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- W2087950712 abstract "The incidence of primary dysfunction or non-function of liver grafts still occurs at an unacceptable rate and the identification of new markers of graft viability and metabolic capacity is essential. Proton nuclear magnetic resonance (H NMR) spectroscopy has previously shown potential in the evaluation of renal allograft dysfunction after ischaemia reperfusion. The aim of this study was to compare liver graft function in a rat isolated perfused liver model after 24 hours of preservation in either Euro-Collins (EC) or University of Wisconsin (UW) solutions. Livers were reperfused for 90 min with a modified Krebs-Henseleit medium. Functional parameters measured were: pressure and resistances, bile and transaminase production. The production of lactate, pyruvate, citrate and succinate, beta-hydroxybutyrate and aceto-acetate was measured by H NMR. There was a significant difference in both haemodynamics and bile production in favour of the UW group. The transaminases were similar in the two groups. Measurements of citrate, succinate and ketone bodies by H NMR were all higher in the UW group. These markers indicate better metabolic function of these grafts during reperfusion. In conclusion, discriminating different hepatic metabolic pathways is possible and easy by H NMR and can be used to assess both the preservation quality of liver grafts and their functional recovery." @default.
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- W2087950712 date "2000-01-12" @default.
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- W2087950712 title "A New Approach to the Evaluation of Liver Graft Function by Nuclear Magnetic Resonance Spectroscopy. A Comparative Study between Euro-Collins and University of Wisconsin Solutions" @default.
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- W2087950712 doi "https://doi.org/10.1515/cclm.2000.171" @default.
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