Matches in SemOpenAlex for { <https://semopenalex.org/work/W2088351348> ?p ?o ?g. }
Showing items 1 to 81 of
81
with 100 items per page.
- W2088351348 endingPage "85" @default.
- W2088351348 startingPage "79" @default.
- W2088351348 abstract "The contribution of xanthine oxidoreductase (XDH + XO) to the extracellular release of hydrogen peroxide (H2O2) and intracellular H2O2 concentration in cultured bovine aortic endothelial cells (BAEC) was determined. Intracellular H2O2 concentration was measured by the aminotriazole-mediated inactivation of catalase, while extracellular H2O2 release was measured by the horseradish peroxidase-mediated oxidation of p-hydroxyphenyl acetic acid to a fluorescent dimer. Supplementation of reaction systems with xanthine did not increase H2O2 production by cells. Inhibition of XO activity with allopurinol did not decrease either intracellular concentrations or the extracellular release of H2O2. Similarly, inactivation of XO by culture of cells with tungsten did not have any effect on intracellular levels of H2O2, while it increased extracellular release of H2O2 by 86 and 103% from cells cultured in Medium 199 (M199) and Dulbecco′s modified Eagle′s medium (DMEM), respectively. Cells cultured in DMEM had an average of 8 times greater XDH + XO specific activity, compared to M199 cultured cells, and had a threefold greater rate of release of H2O2 than M199-grown cells. However, DMEM-cultured cells did not have a greater rate of myxothiazole-resistant respiration, suggesting that this increase in H2O2 release comes from sources other than XO. These results show that cellular XO does not contribute significantly to basal H2O2 production in bovine endothelial cells. Analysis of XDH + XO activity of endothelial cells derived from vessels of various species showed a relatively low specific activity of this potential oxidant source in human-derived cells compared with cells cultured from other species such as rodents." @default.
- W2088351348 created "2016-06-24" @default.
- W2088351348 creator A5002611700 @default.
- W2088351348 creator A5013958345 @default.
- W2088351348 creator A5035157985 @default.
- W2088351348 creator A5037451557 @default.
- W2088351348 creator A5038774901 @default.
- W2088351348 creator A5053164982 @default.
- W2088351348 date "1994-05-01" @default.
- W2088351348 modified "2023-09-27" @default.
- W2088351348 title "Endogenous Xanthine Oxidase Does Not Significantly Contribute to Vascular Endothelial Production of Reactive Oxygen Species" @default.
- W2088351348 doi "https://doi.org/10.1006/abbi.1994.1211" @default.
- W2088351348 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8185323" @default.
- W2088351348 hasPublicationYear "1994" @default.
- W2088351348 type Work @default.
- W2088351348 sameAs 2088351348 @default.
- W2088351348 citedByCount "33" @default.
- W2088351348 countsByYear W20883513482012 @default.
- W2088351348 countsByYear W20883513482013 @default.
- W2088351348 countsByYear W20883513482014 @default.
- W2088351348 crossrefType "journal-article" @default.
- W2088351348 hasAuthorship W2088351348A5002611700 @default.
- W2088351348 hasAuthorship W2088351348A5013958345 @default.
- W2088351348 hasAuthorship W2088351348A5035157985 @default.
- W2088351348 hasAuthorship W2088351348A5037451557 @default.
- W2088351348 hasAuthorship W2088351348A5038774901 @default.
- W2088351348 hasAuthorship W2088351348A5053164982 @default.
- W2088351348 hasConcept C123012128 @default.
- W2088351348 hasConcept C153911025 @default.
- W2088351348 hasConcept C181199279 @default.
- W2088351348 hasConcept C185592680 @default.
- W2088351348 hasConcept C202751555 @default.
- W2088351348 hasConcept C2778102346 @default.
- W2088351348 hasConcept C2778979269 @default.
- W2088351348 hasConcept C2779470414 @default.
- W2088351348 hasConcept C28406088 @default.
- W2088351348 hasConcept C48349386 @default.
- W2088351348 hasConcept C533411734 @default.
- W2088351348 hasConcept C54355233 @default.
- W2088351348 hasConcept C55493867 @default.
- W2088351348 hasConcept C79879829 @default.
- W2088351348 hasConcept C81885089 @default.
- W2088351348 hasConcept C86803240 @default.
- W2088351348 hasConceptScore W2088351348C123012128 @default.
- W2088351348 hasConceptScore W2088351348C153911025 @default.
- W2088351348 hasConceptScore W2088351348C181199279 @default.
- W2088351348 hasConceptScore W2088351348C185592680 @default.
- W2088351348 hasConceptScore W2088351348C202751555 @default.
- W2088351348 hasConceptScore W2088351348C2778102346 @default.
- W2088351348 hasConceptScore W2088351348C2778979269 @default.
- W2088351348 hasConceptScore W2088351348C2779470414 @default.
- W2088351348 hasConceptScore W2088351348C28406088 @default.
- W2088351348 hasConceptScore W2088351348C48349386 @default.
- W2088351348 hasConceptScore W2088351348C533411734 @default.
- W2088351348 hasConceptScore W2088351348C54355233 @default.
- W2088351348 hasConceptScore W2088351348C55493867 @default.
- W2088351348 hasConceptScore W2088351348C79879829 @default.
- W2088351348 hasConceptScore W2088351348C81885089 @default.
- W2088351348 hasConceptScore W2088351348C86803240 @default.
- W2088351348 hasIssue "1" @default.
- W2088351348 hasLocation W20883513481 @default.
- W2088351348 hasLocation W20883513482 @default.
- W2088351348 hasOpenAccess W2088351348 @default.
- W2088351348 hasPrimaryLocation W20883513481 @default.
- W2088351348 hasRelatedWork W1914788654 @default.
- W2088351348 hasRelatedWork W1985452104 @default.
- W2088351348 hasRelatedWork W1998417021 @default.
- W2088351348 hasRelatedWork W2038275609 @default.
- W2088351348 hasRelatedWork W2055850521 @default.
- W2088351348 hasRelatedWork W2080568301 @default.
- W2088351348 hasRelatedWork W2361812330 @default.
- W2088351348 hasRelatedWork W2374558055 @default.
- W2088351348 hasRelatedWork W2463125990 @default.
- W2088351348 hasRelatedWork W3214962737 @default.
- W2088351348 hasVolume "311" @default.
- W2088351348 isParatext "false" @default.
- W2088351348 isRetracted "false" @default.
- W2088351348 magId "2088351348" @default.
- W2088351348 workType "article" @default.