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- W2088462598 abstract "This study presents the effect of pore regularity on the preservation and differentiation of bone marrow stromal cells (BMSCs). Scaffolds with interconnected pores of inverted colloidal crystal (ICC) geometry were prepared by infiltrating chitosan–gelatin gels into the interstices of self-assembled microspheres, which were later dissolved with a solvent. In addition, the pore surfaces were grafted with two laminin-derived peptides (LDP). The experimental results revealed that the number of BMSCs in ICC scaffolds could increase 2.7-fold after cultivation over 7 days. Moreover, the distribution of cultured BMSCs in ICC scaffolds was quite uniform as compared with freeform scaffolds. ICC scaffolds could preserve 63% phenotypic BMSCs in average and freeform scaffolds 56%. The grafted LDP enhanced the adhesion efficiency of BMSCs in ICC scaffolds (about 70–75%) and produced NeuN-positive cells. A further induction with neuron growth factor could guide the differentiation of BMSCs toward mature neurons in LDP-grafted ICC scaffolds. The controlled topography of ICC structure and surface LDP can be promising in the cultivation of BMSCs and neural regeneration." @default.
- W2088462598 created "2016-06-24" @default.
- W2088462598 creator A5000670353 @default.
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- W2088462598 date "2011-01-01" @default.
- W2088462598 modified "2023-09-27" @default.
- W2088462598 title "Inverted colloidal crystal scaffolds with laminin-derived peptides for neuronal differentiation of bone marrow stromal cells" @default.
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- W2088462598 doi "https://doi.org/10.1016/j.biomaterials.2010.09.057" @default.
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