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- W2088349679 abstract "A simple protein determination method is described which gives a linear response from 0.05 to 3–4 mg/ml (or more) of protein and is suitable for dilute (<0.05 mg/ml) protein solutions if concentrated on paper by repeated spotting. Five-microliter solutions of a standard protein and the unknown are spotted on Whatman No. 1 chromatography paper and stained with 0.1% Coomassie brilliant blue R-250. After destaining with water and drying, the stained spots are cut with a cork borer. The dye-protein complex is eluted with 1% SDS, and the absorbance of dye-protein complex is measured at 600 nm. This assay is specific for proteins and virtually free from interference by common laboratory reagents and other nonproteinaceous substances. The color of dye-protein complex is stable for months. There is no specific period of time required for the assay. It can be stopped at a given step, then completed at a more convenient time." @default.
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- W2088349679 date "1978-08-01" @default.
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- W2088349679 title "A simple method for quantitative, semiquantitative, and qualitative assay of protein" @default.
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- W2088349679 doi "https://doi.org/10.1016/0003-2697(78)90749-2" @default.
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