Matches in SemOpenAlex for { <https://semopenalex.org/work/W2088147822> ?p ?o ?g. }
Showing items 1 to 94 of
94
with 100 items per page.
- W2088147822 endingPage "1305" @default.
- W2088147822 startingPage "1296" @default.
- W2088147822 abstract "The aim of this study was to establish a novel approach for human leukocyte antigen (HLA)-typing from formalin-fixed paraffin-embedded-derived DNA. HLAs can be a prognostic factor in cancer and have an extensive polymorphism. This polymorphism is predominantly restricted to exons, which encode the peptide-binding domain of the protein. Formalin-fixed paraffin-embedded material is routinely collected in the clinic and therefore a great source of DNA for genetic analyses. However, its low quality due to fragmentation and nucleotide changes has often created obstacles in designing genetic assays. In this study, we amplified the most polymorphic exons of the HLA-A gene, exons 2, 3, and 4, in 16 formalin-fixed paraffin-embedded samples >10 years old. These tissue samples belonged to patients already HLA-typed by peripheral blood samples at the routine laboratory. Acquired amplification products were used for sequencing, which provided enough information to establish an HLA allele. The same method was applied to DNA extracted from peripheral blood from a healthy volunteer with known HLA type. Of the samples, 14/16 (88%) were successfully typed, in one sample only one of the alleles could be determined, and in one sample no allele could be determined. The amplification of the most polymorphic exons of HLA-A was a successful alternative when DNA quality prevented positive results with previously described methods. The method is usable when an HLA type is needed but the patients are deceased and/or no whole blood samples can be collected. It has thus potential to be used in several fields such as the clinic, research, and forensic science." @default.
- W2088147822 created "2016-06-24" @default.
- W2088147822 creator A5029462216 @default.
- W2088147822 creator A5043373904 @default.
- W2088147822 creator A5053539722 @default.
- W2088147822 creator A5071060959 @default.
- W2088147822 creator A5073774276 @default.
- W2088147822 creator A5077987319 @default.
- W2088147822 date "2014-09-01" @default.
- W2088147822 modified "2023-10-18" @default.
- W2088147822 title "A novel approach for HLA-A typing in formalin-fixed paraffin-embedded-derived DNA" @default.
- W2088147822 cites W1497954397 @default.
- W2088147822 cites W1967041353 @default.
- W2088147822 cites W1968358961 @default.
- W2088147822 cites W1980094840 @default.
- W2088147822 cites W1984578250 @default.
- W2088147822 cites W1998258610 @default.
- W2088147822 cites W2001014198 @default.
- W2088147822 cites W2022715332 @default.
- W2088147822 cites W2023369529 @default.
- W2088147822 cites W2041140837 @default.
- W2088147822 cites W2053201811 @default.
- W2088147822 cites W2058590427 @default.
- W2088147822 cites W2071726080 @default.
- W2088147822 cites W2073760647 @default.
- W2088147822 cites W2086886569 @default.
- W2088147822 cites W2105636594 @default.
- W2088147822 cites W2106882534 @default.
- W2088147822 cites W2141450265 @default.
- W2088147822 cites W2161183734 @default.
- W2088147822 cites W4239551491 @default.
- W2088147822 cites W84882231 @default.
- W2088147822 doi "https://doi.org/10.1038/modpathol.2013.210" @default.
- W2088147822 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/24504073" @default.
- W2088147822 hasPublicationYear "2014" @default.
- W2088147822 type Work @default.
- W2088147822 sameAs 2088147822 @default.
- W2088147822 citedByCount "5" @default.
- W2088147822 countsByYear W20881478222015 @default.
- W2088147822 countsByYear W20881478222017 @default.
- W2088147822 countsByYear W20881478222020 @default.
- W2088147822 countsByYear W20881478222022 @default.
- W2088147822 crossrefType "journal-article" @default.
- W2088147822 hasAuthorship W2088147822A5029462216 @default.
- W2088147822 hasAuthorship W2088147822A5043373904 @default.
- W2088147822 hasAuthorship W2088147822A5053539722 @default.
- W2088147822 hasAuthorship W2088147822A5071060959 @default.
- W2088147822 hasAuthorship W2088147822A5073774276 @default.
- W2088147822 hasAuthorship W2088147822A5077987319 @default.
- W2088147822 hasBestOaLocation W20881478221 @default.
- W2088147822 hasConcept C104317684 @default.
- W2088147822 hasConcept C147483822 @default.
- W2088147822 hasConcept C153911025 @default.
- W2088147822 hasConcept C17757408 @default.
- W2088147822 hasConcept C180754005 @default.
- W2088147822 hasConcept C188280979 @default.
- W2088147822 hasConcept C2781209916 @default.
- W2088147822 hasConcept C36823959 @default.
- W2088147822 hasConcept C54355233 @default.
- W2088147822 hasConcept C552990157 @default.
- W2088147822 hasConcept C86803240 @default.
- W2088147822 hasConceptScore W2088147822C104317684 @default.
- W2088147822 hasConceptScore W2088147822C147483822 @default.
- W2088147822 hasConceptScore W2088147822C153911025 @default.
- W2088147822 hasConceptScore W2088147822C17757408 @default.
- W2088147822 hasConceptScore W2088147822C180754005 @default.
- W2088147822 hasConceptScore W2088147822C188280979 @default.
- W2088147822 hasConceptScore W2088147822C2781209916 @default.
- W2088147822 hasConceptScore W2088147822C36823959 @default.
- W2088147822 hasConceptScore W2088147822C54355233 @default.
- W2088147822 hasConceptScore W2088147822C552990157 @default.
- W2088147822 hasConceptScore W2088147822C86803240 @default.
- W2088147822 hasIssue "9" @default.
- W2088147822 hasLocation W20881478221 @default.
- W2088147822 hasLocation W20881478222 @default.
- W2088147822 hasOpenAccess W2088147822 @default.
- W2088147822 hasPrimaryLocation W20881478221 @default.
- W2088147822 hasRelatedWork W101503009 @default.
- W2088147822 hasRelatedWork W1960746255 @default.
- W2088147822 hasRelatedWork W22528600 @default.
- W2088147822 hasRelatedWork W2368089815 @default.
- W2088147822 hasRelatedWork W2413704448 @default.
- W2088147822 hasRelatedWork W2888997879 @default.
- W2088147822 hasRelatedWork W4212841426 @default.
- W2088147822 hasRelatedWork W4283775834 @default.
- W2088147822 hasRelatedWork W4367300370 @default.
- W2088147822 hasRelatedWork W4382066202 @default.
- W2088147822 hasVolume "27" @default.
- W2088147822 isParatext "false" @default.
- W2088147822 isRetracted "false" @default.
- W2088147822 magId "2088147822" @default.
- W2088147822 workType "article" @default.