FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2088360693> ?p ?o ?g. }

• W2088360693 endingPage "139" @default.
• W2088360693 startingPage "131" @default.
• W2088360693 abstract "We have shown that a conjugate of wheat germ agglutinin (WGA), with horseradish peroxidase (HRP), is more sensitive than native HRP as a probe of neuroanatomic connections involving the retrograde transport of the lectin. It has also been shown in our laboratory that WGA-HRP remains at the site of injection twice as long as HRP. The purpose of the present morphometric study was to investigate the basis for the higher sensitivity of WGA-HRP over HRP as a retrogradely transported tracer molecule. To do this, we modified the experiment of Heuser and Reese which utilized the tracing of HRP in the frog neuromuscular junction (Heuser, J.E. and Reese, T.S., J. Cell Biol., 57 (1973) 315–344). Instead of using HRP alone, we examined, in double labeling experiments, fluid and adsorptive endocytosis with free HRP and WGA coupled to ferritin (WGA-ferritin) respectively. Immediately after nerve stimulation, both markers are taken up simultaneously into cisternae, and in tubular structures strikingly similar to the described compartment of uncoupling of receptor from ligand (CURL). Frequently, cisternae were connected with putative CURL. This early double labeling of cisternae and putative CURL was followed by the appearance of synaptic vesicles labeled with WGA-ferritin only (72–79%), HRP only (6–11%), and both labels (13–16%). In contrast to the labeling pattern of synaptic vesicles, the majority of cisternae and putative CURL had both labels throughout the duration of the experiments (77–80%). The results of this study indicate that most of WGA-ferritin and HRP are co-localized in cisternae and putative CURL, compartments involved in endocytosis and surface receptor recycling. Therefore, the superior sensitivity of WGA over HRP as a marker of retrograde transport cannot be attributed to a differential distribution of these two tracer molecules within the intracellular compartment involved in the endocytosis of ligands. These results are consistent with the hypothesis that in the nerve ending, as in the cell body, there is a compartment involved in the recycling of surface receptors. Such receptors may include molecules with terminal sialic acid and N-acetylglucosamine to which WGA binds. This compartment may be involved in numerous cycles of endocytosis of WGA bound to the surface of the nerve ending until the surface bound and extracellular pool of the lectin is exhausted. It remains to be proven whether in the frog presynaptic terminal the compartment identified in this study as ‘putative CURL’ is indeed involved in the recycling of cell surface receptors." @default.
• W2088360693 created "2016-06-24" @default.
• W2088360693 creator A5021802586 @default.
• W2088360693 creator A5025285910 @default.
• W2088360693 creator A5025521495 @default.
• W2088360693 date "1989-08-01" @default.
• W2088360693 modified "2023-09-24" @default.
• W2088360693 title "A hypothesis for the superior sensitivity of wheat germ agglutinin as a neuroanatomical probe" @default.
• W2088360693 cites W1965623651 @default.
• W2088360693 cites W1982668160 @default.
• W2088360693 cites W1985545197 @default.
• W2088360693 cites W1988602065 @default.
• W2088360693 cites W1992337930 @default.
• W2088360693 cites W2012846132 @default.
• W2088360693 cites W2033958192 @default.
• W2088360693 cites W2036983417 @default.
• W2088360693 cites W2044385880 @default.
• W2088360693 cites W2054148926 @default.
• W2088360693 cites W2068672470 @default.
• W2088360693 cites W2079628485 @default.
• W2088360693 cites W2080726191 @default.
• W2088360693 cites W2090498350 @default.
• W2088360693 cites W2099826607 @default.
• W2088360693 cites W2117312662 @default.
• W2088360693 cites W2122148108 @default.
• W2088360693 cites W2149938812 @default.
• W2088360693 cites W2151556889 @default.
• W2088360693 cites W2172095199 @default.
• W2088360693 cites W2399392655 @default.
• W2088360693 doi "https://doi.org/10.1016/0006-8993(89)91226-2" @default.
• W2088360693 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/2789087" @default.
• W2088360693 hasPublicationYear "1989" @default.
• W2088360693 type Work @default.
• W2088360693 sameAs 2088360693 @default.
• W2088360693 citedByCount "9" @default.
• W2088360693 countsByYear W20883606932018 @default.
• W2088360693 crossrefType "journal-article" @default.
• W2088360693 hasAuthorship W2088360693A5021802586 @default.
• W2088360693 hasAuthorship W2088360693A5025285910 @default.
• W2088360693 hasAuthorship W2088360693A5025521495 @default.
• W2088360693 hasConcept C105702510 @default.
• W2088360693 hasConcept C12554922 @default.
• W2088360693 hasConcept C130316041 @default.
• W2088360693 hasConcept C181199279 @default.
• W2088360693 hasConcept C185592680 @default.
• W2088360693 hasConcept C2777676563 @default.
• W2088360693 hasConcept C2778319317 @default.
• W2088360693 hasConcept C2780175098 @default.
• W2088360693 hasConcept C2780758401 @default.
• W2088360693 hasConcept C41625074 @default.
• W2088360693 hasConcept C55493867 @default.
• W2088360693 hasConcept C70192387 @default.
• W2088360693 hasConcept C86803240 @default.
• W2088360693 hasConcept C95444343 @default.
• W2088360693 hasConceptScore W2088360693C105702510 @default.
• W2088360693 hasConceptScore W2088360693C12554922 @default.
• W2088360693 hasConceptScore W2088360693C130316041 @default.
• W2088360693 hasConceptScore W2088360693C181199279 @default.
• W2088360693 hasConceptScore W2088360693C185592680 @default.
• W2088360693 hasConceptScore W2088360693C2777676563 @default.
• W2088360693 hasConceptScore W2088360693C2778319317 @default.
• W2088360693 hasConceptScore W2088360693C2780175098 @default.
• W2088360693 hasConceptScore W2088360693C2780758401 @default.
• W2088360693 hasConceptScore W2088360693C41625074 @default.
• W2088360693 hasConceptScore W2088360693C55493867 @default.
• W2088360693 hasConceptScore W2088360693C70192387 @default.
• W2088360693 hasConceptScore W2088360693C86803240 @default.
• W2088360693 hasConceptScore W2088360693C95444343 @default.
• W2088360693 hasIssue "1" @default.
• W2088360693 hasLocation W20883606931 @default.
• W2088360693 hasLocation W20883606932 @default.
• W2088360693 hasOpenAccess W2088360693 @default.
• W2088360693 hasPrimaryLocation W20883606931 @default.
• W2088360693 hasRelatedWork W1974996653 @default.
• W2088360693 hasRelatedWork W1995141793 @default.
• W2088360693 hasRelatedWork W1996231004 @default.
• W2088360693 hasRelatedWork W2003898664 @default.
• W2088360693 hasRelatedWork W2035663687 @default.
• W2088360693 hasRelatedWork W2036983417 @default.
• W2088360693 hasRelatedWork W2043003171 @default.
• W2088360693 hasRelatedWork W2055237298 @default.
• W2088360693 hasRelatedWork W2087340288 @default.
• W2088360693 hasRelatedWork W2133569944 @default.
• W2088360693 hasVolume "495" @default.
• W2088360693 isParatext "false" @default.
• W2088360693 isRetracted "false" @default.
• W2088360693 magId "2088360693" @default.
• W2088360693 workType "article" @default.