocation W20878895051 @default.
• W2087889505 hasConcept C104317684 @default.
• W2087889505 hasConcept C145148216 @default.
• W2087889505 hasConcept C166940927 @default.
• W2087889505 hasConcept C178790620 @default.
• W2087889505 hasConcept C185592680 @default.
• W2087889505 hasConcept C2777331810 @default.
• W2087889505 hasConcept C2778353597 @default.
• W2087889505 hasConcept C30095370 @default.
• W2087889505 hasConcept C521977710 @default.
• W2087889505 hasConcept C547475151 @default.
• W2087889505 hasConcept C55493867 @default.
• W2087889505 hasConcept C66999527 @default.
• W2087889505 hasConcept C71240020 @default.
• W2087889505 hasConceptScore W2087889505C104317684 @default.
• W2087889505 hasConceptScore W2087889505C145148216 @default.
• W2087889505 hasConceptScore W2087889505C166940927 @default.
• W2087889505 hasConceptScore W2087889505C178790620 @default.
• W2087889505 hasConceptScore W2087889505C185592680 @default.
• W2087889505 hasConceptScore W2087889505C2777331810 @default.
• W2087889505 hasConceptScore W2087889505C2778353597 @default.
• W2087889505 hasConceptScore W2087889505C30095370 @default.
• W2087889505 hasConceptScore W2087889505C521977710 @default.
• W2087889505 hasConceptScore W2087889505C547475151 @default.
• W2087889505 hasConceptScore W2087889505C55493867 @default.
• W2087889505 hasConceptScore W2087889505C66999527 @default.
• W2087889505 hasConceptScore W2087889505C71240020 @default.
• W2087889505 hasIssue "32" @default.
• W2087889505 hasLocation W20878895051 @default.
• W2087889505 hasLocation W20878895052 @default.
• W2087889505 hasOpenAccess W2087889505 @default.
• W2087889505 hasPrimaryLocation W20878895051 @default.
• W2087889505 hasRelatedWork W1496765254 @default.
• W2087889505 hasRelatedWork W1886652222 @default.
• W2087889505 hasRelatedWork W1918072707 @default.
• W2087889505 hasRelatedWork W1958187136 @default.

• next