Matches in SemOpenAlex for { <https://semopenalex.org/work/W2088404799> ?p ?o ?g. }
Showing items 1 to 96 of
96
with 100 items per page.
- W2088404799 endingPage "155" @default.
- W2088404799 startingPage "145" @default.
- W2088404799 abstract "The arginine deiminase (AD) gene was cloned from Mycoplasma arginini and expressed in the cytosol of Escherichia coli as inclusion bodies with an expression level of at least 20% of the total bacterial proteins. The inclusion bodies were solubilized with 6 M guanidine hydrochloride (Gdn-HCl) under reducing conditions, in order to avoid incorrect disulfide-bond formation of the recombinant (r-) AD molecules, and renaturation was performed under various refolding conditions. The optimum renaturation conditions were found to be incubation for 90 h at pH 7.5 and 15°C. The resulting completely refolded r-AD was purified to homogeneity by anion-exchange and arginine-affinity chromatography and its activity yield was 72.5%. The specific activity of the purified r-AD was comparable to and its amino acid composition was identical to those of Mycoplasma AD, and NH2-terminal sequence analysis revealed that its methionine residue corresponding to the translation initiation codon had been removed completely. Anti-tumor activity analyses showed that r-AD inhibited the growth of two mouse cell lines, hepatoma MH134 and fibrosarcoma Meth A, strongly in vitro at concentrations in excess of 10 ng ml−1. Moreover, when MH134-implanted mice were given single intravenous injections of r-AD at doses of 50 mg kg−1 and higher, their survival times were prolonged significantly. These results, taken together, indicate that the enzymatic properties and biological actions of r-AD were highly consistent with those of Mycoplasma AD." @default.
- W2088404799 created "2016-06-24" @default.
- W2088404799 creator A5005778977 @default.
- W2088404799 creator A5007771940 @default.
- W2088404799 creator A5026003800 @default.
- W2088404799 creator A5054161366 @default.
- W2088404799 creator A5082867684 @default.
- W2088404799 date "1994-08-01" @default.
- W2088404799 modified "2023-09-28" @default.
- W2088404799 title "High-level expression of Mycoplasma arginine deiminase in Escherichia coli and its efficient renaturation as an anti-tumor enzyme" @default.
- W2088404799 cites W1011002578 @default.
- W2088404799 cites W1011692429 @default.
- W2088404799 cites W1528400386 @default.
- W2088404799 cites W1553888996 @default.
- W2088404799 cites W1966596437 @default.
- W2088404799 cites W1969211062 @default.
- W2088404799 cites W1973241521 @default.
- W2088404799 cites W1990818641 @default.
- W2088404799 cites W1992033102 @default.
- W2088404799 cites W1997516365 @default.
- W2088404799 cites W2009413592 @default.
- W2088404799 cites W2019470388 @default.
- W2088404799 cites W2027603263 @default.
- W2088404799 cites W2028622989 @default.
- W2088404799 cites W2044062335 @default.
- W2088404799 cites W2064691821 @default.
- W2088404799 cites W2107473126 @default.
- W2088404799 cites W2110655401 @default.
- W2088404799 doi "https://doi.org/10.1016/0168-1656(94)90050-7" @default.
- W2088404799 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/7765234" @default.
- W2088404799 hasPublicationYear "1994" @default.
- W2088404799 type Work @default.
- W2088404799 sameAs 2088404799 @default.
- W2088404799 citedByCount "56" @default.
- W2088404799 countsByYear W20884047992014 @default.
- W2088404799 countsByYear W20884047992015 @default.
- W2088404799 countsByYear W20884047992017 @default.
- W2088404799 countsByYear W20884047992019 @default.
- W2088404799 countsByYear W20884047992020 @default.
- W2088404799 countsByYear W20884047992021 @default.
- W2088404799 countsByYear W20884047992022 @default.
- W2088404799 countsByYear W20884047992023 @default.
- W2088404799 crossrefType "journal-article" @default.
- W2088404799 hasAuthorship W2088404799A5005778977 @default.
- W2088404799 hasAuthorship W2088404799A5007771940 @default.
- W2088404799 hasAuthorship W2088404799A5026003800 @default.
- W2088404799 hasAuthorship W2088404799A5054161366 @default.
- W2088404799 hasAuthorship W2088404799A5082867684 @default.
- W2088404799 hasConcept C104317684 @default.
- W2088404799 hasConcept C153911025 @default.
- W2088404799 hasConcept C181199279 @default.
- W2088404799 hasConcept C185592680 @default.
- W2088404799 hasConcept C203750385 @default.
- W2088404799 hasConcept C2777186326 @default.
- W2088404799 hasConcept C2777468819 @default.
- W2088404799 hasConcept C2780912031 @default.
- W2088404799 hasConcept C40767141 @default.
- W2088404799 hasConcept C515207424 @default.
- W2088404799 hasConcept C547475151 @default.
- W2088404799 hasConcept C55493867 @default.
- W2088404799 hasConcept C86803240 @default.
- W2088404799 hasConceptScore W2088404799C104317684 @default.
- W2088404799 hasConceptScore W2088404799C153911025 @default.
- W2088404799 hasConceptScore W2088404799C181199279 @default.
- W2088404799 hasConceptScore W2088404799C185592680 @default.
- W2088404799 hasConceptScore W2088404799C203750385 @default.
- W2088404799 hasConceptScore W2088404799C2777186326 @default.
- W2088404799 hasConceptScore W2088404799C2777468819 @default.
- W2088404799 hasConceptScore W2088404799C2780912031 @default.
- W2088404799 hasConceptScore W2088404799C40767141 @default.
- W2088404799 hasConceptScore W2088404799C515207424 @default.
- W2088404799 hasConceptScore W2088404799C547475151 @default.
- W2088404799 hasConceptScore W2088404799C55493867 @default.
- W2088404799 hasConceptScore W2088404799C86803240 @default.
- W2088404799 hasIssue "2" @default.
- W2088404799 hasLocation W20884047991 @default.
- W2088404799 hasLocation W20884047992 @default.
- W2088404799 hasOpenAccess W2088404799 @default.
- W2088404799 hasPrimaryLocation W20884047991 @default.
- W2088404799 hasRelatedWork W1964117139 @default.
- W2088404799 hasRelatedWork W1967665958 @default.
- W2088404799 hasRelatedWork W1986909607 @default.
- W2088404799 hasRelatedWork W2007930265 @default.
- W2088404799 hasRelatedWork W2019922031 @default.
- W2088404799 hasRelatedWork W2025468017 @default.
- W2088404799 hasRelatedWork W2092993483 @default.
- W2088404799 hasRelatedWork W2121551754 @default.
- W2088404799 hasRelatedWork W2238024296 @default.
- W2088404799 hasRelatedWork W3144801213 @default.
- W2088404799 hasVolume "36" @default.
- W2088404799 isParatext "false" @default.
- W2088404799 isRetracted "false" @default.
- W2088404799 magId "2088404799" @default.
- W2088404799 workType "article" @default.