FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2088475484> ?p ?o ?g. }

• W2088475484 endingPage "77" @default.
• W2088475484 startingPage "64" @default.
• W2088475484 abstract "A series of 5′ deletion test plasmids barboring promoter sequences of the HLA-DQβ gene fused to the bacterial chloramphenicol acetyl transferase (CAT) gene were constructed. Transient CAT expression from these constructs in several types of cells was employed to examine the role of the promoter sequence in the regulation of DQß gene expression. The DQß constructs drove CAT expression in Raji cells (human Burkitt lymphoma cells) to at least 25-fold or 50-fold higher levels than in Hela cells (human cervical carcinoma cells) or Jurkat cells (human T-leukemia cells), respectively. A short promoter sequence of −160 bp containing the conserved × and Y sequences was sufficient for expression in Raji cells, and deletion to −106 bp which interrupted the × sequence abolished the expression. Sequences further upstream to −160 bp as far as −2500 bp which included an Ig-like octamer appeared to have no effect on expression in Raji cells. Thus, the promoter up to −160 bp has all of the sequences required for B cell specific expression of CAT in this assay. CAT expression from the 5′ deletion constructs introduced into RJ2.2.5 and 6.1.6 cells (class II-negative mutant B cells Lines) was also examined. None of the 5′ deletion constructs, including that with −160 bp of promoter, showed CAT expression in these cells, suggesting that a transcriptional factor(s) required for the activity of the DQß promoter was missing m these cells. Moreover, the −160 to −66 bp sequence (including the × and Y elements), which functions as a B cell specific enhancer, was inactive in the mutant cells. Thus, the missing factor(s) are required for the enhancer function of this fragment." @default.
• W2088475484 created "2016-06-24" @default.
• W2088475484 creator A5040129865 @default.
• W2088475484 creator A5069704242 @default.
• W2088475484 date "1989-07-01" @default.
• W2088475484 modified "2023-09-24" @default.
• W2088475484 title "Studies of expression of the DQß promoter and its 5′ deletion derivatives in normal and mutant human B cell lines" @default.
• W2088475484 cites W1474595909 @default.
• W2088475484 cites W1515272472 @default.
• W2088475484 cites W1566285692 @default.
• W2088475484 cites W1591208477 @default.
• W2088475484 cites W1830661192 @default.
• W2088475484 cites W18949849 @default.
• W2088475484 cites W1954929088 @default.
• W2088475484 cites W1969178843 @default.
• W2088475484 cites W1973204882 @default.
• W2088475484 cites W1978439483 @default.
• W2088475484 cites W1978881490 @default.
• W2088475484 cites W1984150554 @default.
• W2088475484 cites W1993280477 @default.
• W2088475484 cites W1996527034 @default.
• W2088475484 cites W1998844870 @default.
• W2088475484 cites W2001187632 @default.
• W2088475484 cites W2003008067 @default.
• W2088475484 cites W2004256051 @default.
• W2088475484 cites W2010356985 @default.
• W2088475484 cites W2018435845 @default.
• W2088475484 cites W2023815335 @default.
• W2088475484 cites W2031300940 @default.
• W2088475484 cites W2038563280 @default.
• W2088475484 cites W2038715190 @default.
• W2088475484 cites W2045917505 @default.
• W2088475484 cites W2051470342 @default.
• W2088475484 cites W2056526609 @default.
• W2088475484 cites W2065564934 @default.
• W2088475484 cites W2067956561 @default.
• W2088475484 cites W2074198853 @default.
• W2088475484 cites W2077708499 @default.
• W2088475484 cites W2079712794 @default.
• W2088475484 cites W2081135713 @default.
• W2088475484 cites W2089593395 @default.
• W2088475484 cites W2092521827 @default.
• W2088475484 cites W2094438122 @default.
• W2088475484 cites W2094571268 @default.
• W2088475484 cites W2105147698 @default.
• W2088475484 cites W2125228907 @default.
• W2088475484 cites W2135277567 @default.
• W2088475484 cites W2138234074 @default.
• W2088475484 cites W2144206181 @default.
• W2088475484 cites W2149563853 @default.
• W2088475484 cites W2151920048 @default.
• W2088475484 cites W330744992 @default.
• W2088475484 cites W4244433501 @default.
• W2088475484 cites W4362215246 @default.
• W2088475484 doi "https://doi.org/10.1111/j.1399-0039.1989.tb01718.x" @default.
• W2088475484 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/2510365" @default.
• W2088475484 hasPublicationYear "1989" @default.
• W2088475484 type Work @default.
• W2088475484 sameAs 2088475484 @default.
• W2088475484 citedByCount "10" @default.
• W2088475484 crossrefType "journal-article" @default.
• W2088475484 hasAuthorship W2088475484A5040129865 @default.
• W2088475484 hasAuthorship W2088475484A5069704242 @default.
• W2088475484 hasConcept C101762097 @default.
• W2088475484 hasConcept C104317684 @default.
• W2088475484 hasConcept C143065580 @default.
• W2088475484 hasConcept C150194340 @default.
• W2088475484 hasConcept C153911025 @default.
• W2088475484 hasConcept C161733203 @default.
• W2088475484 hasConcept C16232356 @default.
• W2088475484 hasConcept C179464577 @default.
• W2088475484 hasConcept C2776090121 @default.
• W2088475484 hasConcept C2777366897 @default.
• W2088475484 hasConcept C2780730277 @default.
• W2088475484 hasConcept C33161422 @default.
• W2088475484 hasConcept C40767141 @default.
• W2088475484 hasConcept C54009773 @default.
• W2088475484 hasConcept C54355233 @default.
• W2088475484 hasConcept C81885089 @default.
• W2088475484 hasConcept C86803240 @default.
• W2088475484 hasConcept C8891405 @default.
• W2088475484 hasConceptScore W2088475484C101762097 @default.
• W2088475484 hasConceptScore W2088475484C104317684 @default.
• W2088475484 hasConceptScore W2088475484C143065580 @default.
• W2088475484 hasConceptScore W2088475484C150194340 @default.
• W2088475484 hasConceptScore W2088475484C153911025 @default.
• W2088475484 hasConceptScore W2088475484C161733203 @default.
• W2088475484 hasConceptScore W2088475484C16232356 @default.
• W2088475484 hasConceptScore W2088475484C179464577 @default.
• W2088475484 hasConceptScore W2088475484C2776090121 @default.
• W2088475484 hasConceptScore W2088475484C2777366897 @default.
• W2088475484 hasConceptScore W2088475484C2780730277 @default.
• W2088475484 hasConceptScore W2088475484C33161422 @default.
• W2088475484 hasConceptScore W2088475484C40767141 @default.
• W2088475484 hasConceptScore W2088475484C54009773 @default.
• W2088475484 hasConceptScore W2088475484C54355233 @default.
• W2088475484 hasConceptScore W2088475484C81885089 @default.
• W2088475484 hasConceptScore W2088475484C86803240 @default.

• next