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- W2088444966 abstract "A high-performance liquid chromatographic method for the determination of trimethoprim metabolites in pig urine was developed. The metabolites — glucuronic acid and sulphuric acid conjugates of phenolic metabolites formed by demethylation of trimethoprim — were quantitated after treatment of urine with β-glucuronidase (Escherichia coli). The sulphuric acid conjugate was not susceptible to enzymatic hydrolysis and was therefore assayed as the conjugate by use of ion-pair chromatography on the reversed-phase column. In order to find suitable conditions for enzymatic hydrolysis of the glucoronides, the conjugates were obtained by gel chromatography of urine from a [14C]trimethoprim-treated pig." @default.
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- W2088444966 date "1984-01-01" @default.
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- W2088444966 title "Determination of trimethoprim metabolites including conjugates in urine using high-performance liquid chromatography with combined ultraviolet and electrochemical detection" @default.
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- W2088444966 doi "https://doi.org/10.1016/s0378-4347(00)83353-8" @default.
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