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- W2088215535 abstract "The effects of three membrane-active agents, filipin, digitonin, and polymyxin B on the plasma membrane of ram spermatozoa have been studied by freeze-etch electron microscopy. Filipin and digitonin both reacted with cholesterol and caused visible membrane modification in cholesterol-rich regions, with filipin being a more specific agent than digitonin. Polymyxin B, which is known to interact specifically with negatively charged phospholipids of bacterial membranes, exhibited a selective binding and subsequent modification of sperm plasma membranes. This binding was shown to be inhibited in the presence of 1 mmol/1 Ca2+. We hence propose that both filipin and polymyxin B are useful cytological markers for specific biological molecules in eukaryote membranes--filipin for cholesterol, and polymyxin B for negatively charged phospholipids." @default.
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- W2088215535 date "1980-01-01" @default.
- W2088215535 modified "2023-09-26" @default.
- W2088215535 title "Effects of Filipin, Digitonin, and Polymyxin B on Plasma Membrane of Ram Spermatozoa—An EM Study" @default.
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- W2088215535 doi "https://doi.org/10.3109/01485018008986963" @default.
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