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- W2087906543 abstract "Microsomal membranes prepared from the mesophilic yeast Candida lipolytica grown at 10 °C were hydrogenated by the homogeneous Pd-catalyst, palladium di(sodium alizarine sulfonate) (Pd(QS)2). After hydrogenation to various levels, the microsomes were washed free of the Pd-complex and transferred to a reaction mixture (containing NADH, MgCl2, ATP, CoA and [14C]18:1-CoA) for assay of 18:1-CoA desaturase activity. Microviscosity alterations were also followed by measuring changes in DPH fluorescence polarization. Rapid catalytic hydrogenation of unsaturated fatty acids of the lipids occurred within 20–120 s, resulting in large increases in 16:0, 18:0 and 18:1 acids and decreases in 18:2 acid. In the range 7–20% 18:0 content, a pronounced increase in desaturase activity was observed, with a maximum of > 2-fold at a 18:0 content of 12%, followed by a decrease to the initial activity at 33% 18:0 content. These changes were well-correlated with changes in microviscosity, maximal desaturase activity occurring in the DPH fluorescence anisotropy range of 0.23–0.24; above and below this range, desaturase activities were close to the initial control values. It is suggested that the hydrogenation-induced increase in the formation of 18:2 from 18:1-CoA (proceeding partly through direct desaturation of PC) may be due to changes in conformation of the membrane-bound desaturase enzyme complex as a result of controlled rigidification of the surrounding lipids. The operation of such a self-regulating control mechanism would be consistent with a previously proposed model for microsomal desaturase action." @default.
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- W2087906543 date "1991-08-01" @default.
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- W2087906543 title "Lipid hydrogenation induces elevated 18:1-CoA desaturase activity in Candida lipolytica microsomes" @default.
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- W2087906543 doi "https://doi.org/10.1016/0005-2760(91)90240-i" @default.
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