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- W2087932827 abstract "Abstract The separation of outer and cytoplasmic membrane fractions has been difficult to achieve with pseudomonads. A new procedure is described in which Pseudomonas facilis is treated with methyl picolinimidate, subsequently with buffered sucrose, and finally with lysozyme-EDTA to yield spheroplasts; spheroplast-derived envelopes can then be resolved by density gradient centrifugation into several components only one of which is more dense in the presence of Ni2+. This latter component, corresponding to the cell wall fraction, contains about 2% of the succinate dehydrogenase, a cytoplasmic membrane marker. Analogous experiments with erythrocytes establish that spectrin, a protein known to be located on the inner membrane surface, is not labeled by the methyl picolinimidate." @default.
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- W2087932827 date "1977-12-01" @default.
- W2087932827 modified "2023-10-16" @default.
- W2087932827 title "Bacterial and erythrocyte cell surface labeling by methyl picolinimidate" @default.
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- W2087932827 doi "https://doi.org/10.1016/0003-2697(77)90053-7" @default.
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