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- W2088528360 abstract "The filamentous fungus Aspergillusochraceus TS produces an inducible microsomal cytochrome P-450 linked monooxygenase which is capable of hydroxylating benzo(a)pyrene in presence of O2 and NADPH. The addition of Benzo(a)pyrene, 3-Methyl cholanthrene, β-Napthoflavone and other aryl hydrocarbons during the induction period causes drammatic improvement in the kinetics of benzo(a) pyrene hydroxylation as was evidenced by large decrease in Km and increase in Vmax values. On the other hand, treatment with Phenobarbital, Polychlorinated biphenyl and Progesterone has no significant effect on the kinetics of benzo(a)pyrene hydroxylation although a significant induction of NADPH-Cyt C reductase activity was observed in all the three cases. Again, both Phenobarbital and 3-Methyl cholanthrene induced microsomes exhibit the characteristic reduced metyrapone difference spectra. These findings together with the results obtained with flavone on the metabolism of benzo(a) pyrene by various microsomal preparations suggest a parallel induction of multiple forms of cytochrome P-450 as observed in mammalian liver under identical condition." @default.
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- W2088528360 date "1983-09-01" @default.
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- W2088528360 title "Induction of benzo(a)pyrene hydroxylase in aspergillusochraceus TS: Evidences of multiple forms of cytochrome P-450" @default.
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- W2088528360 doi "https://doi.org/10.1016/s0006-291x(83)80200-9" @default.
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