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- W2088312129 abstract "A simple quantitative micro method is described for the determination of alkaline phosphatase activity on whole homogenates of intestinal mucosal tissue employing the enzymatic hydrolysis of p-nitrophenyl phosphate. Optimal conditions for assay of human intestinal alkaline phosphatase at 30° were found to be 0.75mol 2-amino-2-methyl-1-propanol buffer, pH 10.0, 5 mmol/l substrate, and 50 μl sample volume. Precision of the method was 18.0±0.9 U/ml (mean± standard deviation) on 10 within-day replicates of calf intestinal alkaline phosphatase, electrophoretically purified. Using duodenal biopsy tissue, alkaline phosphatase activity was decreased significantly (p < .001) in a group of 8 children with celiac disease when compared with a group of 10 normal children." @default.
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- W2088312129 date "1970-03-01" @default.
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- W2088312129 title "A micro-technique for the assay of intestinal alkaline phosphatase. results in normal children and in children with celiac disease" @default.
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- W2088312129 doi "https://doi.org/10.1016/s0009-9120(70)80006-6" @default.
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