FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2088047164> ?p ?o ?g. }

• W2088047164 endingPage "52" @default.
• W2088047164 startingPage "46" @default.
• W2088047164 abstract "To develop a new stationary phase of easy production, low cost, biocompatible, biodegradable and low unspecific adsorption, a three-dimensional network was prepared by combining the natural polysaccharide of gellan with divalent cations. The stability of this cation exchange chromatographic matrix was optimized by using an experimental design tool. The optimal conditions proposed for the gellan gel formulation were 48mM ZnSO4, 0% DMF, 25°C, 0.75% gellan and 0.5h. The applicability of gellan matrix was tested by chromatographic assays with three model proteins (bovine serum albumin (BSA), α-chymotripsin and lysozyme). The results showed that the retention occurred in function of the net charge of each protein in MES buffer pH 6.2 and the elution was performed by increase of ionic strength to 750mM NaCl in MES buffer pH 6.2. Lysozyme was the more retained protein due to its positive charge more effective than α-chymotripsin, while BSA did not interact with the matrix due to its negative charge at these conditions. Dynamic binding capacity assays were accomplished to characterize this matrix and to compare with commercial resins. The values of dynamic binding capacity from gellan gel were 3.9mg/mL and 17.4mg/mL, at 10% and 50% of breakthrough, respectively. In this way, gellan gel might be a promising chromatographic matrix to explore ionic interactions and to be applied in different purification strategies, getting the best benefit from its use at low cost." @default.
• W2088047164 created "2016-06-24" @default.
• W2088047164 creator A5001094739 @default.
• W2088047164 creator A5013768148 @default.
• W2088047164 creator A5047892371 @default.
• W2088047164 creator A5049252324 @default.
• W2088047164 creator A5076805164 @default.
• W2088047164 date "2014-04-01" @default.
• W2088047164 modified "2023-09-27" @default.
• W2088047164 title "Optimization of a chromatographic stationary phase based on gellan gum using central composite design" @default.
• W2088047164 cites W10085712 @default.
• W2088047164 cites W1535514846 @default.
• W2088047164 cites W1871966943 @default.
• W2088047164 cites W1967246723 @default.
• W2088047164 cites W1972511084 @default.
• W2088047164 cites W1976361279 @default.
• W2088047164 cites W1977079554 @default.
• W2088047164 cites W1985926545 @default.
• W2088047164 cites W1989498696 @default.
• W2088047164 cites W2001311709 @default.
• W2088047164 cites W2003449739 @default.
• W2088047164 cites W2017033154 @default.
• W2088047164 cites W2017924132 @default.
• W2088047164 cites W2021993140 @default.
• W2088047164 cites W2033494270 @default.
• W2088047164 cites W2039431423 @default.
• W2088047164 cites W2041975070 @default.
• W2088047164 cites W2047532550 @default.
• W2088047164 cites W2050580961 @default.
• W2088047164 cites W2051657448 @default.
• W2088047164 cites W2052634646 @default.
• W2088047164 cites W2056533312 @default.
• W2088047164 cites W2062618848 @default.
• W2088047164 cites W2065854055 @default.
• W2088047164 cites W2066561369 @default.
• W2088047164 cites W2067049506 @default.
• W2088047164 cites W2068543266 @default.
• W2088047164 cites W2085391320 @default.
• W2088047164 cites W2088593140 @default.
• W2088047164 cites W2089923614 @default.
• W2088047164 cites W2093630016 @default.
• W2088047164 cites W2107116365 @default.
• W2088047164 cites W2114118213 @default.
• W2088047164 cites W2141575961 @default.
• W2088047164 cites W2315558541 @default.
• W2088047164 cites W47554775 @default.
• W2088047164 doi "https://doi.org/10.1016/j.jchromb.2014.02.050" @default.
• W2088047164 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/24657410" @default.
• W2088047164 hasPublicationYear "2014" @default.
• W2088047164 type Work @default.
• W2088047164 sameAs 2088047164 @default.
• W2088047164 citedByCount "6" @default.
• W2088047164 countsByYear W20880471642017 @default.
• W2088047164 countsByYear W20880471642018 @default.
• W2088047164 countsByYear W20880471642019 @default.
• W2088047164 countsByYear W20880471642021 @default.
• W2088047164 countsByYear W20880471642022 @default.
• W2088047164 crossrefType "journal-article" @default.
• W2088047164 hasAuthorship W2088047164A5001094739 @default.
• W2088047164 hasAuthorship W2088047164A5013768148 @default.
• W2088047164 hasAuthorship W2088047164A5047892371 @default.
• W2088047164 hasAuthorship W2088047164A5049252324 @default.
• W2088047164 hasAuthorship W2088047164A5076805164 @default.
• W2088047164 hasConcept C106487976 @default.
• W2088047164 hasConcept C150394285 @default.
• W2088047164 hasConcept C178790620 @default.
• W2088047164 hasConcept C184651966 @default.
• W2088047164 hasConcept C185592680 @default.
• W2088047164 hasConcept C187428577 @default.
• W2088047164 hasConcept C2776966407 @default.
• W2088047164 hasConcept C2781220964 @default.
• W2088047164 hasConcept C31903555 @default.
• W2088047164 hasConcept C43617362 @default.
• W2088047164 hasConcept C44280652 @default.
• W2088047164 hasConcept C55493867 @default.
• W2088047164 hasConcept C73826308 @default.
• W2088047164 hasConcept C74884574 @default.
• W2088047164 hasConcept C86807702 @default.
• W2088047164 hasConceptScore W2088047164C106487976 @default.
• W2088047164 hasConceptScore W2088047164C150394285 @default.
• W2088047164 hasConceptScore W2088047164C178790620 @default.
• W2088047164 hasConceptScore W2088047164C184651966 @default.
• W2088047164 hasConceptScore W2088047164C185592680 @default.
• W2088047164 hasConceptScore W2088047164C187428577 @default.
• W2088047164 hasConceptScore W2088047164C2776966407 @default.
• W2088047164 hasConceptScore W2088047164C2781220964 @default.
• W2088047164 hasConceptScore W2088047164C31903555 @default.
• W2088047164 hasConceptScore W2088047164C43617362 @default.
• W2088047164 hasConceptScore W2088047164C44280652 @default.
• W2088047164 hasConceptScore W2088047164C55493867 @default.
• W2088047164 hasConceptScore W2088047164C73826308 @default.
• W2088047164 hasConceptScore W2088047164C74884574 @default.
• W2088047164 hasConceptScore W2088047164C86807702 @default.
• W2088047164 hasLocation W20880471641 @default.
• W2088047164 hasLocation W20880471642 @default.
• W2088047164 hasOpenAccess W2088047164 @default.
• W2088047164 hasPrimaryLocation W20880471641 @default.
• W2088047164 hasRelatedWork W2005165146 @default.

• next