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- W2088551091 abstract "4-Aminophenol was selected as a model hapten for thermal-lens detection in a heterogeneous indirect immunoassay for small organic compounds. The assay is based on the competition of the free hapten and 4-aminophenol labeled with tetramethyl rhodamine isothiocyanate (TRITC) for rabbit anti-4-aminophenol antibodies (4-AP-GA-BSA) immobilized on a transparent poly(ethylene terephthalate) plate. The amount of the colored analyte was directly measured by thermal lensing at the plate surface. The developed method is specific for 4-aminophenol. The limit of detection is 2 × 10 −7 M, which corresponds to the absolute amount of 3 × 10 −11 mol of 4-aminophenol. The sensitivities of the developed thermal-lens and fluorescence-based measurements of the immunoassay under the same conditions are in good agreement with the expected and discussed theoretical sensitivities of photothermal and fluorescence measurements." @default.
- W2088551091 created "2016-06-24" @default.
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- W2088551091 date "2010-08-01" @default.
- W2088551091 modified "2023-10-14" @default.
- W2088551091 title "Heterogeneous Thermal-Lens Immunoassay for Small Organic Compounds: Determination of 4-Aminophenol" @default.
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- W2088551091 doi "https://doi.org/10.1366/000370210792081136" @default.
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