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- W2088122764 abstract "Replicating, circular chromosomes of Bacillus subtilis have been displayed by autoradiography of samples obtained from thymine-requiring spores germinating in the presence of [3H]thymine and proceeding into the second round of replication. The observed chromosomes have the expected Cairns-type configuration, and the measured length of a completed (non-replicating) chromosome is 900 to 1100 μm. The extent to which bidirectional replication can proceed following initiation of a round has been investigated further. Thymine-requiring spores germinating in the presence of [3H]thymidine were diluted into higher specific activity medium during the first round of replication and autoradiographs prepared from samples obtained shortly afterwards. From the pattern of labelling within individual loops that become visible, it is clear that replication can proceed in a bidirectional manner up to a stage that would account for replication of at least 50% of the whole chromosome. And, until this stage, the replication rates in both directions are approximately equal." @default.
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- W2088122764 date "1973-07-01" @default.
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- W2088122764 title "Circularity of the Bacillus subtilis chromosome and further studies on its bidirectional replication" @default.
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- W2088122764 doi "https://doi.org/10.1016/0022-2836(73)90223-4" @default.
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