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- W2088303722 abstract "The dual positional maize lipoxygenase-1 was introduced into rice and T2 transgenic plants were produced. Cellular location of maize lipoxygenase-1 in transgenic rice and effects of calcium ion on membrane association in vitro were analyzed. Localization study by confocal microscopic analysis indicated that the maize lipoxygenase-1 was localized in cytoplasm. Sucrose-density fractionation experiment and in vitro protein transport to chloroplast showed that the maize lipoxygenase-1 can be associated with chloroplast. Secondary structure alignment revealed putative calcium binding sites in the PLAT domain of maize lipoxygenase-1 and the association of the maize lipoxygenase-1 with membranes was mediated by calcium ion in vitro. Our results provide evidences for calcium-mediated translocation of dual positional LOX without chloroplast targeting sequence from cytoplasm to chloroplast in plants for the first time." @default.
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- W2088303722 date "2011-09-01" @default.
- W2088303722 modified "2023-10-16" @default.
- W2088303722 title "Cellular localization of dual positional specific maize lipoxygenase-1 in transgenic rice and calcium-mediated membrane association" @default.
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- W2088303722 doi "https://doi.org/10.1016/j.plantsci.2011.05.016" @default.
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