Matches in SemOpenAlex for { <https://semopenalex.org/work/W2088348092> ?p ?o ?g. }
Showing items 1 to 86 of
86
with 100 items per page.
- W2088348092 endingPage "274" @default.
- W2088348092 startingPage "269" @default.
- W2088348092 abstract "We have developed a quantitative reverse transcription polymerase chain reaction (RT-PCR) method, using both internal and external standardisation, to quantitate fg amounts of pre-pro-thyrotropin releasing hormone (pre-pro-TRH) mRNA in the paraventricular nucleus (PVN) of individual laboratory rats. A constant amount of internal standard is coamplified with both the cDNA of each unknown specimen and a dilution series of an identical RT-PCR generated external standard, allowing quantitation of the samples by interpolation from the external standard curve. Pre-pro-TRH mRNA levels in the PVN were reduced by thyroxine (T4) treatment to 48% of those in control animals, and were increased by thyroidectomy to 155% of the control levels. By combining external and internal standardisation, our method allows multiple samples and treatment groups to be assayed concurrently, thereby eliminating inter-assay variability, whilst retaining the advantages of internal standardisation. It will facilitate further studies of the control of TRH gene expression in pathophysiological conditions." @default.
- W2088348092 created "2016-06-24" @default.
- W2088348092 creator A5076155060 @default.
- W2088348092 creator A5086677527 @default.
- W2088348092 date "1996-10-01" @default.
- W2088348092 modified "2023-09-23" @default.
- W2088348092 title "Quantification of rat pre-pro-thyrotropin releasing hormone (TRH) mRNA by reverse transcription-polymerase chain reaction using external and internal standardisation" @default.
- W2088348092 cites W1967980966 @default.
- W2088348092 cites W1972753801 @default.
- W2088348092 cites W1979796052 @default.
- W2088348092 cites W1983927889 @default.
- W2088348092 cites W2001530093 @default.
- W2088348092 cites W2021953544 @default.
- W2088348092 cites W2039412078 @default.
- W2088348092 cites W2050444807 @default.
- W2088348092 cites W2068262839 @default.
- W2088348092 cites W2085205986 @default.
- W2088348092 cites W2137371141 @default.
- W2088348092 cites W4249176446 @default.
- W2088348092 cites W4294216491 @default.
- W2088348092 doi "https://doi.org/10.1016/0165-0270(96)00090-8" @default.
- W2088348092 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8912200" @default.
- W2088348092 hasPublicationYear "1996" @default.
- W2088348092 type Work @default.
- W2088348092 sameAs 2088348092 @default.
- W2088348092 citedByCount "5" @default.
- W2088348092 countsByYear W20883480922016 @default.
- W2088348092 crossrefType "journal-article" @default.
- W2088348092 hasAuthorship W2088348092A5076155060 @default.
- W2088348092 hasAuthorship W2088348092A5086677527 @default.
- W2088348092 hasConcept C104317684 @default.
- W2088348092 hasConcept C105580179 @default.
- W2088348092 hasConcept C126322002 @default.
- W2088348092 hasConcept C134018914 @default.
- W2088348092 hasConcept C150194340 @default.
- W2088348092 hasConcept C156719811 @default.
- W2088348092 hasConcept C185592680 @default.
- W2088348092 hasConcept C187882448 @default.
- W2088348092 hasConcept C2776216936 @default.
- W2088348092 hasConcept C4692481 @default.
- W2088348092 hasConcept C48023723 @default.
- W2088348092 hasConcept C49105822 @default.
- W2088348092 hasConcept C55493867 @default.
- W2088348092 hasConcept C70784841 @default.
- W2088348092 hasConcept C71315377 @default.
- W2088348092 hasConcept C71924100 @default.
- W2088348092 hasConcept C86803240 @default.
- W2088348092 hasConceptScore W2088348092C104317684 @default.
- W2088348092 hasConceptScore W2088348092C105580179 @default.
- W2088348092 hasConceptScore W2088348092C126322002 @default.
- W2088348092 hasConceptScore W2088348092C134018914 @default.
- W2088348092 hasConceptScore W2088348092C150194340 @default.
- W2088348092 hasConceptScore W2088348092C156719811 @default.
- W2088348092 hasConceptScore W2088348092C185592680 @default.
- W2088348092 hasConceptScore W2088348092C187882448 @default.
- W2088348092 hasConceptScore W2088348092C2776216936 @default.
- W2088348092 hasConceptScore W2088348092C4692481 @default.
- W2088348092 hasConceptScore W2088348092C48023723 @default.
- W2088348092 hasConceptScore W2088348092C49105822 @default.
- W2088348092 hasConceptScore W2088348092C55493867 @default.
- W2088348092 hasConceptScore W2088348092C70784841 @default.
- W2088348092 hasConceptScore W2088348092C71315377 @default.
- W2088348092 hasConceptScore W2088348092C71924100 @default.
- W2088348092 hasConceptScore W2088348092C86803240 @default.
- W2088348092 hasIssue "2" @default.
- W2088348092 hasLocation W20883480921 @default.
- W2088348092 hasLocation W20883480922 @default.
- W2088348092 hasOpenAccess W2088348092 @default.
- W2088348092 hasPrimaryLocation W20883480921 @default.
- W2088348092 hasRelatedWork W2004554921 @default.
- W2088348092 hasRelatedWork W2084054901 @default.
- W2088348092 hasRelatedWork W2145879504 @default.
- W2088348092 hasRelatedWork W2294220327 @default.
- W2088348092 hasRelatedWork W2365524156 @default.
- W2088348092 hasRelatedWork W2370055501 @default.
- W2088348092 hasRelatedWork W2380641360 @default.
- W2088348092 hasRelatedWork W2380799844 @default.
- W2088348092 hasRelatedWork W2393872449 @default.
- W2088348092 hasRelatedWork W2908437812 @default.
- W2088348092 hasVolume "68" @default.
- W2088348092 isParatext "false" @default.
- W2088348092 isRetracted "false" @default.
- W2088348092 magId "2088348092" @default.
- W2088348092 workType "article" @default.