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• W2087952364 endingPage "288" @default.
• W2087952364 startingPage "259" @default.
• W2087952364 abstract "The many techniques used to diagnose the Factor V Leiden (FVL) mutation, the most common hereditary hypercoagulation disorder in Eurasians, and the most frequently requested genetic test reflect the evolving strategies in protein and DNA diagnosis.Here, molecular methods to diagnose the FVL mutation are discussed.Protein-based detection assays include the conventional functional activated protein C resistance coagulation test and the recently reported antibody-mediated sensor detection; and DNA-based assays include approaches that use electrophoretic fractionation e.g., restriction fragment length polymorphism, denaturing gradient gel electrophoresis, and single-stranded conformational PCR analysis, DNA hybridization (e.g., microarrays), DNA polymerase-based assays, e.g., extension reactions, fluorescence polarization template-directed dye-terminator incorporation, PCR assays (e.g., amplification-refractory mutation system, melting curve analysis using real-time quantitative PCR, and helicase-dependent amplification), DNA sequencing (e.g., direct sequencing, pyrosequencing), cleavase-based Invader assay and ligase-based assays (e.g., oligonucleotide ligation assay and ligase-mediated rolling circle amplification).The method chosen by a laboratory to diagnose FVL not only depends on the available technical expertise and equipment, but also the type, variety, and extent of other genetic disorders being diagnosed." @default.
• W2087952364 created "2016-06-24" @default.
• W2087952364 creator A5028789394 @default.
• W2087952364 creator A5090045378 @default.
• W2087952364 date "2011-01-01" @default.
• W2087952364 modified "2023-10-10" @default.
• W2087952364 title "Evolving methods for single nucleotide polymorphism detection: Factor V Leiden mutation detection" @default.
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• W2087952364 doi "https://doi.org/10.1002/jcla.20470" @default.
• W2087952364 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6647683" @default.
• W2087952364 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/21786330" @default.
• W2087952364 hasPublicationYear "2011" @default.
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