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- W2088406735 abstract "A variety of chromatographic techniques, for example, open-column chromatography, thin-layer chromatography (TLC), and gas chromatography (GC), have been developed for the isolation and identification of prostaglandins (PGs), (thromboxanes) TXs, hydroxy fatty acids, and leukotrienes (LTs) (these are generically known as prostanoids). The open-column chromatographic technique is usually used for the crude extraction of prostanoids from biological samples. Although, TLC is the most commonly and readily used method for the pretreatment and identification of prostanoids, the resolution and recoveries are poor. GC is also frequently utilized in the separation of many prostanoids. Although, this technique requires derivatization in order to obtain thermal stability and volatility, some methoxime derivatives, for example, PGA, PGD and PGE, lead to double peaks due to syn and anti isomers. High-performance liquid chromatography (HPLC) has been used, increasingly in the recent years for the isolation and purification of prostanoids from biological samples in place of TLC and GC. This method is very useful for the simultaneous separation of the metabolic products of both the cyclooxygenase and lipoxygenase pathways. High-efficiency columns have made possible many separations of prostanoids, and good recoveries and reproducibility are easily obtained." @default.
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- W2088406735 date "1975-11-01" @default.
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- W2088406735 title "Separationof methyl esters of polyunsaturated fatty acids by argentation thin-layer chromatography" @default.
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- W2088406735 doi "https://doi.org/10.1016/s0021-9673(00)85279-6" @default.
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