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- W2088406776 abstract "Abstract A convenient, quantitative assay method of branching enzyme (BE) was devised with reduced amylose as the substrate. Using this assay, the properties of the purified branching isoenzymes from maize, BE I, IIa, and IIb, were studied. The method is based on determination of reducing power, by the modified Park-Johnson method, of the chains transferred by BE after they are released from the branched products with isoamylase. The optimum pH of the three enzymes is 7.5, and the optimum temperatures of BE I, IIa, and IIb are 33, 25, and 15–20°C, respectively. The specific activities are found to be the highest for BE I and the lowest for BE IIb, whereas in the conventional assay based on stimulation of unprimed phosphorylase activity, the specific activities are BE IIb > 11a > I. BE I has a lower Km (2.0 μM of the nonreducing terminal) for the reduced amylose of average chain-length ( cl ) 405 than BE IIa (10 μM) and IIb (11μM), and the enzyme shows a higher Km for reduced amyloses of smaller cl . Gel-permeation chromatograms on Sephadex G-75SF of the chains transferred from the reduced amylose indicate that initially the three isoenzymes produce chains of various sizes (dp ∼ 8 to > 200), and BE I preferentially transfers longer chains than BE IIa and IIb." @default.
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- W2088406776 date "1993-02-01" @default.
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- W2088406776 title "Branching of amylose by the branching isoenzymes of maize endosperm" @default.
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- W2088406776 doi "https://doi.org/10.1016/0008-6215(93)84188-c" @default.
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