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- W2088291281 abstract "We have developed a sensitive fluorometric assay using bisulfite deaminated (C----U), covalently-closed circular PM2 DNA as the substrate. We describe a reliable way to prepare this substrate without nicking the PM2 DNA. Methods, which depend on toluenization of the cells, are described for reproducibly and quantitatively assaying uracil-DNA glycosylase. The sensitivity is such that only 200 EL4 mouse thymoma cells or 30,000 Escherichia coli cells are needed for each point in a kinetic assay." @default.
- W2088291281 created "2016-06-24" @default.
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- W2088291281 date "1988-02-01" @default.
- W2088291281 modified "2023-09-25" @default.
- W2088291281 title "Quantitative assays for uracil-DNA glycosylase of high sensitivity" @default.
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- W2088291281 doi "https://doi.org/10.1139/o88-021" @default.
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