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- W2088440189 abstract "The antigen specific stimulation of DNA synthesis in spleen cell suspensions from immunized rabbits was used as a measure of antigen activity. The effect of modification of antigen on antigenic activity was studied. Bovine serum albumin was tested following dinitrophenylation, disruption of disulphide bonds and acylation with a variety of reagents. In general, most chemical procedures apparently did not abolish immunogenicity. Results with dinitrophenylated derivatives, however, demonstrated that the immunogenic activity of a chemically modified protein is determined by both its physical and chemical parameters. The importance of the physical nature of the molecule was also demonstrated in experiments where grossly denatured preparations were tested in the in vitro system; highly aggregated preparations were the most active." @default.
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- W2088440189 date "1967-11-01" @default.
- W2088440189 modified "2023-09-24" @default.
- W2088440189 title "The effect of physical and chemical modifications on antigen in the secondary response in vitro" @default.
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- W2088440189 doi "https://doi.org/10.1016/0019-2791(67)90101-2" @default.
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